Cryoem: Methods and Protocols, Gonen Tamir, Nannenga Brent L.

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 38730.00 T
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 13330.00 T
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Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Kwok Pui-Yan
Название: Single Nucleotide Polymorphisms / Methods and Protocols
ISBN: 0896039684 ISBN-13(EAN): 9780896039681
Издательство: Springer
Цена: 38730.00 T
Наличие на складе: Есть
Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Автор: Kister Alexander E.
Название: Protein Supersecondary Structures: Methods and Protocols
ISBN: 1493991604 ISBN-13(EAN): 9781493991600
Издательство: Springer
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Цена: 139750.00 T
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Автор: Qi Yiping
Название: Plant Genome Editing with Crispr Systems: Methods and Protocols
ISBN: 1493989901 ISBN-13(EAN): 9781493989904
Издательство: Springer
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Цена: 214280.00 T
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Описание: This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Автор: Khalaf Yasmin
Название: DNA and RNA Extraction: Methods and Protocols
ISBN: 3659495034 ISBN-13(EAN): 9783659495038
Издательство: Неизвестно
Цена: 86330.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.

Автор: Israel
Название: Verbal Protocols in Literacy Research
ISBN: 1138084816 ISBN-13(EAN): 9781138084810
Издательство: Taylor&Francis
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Цена: 42870.00 T
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Описание: This volume offers an updated analysis of the methodology of reading and reading research since 1995, when the landmark book Verbal Protocols of Reading: The Nature of Constructively Responsive Reading by Michael Pressley and Peter Afflerbach was published. It offers a thorough cross-analysis of the conscious processes experienced during reading, the structure of reading comprehension, and its application to more current initiatives such as Common Core State Standards and Response to Intervention. It also provides a detailed analysis of Constructively Responsive Reading through relevant online self-report studies in reading and reading comprehension behavior. It is a fresh and comprehensive volume that speaks not only to reading researchers, but to literacy teachers at all levels.

Автор: Wendy A. Harwood
Название: Barley: Methods and Protocols
ISBN: 1493989421 ISBN-13(EAN): 9781493989423
Издательство: Springer
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Цена: 186330.00 T
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Описание: This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Автор: Streit Wolfgang R., Daniel Rolf
Название: Metagenomics: Methods and Protocols
ISBN: 1493982745 ISBN-13(EAN): 9781493982745
Издательство: Springer
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Цена: 83850.00 T
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Описание: This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. Metagenomics: Methods and Protocols, Second Edition also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Metagenomics: Methods and Protocols, Second Edition is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory.

Автор: Perez Daniel R.
Название: Reverse Genetics of RNA Viruses: Methods and Protocols
ISBN: 1493983539 ISBN-13(EAN): 9781493983537
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
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Описание: This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Автор: Theodoridis Georgios A., Gika Helen G., Wilson Ian D.
Название: Metabolic Profiling: Methods and Protocols
ISBN: 1493976427 ISBN-13(EAN): 9781493976423
Издательство: Springer
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Цена: 88500.00 T
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Описание:

Preface...

Table of Contents...

Contributing Authors...

Part I Fundamentals

1. Metabolic Profiling: Topic, Challenges, Perspective

Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson

Автор: Ivan A. Vorobjev
Название: Cellular Heterogeneity Methods and Protocols
ISBN: 1493976796 ISBN-13(EAN): 9781493976799
Издательство: Springer
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Цена: 102480.00 T
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Описание: This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts.