Peroxisomes: Methods and Protocols, Schrader Michael
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 13330.00 T Наличие на складе: Есть Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 38730.00 T Наличие на складе: Есть Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Goswami Ritobrata Название: Th9 Cells: Methods and Protocols ISBN: 1493983261 ISBN-13(EAN): 9781493983261 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model.
5. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay
Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Lцhr
6. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
Xiaoxing Cheng and Rui Jian
7. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells
Soizik Berlivet, Isabelle Hmitou, Hйlиne Picaud, and Matthieu Gйrard
8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
Takayuki Manabe and Akio Wanaka
9. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells
Melanie I. Trombly and Xiaozhong Wang
10. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells
Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu
11. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells
Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang
12. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry
Mario Lцhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, Gyцrgy A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann
13. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells
Amelia J. Helms, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables
14. In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation
Veit Riechmann
15. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans
Myon-Hee Lee and Dong Suk Yoon
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Автор: Higgins Paul J. Название: Transcription Factors: Methods and Protocols ISBN: 1493957155 ISBN-13(EAN): 9781493957156 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Here, leading researchers describe the latest techniques for investigating the mechanisms underlying transcription factor nuclear-cytoplasmic trafficking, as well as the functional impact of post-translational modifications on transcription factor function.
Автор: Luis A. del R?o Название: Peroxisomes and their Key Role in Cellular Signaling and Metabolism ISBN: 9401782903 ISBN-13(EAN): 9789401782906 Издательство: Springer Рейтинг: Цена: 130590.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Peroxisomes are a class of ubiquitous and dynamic single membrane-bounded cell organelles, devoid of DNA, with an essentially oxidative type of metabolism.
Автор: Norbert Latruffe; Maurice Bugaut Название: Peroxisomes ISBN: 3642878091 ISBN-13(EAN): 9783642878091 Издательство: Springer Рейтинг: Цена: 46570.00 T Наличие на складе: Есть у поставщика Поставка под заказ.
Автор: Tsuneo Imanaka; Nobuyuki Shimozawa Название: Peroxisomes: Biogenesis, Function, and Role in Human Disease ISBN: 9811511683 ISBN-13(EAN): 9789811511684 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This book provides readers with a comprehensive overview of peroxisomes and their role in human diseases. It starts by describing the history of peroxisome research and then examines in detail the current understanding of the biogenesis and function of peroxisomes. It then focuses on peroxisomal disorders and the involvement of peroxisomes in cancer and age-related diseases, discussing in detail the use of model organisms to elucidate the pathogenesis of peroxisomal disorders and the physiological importance of peroxisomal proteins. Further, the book examines diagnostic and therapeutic strategies in peroxisomal disorders as well as significant recent advances. Lastly, it addresses various topics in peroxisome research, including the isolation of peroxisomes from mammalian tissues and cells, the structural biology of peroxisomal proteins, the lipidomics of peroxisomal disorders, the value of exome sequencing, and neuropsychological testing in X-linked adrenoleukodystrophy. Given its scope, the book is a valuable resource for postgraduate students and researchers in the life sciences and clinicians in the fields of internal medicine, pediatrics, and neurology.
Автор: Luis A. del R?o; Michael Schrader Название: Proteomics of Peroxisomes ISBN: 9811322325 ISBN-13(EAN): 9789811322327 Издательство: Springer Рейтинг: Цена: 232910.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This new edited volume in the Springer Subcellular Biochemistry Series presents a comprehensive, state-of-the-art overview of the proteomics of peroxisomes derived from mammalian, Drosophila, fungal, and plant origin, and contains contributions from leading experts in the field. The development of sensitive proteomics and mass spectrometry technologies, combined with bioinformatics approaches now allow the identification of low-abundance and transient peroxisomal proteins and permits to identify the complete proteome of peroxisomes, with the consequent increase of our knowledge of the metabolic and regulatory networks of these important cellular organelles. The book lines-up with these developments and is organized in four sections including: (i) mass spectrometry-based organelle proteomics; (ii) prediction of peroxisomal proteomes; (iii) analysis of peroxisome proteome interaction networks; and (iv) peroxisomes in relation to other subcellular compartments. The editor Luis A. del R?o is Professor ad honorem of the Spanish National Research Council (CSIC) in the Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry and Cell & Molecular Biology of Plants, at the Estaci?n Experimental del Zaid?n, Granada, Spain. Del R?o’s research group focuses on the metabolism of reactive oxygen species (ROS), reactive nitrogen species (RNS) and antioxidants in plant peroxisomes, and the ROS- and RNS-dependent role of peroxisomes in plant cell signalling. The editor Michael Schrader is Professor of Cell Biology & Cytopathology in the Department of Biosciences at the University of Exeter, UK. Using mammalian peroxisomes as model organelles, Prof. Schrader and his team aim to unravel the molecular machinery and signalling pathways that mediate and regulate the formation, dynamics and abundance of these medically relevant cellular compartments.
