Описание: Part I: Background and Overview
1. Structural Biology of Nonribosomal Peptide Synthetases
Bradley R. Miller and Andrew M. Gulick
2. The Assembly Line Enzymology of Polyketide Biosynthesis
Marisa Till and Paul R. Race
Part II: In Vitro Methods
3. Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay
Benjamin P. Duckworth, Daniel J. Wilson, and Courtney C. Aldrich
4. Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains
Fumihiro Ishikawa and Hideaki Kakeya
5. Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases
Chitose Maruyama, Haruka Niikura, Masahiro Takakuwa, Hajime Katano, and Yoshimitsu Hamano
6. Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis
Clara Brieke, Veronika Kratzig, Madeleine Peschke, and Max J. Cryle
7. Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro
Ralph A. Cacho and Yi Tang
8. The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis
Haoran Zhang, Lei Fang, Marcia S. Osburne, and Blaine A. Pfeifer
9. Screening for Expressed Non
ribosomal Peptide Synthetases and Polyketide Synthases Using LC‒MS/MS-Based Proteomics Yunqiu Chen, Ryan A. McClure, and Neil L. Kelleher
10. Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi
Alexandra A. Soukup, Nancy P. Keller, and Philipp Wiemann
11. In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
Delphine Debois, Marc Ongena, Hйlиne Cawoy, and Edwin De Pauw
12. Secondary Metabolic Pathway-Targeted Metabolomics
Maria I. Vizcaino and Jason M. Crawford
Part III: Bioinformatic Methods
13. Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra
