Epitope Mapping Protocols, Ulrich Reineke; Mike Schutkowski (Eds.)
Название: Epitope Mapping: A Practical Approach ISBN: 0199636532 ISBN-13(EAN): 9780199636532 Издательство: Oxford Academ Рейтинг: Цена: 89830 T Наличие на складе: Поставка под заказ. Описание: Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells. The identification of combined B and T cell epitopes can also be achieved using synthetic peptides. There are other methodologies for analysing for cytotxic T cell epitopes such as the purification of antigens presented by MHC class I molecules and expression cloning. Site directed mutagenesis is also a powerful tool in epitope mapping and can be used to evaluate the role of single amino acids in immune complex formation. Protein footprinting makes use of monoclonal antibodies produced by hybridoma technology and relies on the fact that the epitope is protected from cleavage when bound as an antibody-antigen complex. It is only useful for small antigens. Other monoclonal antibody assays such as enzyme linked immunosorbent assay and haemaglutination and slot-blotting may also be used in epitope mapping. Random phage display libraries bring together the genetic and amino acid peptide sequence and can be screened with antibody and the resulting peptide DNA sequenced to confirm the amino acid sequence of a specific eptiope. Investigation of carbohydrates can also be useful to eptitope mapping as deglycosylation can lead to loss of antigenic activity. Epitopes are important to the pharmaceutical industry and wherever appropriate, pharmaceutical applications of the methods described are included. For each method there is a description of the technology, protocols, trouble-shooting, and advice on when to use the method. This book will therefore be invaluable to any researcher involved in epitope mapping.
Автор: Eibl M., Mayr W.R., Thorbecke G.J. Название: Epitope Recognition Since Landsteiner`s Discovery / 100 Years Since the Discovery of Human Blood Groups ISBN: 3540426515 ISBN-13(EAN): 9783540426516 Издательство: Springer Рейтинг: Цена: 72350 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Karl Landsteiner is best known for his discovery of the human blood goups. The revolutionary discoveries of this brilliant scientist in other fields have not received the recognition they deserve. His demonstration that poliomyelitis is transmissable showed the way of modern virology. His studies opening the field for epitope recognition, which he himself considered his main achievement, laid the foundation for research ongoing in our days. This book with its outstanding contributors is but a small tribute to this visionary scientist.
Автор: Westwood Название: Epitope Mapping.2001 ISBN: 0199636524 ISBN-13(EAN): 9780199636525 Издательство: Oxford Academ Рейтинг: Цена: 52660 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: "Epitope Mapping" covers all the major methods for the identification and definition of epitopes. This book is intended for researchers in academia and industry involved with epitope mapping.
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