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Optical Tweezers: Methods and Protocols, Gennerich Arne


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Автор: Gennerich Arne
Название:  Optical Tweezers: Methods and Protocols
ISBN: 9781493981946
Издательство: Springer
Классификация:
ISBN-10: 1493981943
Обложка/Формат: Paperback
Страницы: 555
Вес: 0.97 кг.
Дата издания: 23.06.2018
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 132 tables, color; 133 illustrations, color; 21 illustrations, black and white; xii, 555 p. 154 illus., 133 illus. in color.
Размер: 25.40 x 17.78 x 2.92 cm
Читательская аудитория: General (us: trade)
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание: The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells.

Programmed Morphogenesis: Methods and Protocols

Автор: Ebrahimkhani Mo R., Hislop Joshua
Название: Programmed Morphogenesis: Methods and Protocols
ISBN: 1071611739 ISBN-13(EAN): 9781071611739
Издательство: Springer
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Supramolecules in Drug Discovery and Drug Delivery: Methods and Protocols

Автор: Mavromoustakos Thomas, Tzakos Andreas G., Durdagi Serdar
Название: Supramolecules in Drug Discovery and Drug Delivery: Methods and Protocols
ISBN: 107160919X ISBN-13(EAN): 9781071609194
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
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Описание: This detailed book aims to provide readers with critical information to accomplish the synthesis of nanosystems for the purpose of supramolecular entities complexing with drugs, targeted drug delivery system characterization, as well as the study of the physical-chemical interactions that govern the stability and properties of these systems.

PCR: Methods and Protocols

Автор: Domingues Lucнlia
Название: PCR: Methods and Protocols
ISBN: 1493983814 ISBN-13(EAN): 9781493983810
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 1. In silico PCR Tools for a Fast Primer, Probe and Advanced Searching

Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, and Kabyl Zhambakin

2. Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis

Ruslan Kalendar and Timofey V. Tselykh

3. Long Fragment Polymerase Chain Reaction

Eng Wee Chua, Simran Maggo, and Martin A. Kennedy

4. Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR

Maria Jorge Campos and Alberto Quesada

5. Inverse PCR for Point Mutation Introduction

Diogo Silva, Gustavo Santos, Mбrio Barroca, and Tony Collins

6. Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR

Tatiana Q. Aguiar, Carla Oliveira, and Lucнlia Domingues

7. A Novel Platform for High-throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli

Ana Filipa Sequeira, Joana L.A. Brбs, Vвnia O. Fernandes, Catarina I.P.D. Guerreiro, Renaud Vincentelli, and Carlos M.G.A. Fontes

Flбvio Azevedo, Humberto Pereira, and Bjцrn Johansson

9. CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water

Luc Bissonnette, Andrйe F. Maheux, and Michel G. Bergeron

10. Multiplex Detection Of Food-borne Pathogen

Germбn Villamizar-Rodrнguez and Felipe Lombу

11. Fast Real-time PCR for the Detection of Crustacean Allergens in Foods

Francisco J. Santaclara and Montserrat Espiсeira

12. Fast Real-time PCR Method for the Detection of Soy in Foods

Montserrat Espiсeira and Francisco J. Santaclara

13. RAPD/SCAR Approaches for Identification of Adulterant's Breed's Milk in Dairy Products

Joana Cunha and Lucнlia Domingues

14. Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers

Farina Mujeeb, Preeti Bajpai, Neelam Pathak, and Smita Rastogu Verma

15. PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism

Laura Vilarinho and Cйlia Nogueira

16. Harnessing the Power of PCR-dependent Molecular Fingerprinting Methods for Understanding Structure and Function in Microbial Communities

S.S. Phadke, A.F. Salvador, J.I. Alves, O. Bretschger, M.M. Alves, and M.A. Pereira

17. PCR in Metagenomics

Sarita G Bhat and Tina K. J.

18. Arbitrarily Primed PCR for Comparison of Metagenomes and Extracting useful loci from them

Leigh Burgoyne, Lin Y Koh, and David Catcheside


Optical Tweezers

Автор: Arne Gennerich
Название: Optical Tweezers
ISBN: 1493964194 ISBN-13(EAN): 9781493964192
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Optical Tweezers: Methods and Protocols aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines.

