Автор: Bradley Evans Название: Nonribosomal Peptide and Polyketide Biosynthesis ISBN: 1493980343 ISBN-13(EAN): 9781493980345 Издательство: Springer Рейтинг: Цена: 93160.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Part I: Background and Overview
1. Structural Biology of Nonribosomal Peptide Synthetases
Bradley R. Miller and Andrew M. Gulick
2. The Assembly Line Enzymology of Polyketide Biosynthesis
Marisa Till and Paul R. Race
Part II: In Vitro Methods
3. Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay
Benjamin P. Duckworth, Daniel J. Wilson, and Courtney C. Aldrich
4. Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains
Fumihiro Ishikawa and Hideaki Kakeya
5. Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases
6. Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis
Clara Brieke, Veronika Kratzig, Madeleine Peschke, and Max J. Cryle
7. Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro
Ralph A. Cacho and Yi Tang
8. The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis
Haoran Zhang, Lei Fang, Marcia S. Osburne, and Blaine A. Pfeifer
9. Screening for Expressed Non
ribosomal Peptide Synthetases and Polyketide Synthases Using LC‒MS/MS-Based Proteomics
Yunqiu Chen, Ryan A. McClure, and Neil L. Kelleher
10. Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi
Alexandra A. Soukup, Nancy P. Keller, and Philipp Wiemann
11. In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
Delphine Debois, Marc Ongena, Hйlиne Cawoy, and Edwin De Pauw
13. Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra
14. Bioinformatics Tools for the Discovery of New Nonribosomal Peptides
Valйrie Leclиre, Tilmann Weber, Philippe Jacques, and Maude Pupin
15. The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways
Nicolas Tremblay, Patrick Hill, Kyle R. Conway, and Christopher N. Boddy
16. Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains
Guillermin Agьero-Chapin, Gisselle Pйrez-Machado, Aminael Sбnchez-Rodrнguez, Miguel Machado Santos, and Agostinho Antunes
17. Characterization of Nonribosomal Peptide Synthetases with NRPSsp
C
arlos Prieto
Автор: Bradley Evans Название: Nonribosomal Peptide and Polyketide Biosynthesis ISBN: 1493933736 ISBN-13(EAN): 9781493933730 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function. It then continues with methods for the analysis of these pathways including conventional enzymological assays, contemporary mass spectrometric analysis techniques, specialized molecular biological approaches applicable to NRPSs and PKSs, and small molecule analysis tools tailored to this very special class of natural products, and concludes by examining bioinformatics tools for the analysis of these enzymes, pathways, and molecules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nonribosomal Peptide and Polyketide Biosynthesis: Methods and Protocols serves as a valuable reference for those experienced in studying NRPS and PKS enzymes, pathways, and natural products as well as a gateway for those just entering the field.
Автор: Utpal Chandra De, Priyambada Mishra, Prasenjit Rudra Pal, Ajoy Basak Название: Non-peptide Inhibitors of Proprotein Convertase Subtilisin Kexins (PCSKs): An Overall Review of Existing and New Data ISBN: 1615044744 ISBN-13(EAN): 9781615044740 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 41580.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Provides an overall review of all non-peptide PCSK inhibitors so far reported in the literature along with those identified recently for the first time and not yet published. The potential implications of these molecules as biochemical, therapeutical, or clinical agents is also discussed.
Автор: Abdel-Majid Khatib Название: Regulation of Carcinogenesis, Angiogenesis and Metastasis by the Proprotein Convertases (PC`s) ISBN: 9401780900 ISBN-13(EAN): 9789401780902 Издательство: Springer Рейтинг: Цена: 149060.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Convertases are widely expressed activating enzymes involved in various physiological and pathological processes. The purpose of this book is to provide detailed and updated information on the role of these molecules in cancer. This book is the first to summarize current knowledge about the importance of protein precursors maturation by the convertases in tumor progression, angiogenesis and metastasis. In each chapter, the importance of the convertases in the activation of various cancer-related molecules including growth factors, adhesion molecules and proteases is discussed. Also this book covers the role of some convertases in the clinical setting. The book will be of interest to basic researchers as well as clinicians. It will also interest those working in the cancer research area and the pharmaceutical industry, who are looking for new strategies in cancer therapy. Finally, this book is also a useful reference for researchers and clinicians pursuing research on growth factors, adhesion molecules, proteases and their implications in the processes of tumor progression, angiogenesis and metastasis.
Автор: Mbikay Majambu, Seidah Nabil G. Название: Proprotein Convertases ISBN: 1493961764 ISBN-13(EAN): 9781493961764 Издательство: Springer Рейтинг: Цена: 135090.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Today, activation endoproteolysis of secretory proteins is recognized as a fundamental biological mechanism of spatial and temporal regulation of protein activity as well as of diversification of protein functions. In Proprotein Convertases, experts in the field examine detailed methods involving proprotein convertases, the enzymes mediating this endoproteolysis, which reside within or cycle between the various compartments of the secretory pathway. Providing a timely assessment of impact of activation/inactivation endoproteolysis in the secretory pathway, the volume offers a broader perspective on the biochemistry of the PCSKs (proprotein convertases, subtilisin/kexin-type) by exploring structural and functional analogies with bacterial subtilisin and on the enzymology of endoproteolysis itself by describing the involvement in the process of non-PCSK-type such as cathepsin L. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Meticulous and up-to-date, Proprotein Convertases represents an instructive and useful reference book for all scientists interested in endoproteolytic activation and/or inactivation of secretory proproteins through limited proteolysis, for experts in the field and newcomers to it as well.
