Автор: Horowitz Paul Название: The art of electronics The x Chapters ISBN: 1108499945 ISBN-13(EAN): 9781108499941 Издательство: Cambridge Academ Рейтинг: Цена: 59130.00 T Наличие на складе: Заказано в издательстве. Описание: The x-Chapters is the missing pieces of The Art of Electronics, to be used either as its complement, or as a direct route to exploring some of the most exciting and oft-overlooked topics in advanced electronic engineering. Students, researchers and practitioners will find here techniques and circuits that are unavailable elsewhere.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 38730.00 T Наличие на складе: Есть Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 13330.00 T Наличие на складе: Есть Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Ishikawa Kiyotake Название: Cardiac Gene Therapy: Methods and Protocols ISBN: 1493982435 ISBN-13(EAN): 9781493982431 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets.
Автор: Soucek Laura, Whitfield Jonathan Название: The Myc Gene: Methods and Protocols ISBN: 1071614754 ISBN-13(EAN): 9781071614754 Издательство: Springer Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: 1. An "-omycs" Toolbox to Work with MYC
Jonathan R. Whitfieldand Laura Soucek
2. Methods of Expression, Purification and Preparation of the c-Myc b-HLH-LZ for its Biophysical Characterization Patrick Delattre, Martin Montagne, and Pierre Lavigne
3. Biophysical and Structural Methods to Study the bHLHzip Region of Human c-MYC
Giovanna Zinzalla
4. Identifying and Validating MYC: protein Interactors in Pursuit of Novel anti-MYC Therapies
Diana Resetca, Alannah S. MacDonald, Tristan M. G. Kenney, Yong Wei, Cheryl H. Arrowsmith, Brian Raught, and Linda Z. Penn
5. Detection of Post-Translational Modifications on MYC
Colin J. Daniel, Xiao-Xin Sun, Yingxiao Chen, Xiaoli Zhang, Mu-Shui Dai, and Rosalie C. Sears
6. MYC Analysis in Cancer and Evolution
Markus Hartl and Klaus Bister
7. Genome-wide analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing
Rene Jackstadt, Markus Kaller, Antje Menssen, and Heiko Hermeking
8. Analysis of Myc Chromatin Binding by Calibrated ChIP-seq Approach
Donald P. Cameron, Vladislav Kuzin, and Laura Baranello
9. A High-Throughput Chromatin Immunoprecipitation Sequencing Approach to Study the Role of MYC on the Epigenetic Landscape
Luca Fagnocchiand Alessio Zippo
10. Methods for Determining Myc-induced Apoptosis
Dan Lu, Catherine Wilson, and Trevor D. Littlewood
11. Measuring MYC-mediated Metabolism in Tumorigenesis
Hsin-Yao Tang, Aaron R. Goldman, Xue Zhang, David W. Speicher, and Chi V. Dang
12. Methods to study Myc-regulated Cellular Senescence - An Update
Fan Zhang, Wesam Bazzar, Mohammad Alzrigat, and Lars-Gunnar Larsson
13. Examining Myc-Dependent Translation Changes in Cellular Homeostasis and Cancer
Joanna R. Kovalski, Yichen Xu, and Davide Ruggero
14. A Versatile in vivo System to Study Myc in Cell Reprogramming
Elena Senis, Lluc Mosteiro, Dirk Grimm, and Maria Abad
15. Using Flow Cytometry to Study Myc's Role in Shaping the Tumor Immune Microenvironment
Sнlvia Casacuberta-Serra
16. Generation of a Tetracycline Regulated Mouse Model of MYC-Induced
T-Cell Acute Lymphoblastic Leukemia
Wadie D. Mahauad-Fernandez, Kavya Rakhra, and Dean W. Felsher
17. Chromogenic inSitu Hybridization (CISH) as a Method for Detection of C-Myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue: An Update
Natasa Todorovic-Rakovic
18. MYC Copy Number Detection in Clinical Samples using a Digital DNA-Hybridization and Detection Method
Zighereda Ogbah, Francesco Mattia Mancuso, and Ana Vivancos
19. Cell-Based Methods for the Identification of Myc-Inhibitory Small Molecules
Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov
Автор: Minczuk Michal, Rorbach Joanna Название: Mitochondrial Gene Expression: Methods and Protocols ISBN: 1071608339 ISBN-13(EAN): 9781071608333 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Authoritative and cutting-edge, Mitochondrial Gene Expression: Methods and Protocols aims to provide complementary approaches and practical guidelines. Chapter 7 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Автор: Bandyopadhyay Anindya, Thilmony Roger Название: Rice Genome Engineering and Gene Editing: Methods and Protocols ISBN: 1071610678 ISBN-13(EAN): 9781071610671 Издательство: Springer Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.
Автор: Kaufmann Kerstin, Mueller-Roeber Bernd Название: Plant Gene Regulatory Networks: Methods and Protocols ISBN: 149398392X ISBN-13(EAN): 9781493983926 Издательство: Springer Рейтинг: Цена: 149060.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Preface... Table of Contents...
Contributing Authors...