Biosensors and Biodetection: Methods and Protocols Volume 1: Optical-Based Detectors

Автор: Rasooly Avraham, Prickril Ben
Название: Biosensors and Biodetection: Methods and Protocols Volume 1: Optical-Based Detectors
ISBN: 1493983164 ISBN-13(EAN): 9781493983162
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

1. Localized Surface Plasmon Resonance (LSPR)-Coupled Fiber-Optic Nanoprobe for the Detection of Protein Biomarkers

Jianjun Wei, Zheng Zeng, and Yongbin Lin

2. Ultra-Sensitive Surface Plasmon Resonance Detection by Colocalized 3D Plasmonic Nanogap Array

Wonju Lee, Taehwang Son, Changhun Lee, Yongjin Oh, and Donghyun Kim

3. Two-dimensional Surface Plasmon Resonance Imaging (2D-SPR) System for Cellular Analysis

Tanveer Ahmad Mir and Hiroaki Shinohara

4. Immunosensing with Near-infrared Plasmonic Optical Fiber Gratings

Christophe Caucheteur, Clotilde Ribaut, Viera Malachovska, and Ruddy Wattiez

5. Biosensing Based on Magneto-Optical Surface Plasmon Resonance

Sorin David, Cristina Polonschii, Mihaela Gheorghiu, Dumitru Bratu, and Eugen Gheorghiu

6. Nanoplasmonic Biosensor Using Localized Surface Plasmon Resonance Spectroscopy for Biochemical Detection

Diming Zhang, Qian Zhang, Yanli Lu, Yao Yao, Shuang Li, and Qingjun Liu

7. Plasmonics Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles

Changwon Lee, Peng Wang, Marsha A. Gaston, Alison A. Weiss, and Peng Zhang

8. MicroRNA Biosensing with Two-dimensional Surface Plasmon Resonance Imaging

Ho Pui Ho, Fong Chuen Loo, Shu Yuen Wu, Dayong Gu, Ken-Tye Yong, and Siu Kai Kong

9. Gold Nanorod Array Biochip for Label-free, Multiplexed Biological Detection

Zhong Mei, Yanyan Wang, and Liang Tang

10. Resonant Waveguide Grating Imager for Single Cell Monitoring of the Invasion of Spheroidal Cancer Cells through Matrigel

Nicole K. Febles, Siddarth Chandrasekaran, and Ye Fang

11. Label-Free Biosensors Based on Bimodal Waveguide (BiMW) Interferometers

Sonia Herranz, Adriбn Fernбndez Gavela, and Laura M. Lechuga

12. DNA-Directed Antibody Immobilization for Robust Protein Microarrays: Application to Single Particle Detection 'DNA-directed Antibody Immobilization'

Nese Lortlar Ьnlь, Fulya Ekiz Kanik, Elif Seymour, John H. Connor, and M. Selim Ьnlь

13. Reflectometric Interference Spectroscopy

Guenther Proll, Goran Markovic, Peter Fechner, Florian Proell, and Guenter Gauglitz

14. Hypermulticolor Detector for Quantum-antibody Based Concurrent Detection of Intracellular Markers for HIV Diagnosis

Annie Agnes Suganya and Joon Myong Song

15. Low Cost Charged-coupled Device (CCD) Based Detectors for Shiga Toxins Activity Analysis

Reuven Rasooly, Ben Prickril, Hugh A. Bruck, and Avraham Rasooly

16. Smartphone-enabled Detection Strategies for Portable PCR-based Diagnostics

Aashish Priye and Victor M. Ugaz

17. Streak Imaging Flow Cytometer for Rare Cell Analysis

Joshua Balsam, Hugh Alan Bruck, Miguel Ossandon, Ben Prickril, and Avraham Rasooly

18. Rapid Detection of Microbial Contamination Using a Microfluidic Device

Mustafa Al-Adhami, Dagmawi Tilahun, Govind Rao, Chandrasekhar Gurramkonda, and Yordan Kostov

19. Resonance Energy Transfer-Based Nucleic Acid Hybridization Assays on Paper-Based Platforms Using Emissive Nanoparticles as Donors

Samer Doughan, M. Omair Noor, Yi Han, and Ulrich J. Krull

20. Enhanced Performance of Colorimetric Biosensing on Paper Microfluidic Platforms through Chemical Modification and Incorporation of Nanoparticles

Ellen Flбvia Moreira Gabriel, Paulo T. Garcia, Elizabeth Evans, Thiago Miguel Garcia Cardoso, Carlos D.

PCR Methods and Protocols

Автор: Domingues, Lucilia
Название: PCR Methods and Protocols
ISBN: 1493970593 ISBN-13(EAN): 9781493970599
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 1. In silico PCR Tools for a Fast Primer, Probe and Advanced Searching

Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, and Kabyl Zhambakin

2. Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis

Ruslan Kalendar and Timofey V. Tselykh

3. Long Fragment Polymerase Chain Reaction

Eng Wee Chua, Simran Maggo, and Martin A. Kennedy

4. Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR

Maria Jorge Campos and Alberto Quesada

5. Inverse PCR for Point Mutation Introduction

Diogo Silva, Gustavo Santos, Mбrio Barroca, and Tony Collins

6. Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR

Tatiana Q. Aguiar, Carla Oliveira, and Lucнlia Domingues

7. A Novel Platform for High-throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli

Ana Filipa Sequeira, Joana L.A. Brбs, Vвnia O. Fernandes, Catarina I.P.D. Guerreiro, Renaud Vincentelli, and Carlos M.G.A. Fontes