Автор: Geraldine Siegfried, Majid Khatib Название: Processing of VEGF-C and -D by the Proprotein Convertases: Importance in Angiogenesis, Lymphangiogenesis, and Tumorigenesis ISBN: 1615046100 ISBN-13(EAN): 9781615046102 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 61910.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Provides an overview of angiogenesis processes and discusses the importance of VEGF-C and VEGF-D precursors processing by the proprotein convertases during the activation of VEGFR-2 and VEGFR-3 receptors and the mediation of their functions during angiogenesis, lymphangiogenesis, and tumorigenesis.
Автор: Daniel Bassi, Jian Fu, Jirong Zhang Название: The Role of Proprotein Convertases in Animal Models of Skin Carcinogenesis ISBN: 1615045082 ISBN-13(EAN): 9781615045082 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 41580.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Many proprotein convertases (PC), especially furin and PACE4, are involved in pathological processes such as viral infection, inflammation, hypercholesterolemia, and cancer, and have been postulated as therapeutic targets for some of these diseases. In this chapter, we review mostly our work using animal models of squamous cancers that have been induced by chemical or UV carcinogenesis protocols to highlight the role of PCs in the development and progression of experimental tumors. After demonstrating in wild type mice the role of PACE4 in tumor progression as well as detecting the expression of PACE4 and furin in human non-melanoma skin cancers, we developed transgenic mice that over-express either PACE4 or furin in squamous epithelia, including the epidermis. This was accomplished by targeting the expression of the corresponding PC by using the promoter of the bovine keratin 5. Both K5-PACE4 and K5-Furin transgenic mice showed increased susceptibility to a two-stage carcinogenesis protocol of chemical carcinogenesis. Similar studies conducted in K5-PACE4 mice also showed an increased sensitivity to ultraviolet B radiation carcinogenesis. In most of these experiments, we were able to demonstrate that compared to the control wild type mice, the over-expression of the transgene in the epidermis increased the number of benign and malignant skin tumors and also had an effect on tumor progression as evidenced by the presence of less differentiated tumors and more frequent local and distant metastases in many of the transgenic lines. Interestingly, double transgenic mice in which PACE4 and furin are targeted to the epidermis did not show any additive effect, pointing to a probable in vivo overlap of functions at least in cutaneous tissues.The tumor-enhancing effects of PACE4 and furin further support their possible role as therapeutic targets. Furthermore, a proof of principle for PC inhibition as a therapeutic tool has been substantiated by an in vivo experiment in which the PC-inhibitor, decanoyl-RVKRchloromethylketone, was topically administrated to the skin of wild type and transgenic mice treated with chemical carcinogenesis protocols, resulting in a significant decrease of tumor development and progression.
Автор: Abdel-Majid Khatib Название: The Proprotein Convertases: Discovery, Characteristics and Link to Tumor Progression, Angiogenesis and Metastasis ISBN: 1615045368 ISBN-13(EAN): 9781615045365 Издательство: Mare Nostrum (Eurospan) Рейтинг: Цена: 41580.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Proprotein convertases (PCs) are a family of proteases including PC1, PC2, Furin, PC4, PACE4, PC5, and PC7. This title discusses a number of previous and recent findings on the PCs features, their implication in the regulation of multiple cellular functions that impact on the invasive/metastatic potential of cancer cells, and their clinical relevance in cancer patients.
Автор: Morgan E. Название: Biosynthesis in Insects: Advanced Edition ISBN: 1847558089 ISBN-13(EAN): 9781847558084 Издательство: Royal Society of Chemistry Рейтинг: Цена: 63360.00 T Наличие на складе: Невозможна поставка. Описание: This book provides a truly unique source for those working in the field, amalgamating previously fragmented information and recent exciting developments.
Автор: Noboru Motohashi Название: Anthocyanins: Structure, Biosynthesis & Health Benefits ISBN: 1622573293 ISBN-13(EAN): 9781622573295 Издательство: Nova Science Рейтинг: Цена: 211190.00 T Наличие на складе: Невозможна поставка. Описание: Dietary anthocyanins and flavonoids in vegetables, fruits and grain corms have their diverse functions to human dietary life. Topics discussed in this book include original findings and reviews such as their distributions, extractions, separations, characterization, stability, functions, biosynthesis pathway, the regulation of the biosynthetic enzymes by anthocyanins and flavonoids, food colours, and molecular biology such as a relationship of biomolecule with anthocyanins or flavonoids in vegetables and fruits. Kiwifruits, muscadine berries, color rices, Justicia species, strawberry, a Brazilian fruit of jabuticaba (Myrciaria cauliflora), jujube berry fruits, grapes, bilberries, taro as a vegetable and corn, and other fruits have been included in this book.
Автор: Sharma Swati, Kumar Ashok Название: Lignin: Biosynthesis and Transformation for Industrial Applications ISBN: 3030406628 ISBN-13(EAN): 9783030406622 Издательство: Springer Рейтинг: Цена: 158380.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: In the third part, chemical modification strategies and the preparation of composites as well as nano- and microparticles are discussed.The final part addresses the industrial application of lignin and its derivatives, as well as lignin materials.
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