1. From Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants
Kerstin Kaufmann and Dijun Chen
Part I Experimental Approaches to Study Plant Gene-Regulatory Networks
2. Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis Bennett Thomson, Emmanuelle Graciet, and Frank Wellmer
3. Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting Daniel Slane and Martin Bayer
4. Characterization of Cell Type-Specific DNA Binding Sites of Plant Transcription Factors using Chromatin Immunoprecipitation On Sun Lau
5. Yeast One and Two Hybrid High-Throughput Screenings using Arrayed Libraries Rocнo Sбnchez-Montesino and Luis Oсate-Sбnchez
6.SELEX-Seq: A Method to Determine DNA-Binding Specificities of Plant Transcription Factors Cezary Smaczniak, Gerco C. Angenent and Kerstin Kaufmann
7. Analysis of a Plant Transcriptional Regulatory Network using Transient Expression Systems Sara Dнaz-Triviсo, Yuchen Long, Ben Scheres, and Ikram Blilou
8.Analysis of In Vivo Chromatin and Protein Interactions of Arabidopsis Transcript Elongation Factors Alexander Pfab, Wojciech Antosz, Philipp Holzinger, Astrid Bruckmann, Joachim Griesenbeck, and Klaus D. Grasser
9.Characterization of Mediator Complex and its Associated Proteins from Rice Subhasis Samanta and Jitendra Kumar Thakur
10. DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing Sergei A. Filichkin and Molly Megraw
11. In Situ Hi-C Library Preparation for Plants to Study their Three-Dimensional Chromatin Interactions on a Genome-Wide Scale Chang Liu
12. Multiplexed Transcriptional Activation or Repression in Plants using CRISPR-dCas9 Based Systems Levi G. Lowder, Joseph W. Paul III, and Yiping Qi
13. Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants Tom Schreiber and Alain Tissier
Part II Computational Approaches to Study Plant Gene-Regulatory Networks
14.Design of Knowledge Bases for Plant Gene Regulatory Networks Eric Mukundi, Fabio Gomez-Cano, Wilberforce Zachary Ouma, and Erich Grotewold
15. AraNet: A Network Biology Server for Arabidopsis thaliana and Other Non-Model Plant Species Tak Lee and Insuk Lee
16. Integration of Genome-Wide TF Binding and Gene Expression Data to Characterize Gene Regulatory Networks in Plant Development Dijun Chen and Kerstin Kaufmann
17. Predicting Transcription Factor-Binding Sites and their Cognate Transcription Factors using Gene Expression Data Chun-Ping Yu and Wen-Hsiung Li
18. Computational Approaches to Study Gene Regulatory Networks Nooshin Omranian and Zoran Nikoloski
19. Boolean Dynamic Modeling Approaches to Study Plant Gene Regulatory Networks: Integration, Validation and Prediction J. Davila-Velderrain, J.C. Martinez-Garcia, and R.E. Alvarez Buylla
20. ODE-Based Modeling of Complex Regulatory Circuits Daniel D. Seaton
21. Inferring Gene Regulatory Networks in the Arabidopsis Root using a Dynamic Bayesian Network Approach Maria Ang
Автор: Grutzmann Robert, Pilarsky Christian Название: Cancer Gene Profiling: Methods and Protocols ISBN: 1493979973 ISBN-13(EAN): 9781493979974 Издательство: Springer Рейтинг: Цена: 106650.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Factors Affecting the Use of Human Tissues in Biomedical Research: Implications in the Design and Operation of a Biorepository Daniel S. Atherton, Katherine C. Sexton, Dennis Otali, Walter C. Bell, and William E. Grizzle
2. Tissue Microdissection Anja Rabien and Glen Kristiansen
3. Tissue Microarrays Ana-Maria Dancau, Ronald Simon, Martina Mirlacher, and Guido Sauter
4. Gene Expression Analysis in the Age of Mass Sequencing: An Introduction Christian Pilarsky, Lahiri Kanth Nanduri, and Janine Roy
5. Quantitative DNA Methylation in Cancer Ole Ammerpohl, Andrea Haake, Julia Kolarova, and Reiner Siebert
6. Single Nucleotide Polymorphism to Associate Cancer Risk Victoria Shaw, Katie Bullock, and William Greenhalf
7: Exosomes in Cancer Disease Margot Zцller
8: MicroRNAs (miRNAs) Profiling Lu Gao and Feng Jiang
9. Cancer Gene Profiling for Response Prediction B. Michael Ghadimi and Peter Jo
10. Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment Sanjay Tiwari, Ole Ammerpohl, and Holger Kalthoff
11. Application of Proteomics in Cancer Biomarker Discovery: GeLC-MS/MS Pedro R. Cutillas and Tatjana Crnogorac-Jurcevic
12. Meta-Analysis of Cancer Gene Profiling Data Janine Roy, Christof Winter, and Michael Schroeder
13. Reproducible, Scalable Fusion Gene Detection from RNA-Seq Vladan Arsenijevic and Brandi N. Davis-Dusenbery
14. Transcriptome Sequencing for the Detection of Chimeric Transcripts Hsueh-Ting Chu
Автор: Burgess-Brown Nicola A. Название: Heterologous Gene Expression in E.Coli: Methods and Protocols ISBN: 1493983288 ISBN-13(EAN): 9781493983285 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E.
Автор: Hehl Reinhard Название: Plant Synthetic Promoters: Methods and Protocols ISBN: 1493981862 ISBN-13(EAN): 9781493981861 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.
Автор: Stein Viktor Название: Synthetic Protein Switches: Methods and Protocols ISBN: 1493983458 ISBN-13(EAN): 9781493983452 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, ss-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches.
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