Flбvio Azevedo, Humberto Pereira, and Bjцrn Johansson

9. CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water

Luc Bissonnette, Andrйe F. Maheux, and Michel G. Bergeron

10. Multiplex Detection Of Food-borne Pathogen

Germбn Villamizar-Rodrнguez and Felipe Lombу

11. Fast Real-time PCR for the Detection of Crustacean Allergens in Foods

Francisco J. Santaclara and Montserrat Espiсeira

12. Fast Real-time PCR Method for the Detection of Soy in Foods

Montserrat Espiсeira and Francisco J. Santaclara

13. RAPD/SCAR Approaches for Identification of Adulterant's Breed's Milk in Dairy Products

Joana Cunha and Lucнlia Domingues

14. Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers

Farina Mujeeb, Preeti Bajpai, Neelam Pathak, and Smita Rastogu Verma

15. PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism

Laura Vilarinho and Cйlia Nogueira

16. Harnessing the Power of PCR-dependent Molecular Fingerprinting Methods for Understanding Structure and Function in Microbial Communities

S.S. Phadke, A.F. Salvador, J.I. Alves, O. Bretschger, M.M. Alves, and M.A. Pereira

17. PCR in Metagenomics

Sarita G Bhat and Tina K. J.

18. Arbitrarily Primed PCR for Comparison of Metagenomes and Extracting useful loci from them

Leigh Burgoyne, Lin Y Koh, and David Catcheside


Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

Автор: Marx
Название: Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols
ISBN: 1617377260 ISBN-13(EAN): 9781617377266
Издательство: Springer
Рейтинг:
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals.

Nanotechnology for Nucleic Acid Delivery: Methods and Protocols

Автор: Ogris Manfred, Oupicky David
Название: Nanotechnology for Nucleic Acid Delivery: Methods and Protocols
ISBN: 1493959328 ISBN-13(EAN): 9781493959327
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Methods in Molecular Biology (TM) series book covers nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Includes materials and reagents, protocols, notes on troubleshooting and more.

Synthetic Gene Circuits: Methods and Protocols

Автор: Menolascina Filippo
Название: Synthetic Gene Circuits: Methods and Protocols
ISBN: 1071610317 ISBN-13(EAN): 9781071610312
Издательство: Springer
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Cryoem: Methods and Protocols

Автор: Gonen Tamir, Nannenga Brent L.
Название: Cryoem: Methods and Protocols
ISBN: 1071609653 ISBN-13(EAN): 9781071609651
Издательство: Springer
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Cell Microencapsulation: Methods and Protocols

Автор: Opara Emmanuel C.
Название: Cell Microencapsulation: Methods and Protocols
ISBN: 1493981773 ISBN-13(EAN): 9781493981779
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

Part I Introduction

1. Historical Perspectives and Current Challenges in Cell Microencapsulation
Paul de Vos

2. Applications of Cell Microencapsulation
Emmanuel C. Opara

Part II Approaches to Cell Microencapsulation

3. Cell Microencapsulation:
Dripping Methods
A. Bidoret, E. Martins, B. Poncelet De Smet, and D. Poncelet

4. Field Effect Microparticle Generation for Cell Microencapsulation
Brend Ray-Sea Hsu and Shin-Huei Fu

5. Microfluidic Approach to Cell Microencapsulation
Varna Sharma, Michael Hunckler, Melur K. Ramasubramanian, Emmanuel C. Opara, and Kalyan C. Katuri

Part III Biomaterials and Enabling Technologies in Cell Microencapsulation

6. Polymeric Materials for Cell Microencapsulation
A. Aijaz, D. Perera, and R. Olabisi
William F. Kendall and Emmanuel C. Opara

8. Determination of the Mechanical Strength of Microcapsules
Marcus D. Darrabie and Emmanuel C. Opara

9. The Diffusive Properties of Hydrogel Microcapsules for Cell Encapsulation
D.M. Lavin, B.E. Bintz, and C.G. Thanos

10. Methods for Incorporating Oxygen Generating Biomaterials into Cell Culture and Microcapsule Systems
John Patrick McQuilling and Emmanuel C. Opara

11. Non-Invasive Tracking of Alginate-Microencapsulated Cells
Genaro A. Paredes-Juarez, Brad P. Barnett, and Jeff W.M. Bulte