Автор: Luis A. del R?o; Michael Schrader Название: Proteomics of Peroxisomes ISBN: 9811347530 ISBN-13(EAN): 9789811347535 Издательство: Springer Рейтинг: Цена: 232910.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This new edited volume in the Springer Subcellular Biochemistry Series presents a comprehensive, state-of-the-art overview of the proteomics of peroxisomes derived from mammalian, Drosophila, fungal, and plant origin, and contains contributions from leading experts in the field. The development of sensitive proteomics and mass spectrometry technologies, combined with bioinformatics approaches now allow the identification of low-abundance and transient peroxisomal proteins and permits to identify the complete proteome of peroxisomes, with the consequent increase of our knowledge of the metabolic and regulatory networks of these important cellular organelles. The book lines-up with these developments and is organized in four sections including: (i) mass spectrometry-based organelle proteomics; (ii) prediction of peroxisomal proteomes; (iii) analysis of peroxisome proteome interaction networks; and (iv) peroxisomes in relation to other subcellular compartments. The editor Luis A. del R?o is Professor ad honorem of the Spanish National Research Council (CSIC) in the Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry and Cell & Molecular Biology of Plants, at the Estaci?n Experimental del Zaid?n, Granada, Spain. Del R?o’s research group focuses on the metabolism of reactive oxygen species (ROS), reactive nitrogen species (RNS) and antioxidants in plant peroxisomes, and the ROS- and RNS-dependent role of peroxisomes in plant cell signalling. The editor Michael Schrader is Professor of Cell Biology & Cytopathology in the Department of Biosciences at the University of Exeter, UK. Using mammalian peroxisomes as model organelles, Prof. Schrader and his team aim to unravel the molecular machinery and signalling pathways that mediate and regulate the formation, dynamics and abundance of these medically relevant cellular compartments.
Автор: H. Dariush Fahimi; Helmut Sies Название: Peroxisomes in Biology and Medicine ISBN: 3642713270 ISBN-13(EAN): 9783642713279 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Peroxisomes (microbodies) have a long evolutionary lifespan, but in cell biology they are still young. It is gratifying to see that most recent advances in our knowledge of peroxisomes come from quite different disciplines, ranging from molecular biology and cell biology to clinical research. The identification in terms of cDNA sequences of enzymes, the localization in terms of immuno-electron microscopy, and the cell biological aspects of the organelle biogenesis have seen substantial progress very recently. Likewise, the phenomenon of peroxisome proliferation induced by a number of drugs, e. g. hypolipidemics and plasticizers, constitute an active area of research, where much is still to be learnt. The medical problem of inborn errors of metabolism seems to have gained a new realm for discovery. Here, the advances in knowledge within the last year have been particularly striking. Therefore, the "International Symposium on Peroxisomes in Biology and Medicine," held in Heidelberg from July 14-17,1986, was even more excit- ing than could have initially been anticipated. The present volume consists of papers from that conference. For sake of rapid publication, the camera- ready format, inspite of all its drawbacks (e. g. no proof reading by authors), was chosen. It is our pleasure to acknowledge support and help. The Symposium was supported by Deutsche Forschungsgemeinschaft, Bonn, by the Ministerium ftir Wissenschaft und Kunst Baden-Wtirttemberg, Stuttgart, as well as by a number of generous contributors from industry.
Автор: Michael Schrader Название: Peroxisomes ISBN: 1493969358 ISBN-13(EAN): 9781493969357 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation
Andreas Manner and Markus Islinger
2. Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes
Miriam J. Schцnenberger and Werner J. Kovacs
3. Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays
Tвnia Francisco, Ana F. Dias, Ana G. Pedrosa, Clбudia P. Grou, Tony A. Rodrigues, and Jorge E. Azevedo
4. Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces Cerevisiae
Tobias Hansen, Anna Chan, Thomas Schrцter, Daniel Schwerter, Wolfgang Girzalsky, and Ralf Erdmann
5. Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic Acid
Malu-Clair van de Beek, Inge M.E. Dijkstra, and Stephan Kemp
6. Analysis of Plasmalogen Synthesis in Cultured Cells
Masanori Honsho and Yukio Fujiki
7. Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies
Janet Koster and Hans R. Waterham
8. siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian Cells
Tina A. Schrader and Michael Schrader
9. Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals
Julia Hofhuis, Severin Dieterle, Rosemol George, Fabian Schueren, and Sven Thoms
10. Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3'-diaminobenzidine
H. Dariush Fahimi
11. Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins
Karina Mildner and Dagmar Zeuschner
12. Detection and Immunolabelling of Peroxisomal Proteins
Tina Schrader, Markus Islinger, and Michael Schrader
13. Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago Maydis
Sofia C. Guimaraes, Sreedhar Kilaru, Michael Schrader, and Martin Schuster
14. Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based Probes
Celien Lismont, Paul A. Walton, and Marc Fransen
15. KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress
Marc Fransen and Chantal Brees
16. Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed
Luis F. Godinho, and Michael Schrader
17. In Cellulo Approaches to Study Peroxisomal Protein Import - Yeast Immunofluorescence Microscopy
Tobias Hansen, Wolfgang Girzalsky, and Ralf Erdmann
18. Blue Native Page: Applications to Study Peroxisome Biogenesis
Kanji Okumoto, Shigehiko Tamura, and Yukio Fujiki
19. In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes
Yuqiong Liu, Masanori Honsho, and Yukio Fujiki
20. Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System
Kanji Okumoto, Masanori Honsho, Yuqiong Liu, and Yukio Fujiki
21. The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian Cells
Judith Buentzel, and Sven Thoms
22. Detection of Ubiquitinated Peroxisomal Proteins in Yeast
Natasha Danda and Chris Williams
23. Assessing Pexophagy in Mammalian Cells
Shun-ichi Yamashita and Yukio Fujiki
24. Experimental Systems to Study Yeast Pexophagy
Shun-ichi Yamashita, Masahide O
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