MHC Protocols, Stephen H. Powis; Robert W. Vaughan

Автор: Steven H. Gillespie, Timothy D. McHugh
Название: Antibiotic Resistance Protocols
ISBN: 160327278X ISBN-13(EAN): 9781603272780
Издательство: Springer
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Описание: This book fully updates and builds upon its first edition. Beginning with chapters on epidemiology and population genetics, it continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance.

Автор: Ulrich Reineke, Mike Schutkowski
Название: Epitope Mapping Protocols
ISBN: 1934115177 ISBN-13(EAN): 9781934115176
Издательство: Springer
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Цена: 59290.00 T
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Описание: Given the versatile utility of the determination of epitopes, the need for a thorough collection of experimental protocols is clear. This title offers various applications, including approaches to antibody or B-cell epitope mapping and T-cell epitope mapping and a section on the profiling of antibody signatures in biological fluids.

Автор: Kelly M. Fulton (Editor), Susan M. Twine (Editor)
Название: Immunoproteomics: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627035885 ISBN-13(EAN): 9781627035880
Издательство: Springer
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Цена: 158380.00 T
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Описание: Providing diverse, up-to-date approaches and techniques for studying the immunoproteome, this Methods in Molecular Biology book offers materials lists, step-by-step reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Автор: Michael Steinitz (Editor)
Название: Human Monoclonal Antibodies: Methods and Protocols
ISBN: 1627035850 ISBN-13(EAN): 9781627035859
Издательство: Springer
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Описание: This book details readily reproducible protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies. It also includes articles on related topics of human monoclonal and polyclonal antibodies.

Автор: Gwyn Davies (Editor)
Название: Vaccine Adjuvants: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1607615843 ISBN-13(EAN): 9781607615842
Издательство: Springer
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Описание: The study of vaccine adjuvants has developed into an exciting area of work. In this authoritative and versatile book, expert researchers in the field provide clear and concise guidance on how to go about assessing the activity of adjuvant products.

Автор: Andrea A. Zachary (Editor), Mary S. Leffell
Название: Transplantation Immunology: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627034927 ISBN-13(EAN): 9781627034920
Издательство: Springer
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Описание: PART I: Overview Chapters1. Alloreactivity

Sidonia B.G. Eckle, Jamie Rossjohn, and James McCluskey

2. Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody
Binding and Mechanisms of Injury

Nicole M. Valenzuela and Elaine F. Reed

3. Cell Mediated Rejection

Kathryn J Wood, Masaaki Zaitsu and Ryoichi Goto

4. Transplantation Tolerance

Colin Brinkman, Bryna Burrell, Joseph Scalea, and Jonathan Bromberg

5. Composite Tissue Transplantation

Gerald Brandacher

6. Transplantation of the Sensitized Patient: Histocompatibility Testing

Robert A. Montgomery, Mary S. Leffell, and Andrea A. Zachary

7. Thoracic Organ Transplantation: Laboratory Methods

Jignesh K. Patel and Jon A. Kobashigawa

PART II: Methods Chapters

8. HLA Typing by Sequence Specific Primers

Mike Bunce, Biofortuna and Ben Passey, Biofortuna

9. Human Leukocyte Antigen (HLA) Typing By DNA Sequencing

Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, Carolyn and Katovich Hurley

10. Next-Generation HLA Sequencing using the 454 GS FLX System

Elizabeth A. Trachtenberg and Cherie L. Holcomb

11. Chimerism Testing by Quantitative PCR Using Indel Markers

Ketevan Gendzekhadze, Laima Gaidulis, and David Senitzer

12. Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR)

Raja Rajalingam and Elham Ashouri

13. Complement-dependent Cytotoxicity Crossmatch

Jeremy Ryan Peсa, Donna Fitzpatrick, and Susan L. Saidman

14. Lymphocyte Crossmatching by Flow Cytometry

Robert A. Bray

15. Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies

John D Smith and Marlene L Rose

16. C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens

Ge Chen and Dolly B. Tyan

17. Tetramer Staining for the Detection of HLA-specific B cells

Donna P. Lucas, Mary S. Leffell, and Andrea A. Zachary

18. A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood

Annette M. Jackson, Donna P. Lucas, Jessica L. Badders

19. The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation

Duska Dragun

20. Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA)

Nayan J. Sarma, Venkataswarup Tiriveedhi, and T. Mohanakumar

21. Cylex ImmuKnow Cell Function Assay

Adriana Zeevi and John Lunz

22. Detection of Intracellular Cytokines

Nancy L. Reinsmoen and Chih-Hung Lai

23. Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients

George Vlad and Nicole Suciu-Foca

24. Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA

Tara K. Sigdel, and Minnie. M. Sarwal

25. RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing

Steven R. Head, Tony Mondala, Terri Gelbart, and Phillip Ordoukhanian

Автор: Irving C. Allen (Editor)
Название: Mouse Models of Innate Immunity: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627034803 ISBN-13(EAN): 9781627034807
Издательство: Springer
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Цена: 139750.00 T
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Описание: The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense;

Автор: De Ley
Название: Cytokine Protocols
ISBN: 1617794384 ISBN-13(EAN): 9781617794384
Издательство: Springer
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Цена: 111790.00 T
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Описание: “Recent studies have discovered new known and characterized cytokines, allowing for advancement in miniaturization of micro-analytical methods as well as the extensive development of bio-informatics and nanotechnology.  These advancements have allowed researchers to  reduces sample sizes making for more accurate determinations then previously possible. In Cytokine Protocols: Second Edition, expert researchers in the field detail many of the methods which are now commonly used to study cytokines. These methods and techniques for studying cytokines include historical importance and the importance of researchers using bioassay, quantification, and characterization of cytokine related RNAs,  posttranscriptional modifications of RNA, either naturally or artificially, and observations at the protein level. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and practical, Cytokine Protocols: Second Edition seeks to aid scientists in furthering the crucially important advancement of cytokine research.”

Автор: Ludewig Burkhard, Hoffmann Matthias W.
Название: Adoptive Immunotherapy / Methods and Protocols
ISBN: 1588294064 ISBN-13(EAN): 9781588294067
Издательство: Springer
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Цена: 163040.00 T
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Описание: An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

Автор: Kerry S. Campbell
Название: Natural Killer Cell Protocols: Cellular and Molecular Methods (Methods in Molecular Biology, Vol. 612)
ISBN: 1607613611 ISBN-13(EAN): 9781607613619
Издательство: Springer
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Цена: 204970.00 T
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Описание: Research has revealed that natural killer (NK) cells are sentinels of the innate immune response. This text offers detailed protocols plus the latest methods and techniques designed to study NK cells within specialized tissue sites in both mice and humans.

Автор: Quinn
Название: Neutrophil Methods and Protocols
ISBN: 1627038442 ISBN-13(EAN): 9781627038447
Издательство: Springer
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Цена: 204970.00 T
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Описание: procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression;

Название: Dendritic cell protocols
ISBN: 1607614200 ISBN-13(EAN): 9781607614203
Издательство: Springer
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Цена: 158380.00 T
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Описание: This updated book presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. It aims to become a bench-side handbook for both beginners and experts.