12. Retrieval of Microencapsulated Islet Grafts for Post-Transplant Evaluation
John Patrick McQuilling, Sivanandane Sittadjody, Rajesh Pareta, Samuel Pendergraft, Clancy J. Clark, Alan C. Farney, and Emmanuel C. Opara

Part IV Isolation of Cells for Microencapsulation

13. A Method of Porcine Pancreatic Islet Isolation for Microencapsulation
William F. Kendall and Emmanuel C. Opara

14: Selective Osmotic Shock (SOS)-Based Islet Isolation for Microencapsulation
Kevin Enck, John P. McQuilling, Giuseppe Orlando, Riccardo Tamburrini, Sittadjody Sivanandane, and Emmanuel C. Opara

15. Preparation and Characterization of Alginate-Chitosan Microcapsule for Hepatocyte Culture
Lamjuan Li, Yanhong Zhang, and Xiaoping Pan

16. Use of Flow Focusing Technique for Microencapsulation of Myoblasts
J. Ciriza, L. Saenz del Burgo, RM Hernandez, G. Orive, and JL Pedraz

17. Alginate Microbeads for Cell and Protein Delivery
Sami I. Somo, Omaditya Khanna, and Eric M. Brey

18. Compartmentalization of Two Cell Types in Multi-Layered Alginate Microcapsules
Sivanandane Sittadjody, Justin M. Saul, and Emmanuel C. Opara

19. Primary Choroid Plexus Tissue for Use in Cellular Therapy
M.A. Sandrof and C.G. Thanos

20. Microencapsulation of Stem Cells for Therapy
Shirae K. Leslie, Ramsey C. Kinney, Zvi Schwartz, and Barbara D. Boyan

21. Microencapsulated Cells for Cancer Therapy
L. Saenz del Burgo, J. Ciriza, RM Hernбndez, G. Orive, and JL Pedraz

22. Microencapsulation of Bacterial Cells by Emulsion Technique for Probiotic Application
Surajit Mandal and Subrota Hati

Part V Cell Microencapsulation in Clinical Applications

23. Microencapsulation of Islets for the Treatment of Type 1 Diabetes Mellitus (T1D)
Riccardo Calafiore, Giuseppe Basta, and Pia Montanucci


High Throughput Screening: Methods and Protocols

Автор: Janzen William P.
Название: High Throughput Screening: Methods and Protocols
ISBN: 1493981137 ISBN-13(EAN): 9781493981137
Издательство: Springer
Рейтинг:
Цена: 106010.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

1. Design and Implementation of High Throughput Screening Assays
David J. Powell, Robert P. Hertsberg, and Ricardo Macarrόn

2. Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method
Thomas V. Riera, Tim J. Wigle, and Robert A. Copeland

3. High Throughput Screening Using Mass Spectrometry within Drug Discovery
Mattias Rohman and Jonathan Wingfield

4. Structure-Based Virtual Screening of Commercially Available Compound Libraries
Dmitri Kireev

5. AlphaScreen-Based Assays: Ultra-High Throughput Screening for Small Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions
Adam Yasgar, Ajit Jadhav, Anton Simeonov, and Nathan P. Coussens

6. Instrument Quality Control
Chatura Jayakody and Emily A. Hull-Ryde

7. Application of Fluorescence Polarization in HTS AssaysXinyi Huang and Ann Aulabaugh

8. Time Resolved Fluorescence Assays
Chen-Ting Ma and Eduard A. Sergienko

9. Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays
Peter Drueckes

10. Screening for Inhibitors of Kinase Autophosphorylation
Bianca Heedmann and Martin Klumpp

11. A Fluorescence-Based High Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
Thomas M. Smith, Daryl L. Richie, Jianshi Tao

12. Mycobacterium tuberculosis High Throughput Screening
E. Lucile White, Nichole A. Tower, and Lynn Rasmussen

13. Identification of State-Dependent Blockers for Voltage Gated Calcium Channels Using a FLIPR Based Assay
Alberto di Silvio

14. A Luciferase Reporter Gene System for High Throughput Screening of γ-Globin Gene Activators
Wensheng Xie, Robert Silvers, Michael Ouellette, Zining Wu, Quinn Lu, Hu Li, Kathleen Gallagher, Kathy Johnson, and Monica Montoute

15. A High Throughput Flow Cytometry Assay for Identification of Inhibitors of 3', 5'-Cyclic Adenosine Monophosphate Efflux
Dominique Perez, Peter C. Simons, Yelena Smagley, Larry A. Sklar, and Alexandre Chigaev

16. High Throughput Cell Toxicity Assays
David Murray, Lisa McWilliams, and Mark Wigglesworth

17. BRETn: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells
Xiu-Lei Mo and Haian Fu

18. Application of Imaging-Based Assays in Microplate Formats for High Content Screening
Adam I. Fogel, Scott E. Martin, and Samuel A. Hasson



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