Proteomic and Metabolomic Approaches to Biomarker Discovery,, Haleem J. Issaq

Автор: Lammerhofer
Название: Metabolomics in Practice: Successful Strategies to Generate and Analyze Metabolic Data
ISBN: 3527330895 ISBN-13(EAN): 9783527330898
Издательство: Wiley
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Цена: 99210.00 T
Наличие на складе: Поставка под заказ.
Описание: Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation.

Автор: Armstrong Donald
Название: Oxidative Stress Biomarkers and Antioxidant Protocols
ISBN: 0896038505 ISBN-13(EAN): 9780896038509
Издательство: Springer
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Цена: 144410.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.

Автор: Pawel Ciborowski
Название: Proteomic Profiling and Analytical Chemistry
ISBN: 0444636889 ISBN-13(EAN): 9780444636881
Издательство: Elsevier Science
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Цена: 116780.00 T
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Описание:

"Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition" helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study.

The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics.
Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measureIncludes practical examples illustrating the importance of problems in quantitation and validation of biomarkersHelps in designing and executing proteomic experiments with sound analytics

Автор: Nedelkov Dobrin, Nelson Randall W.
Название: New and Emerging Proteomic Techniques
ISBN: 1588295192 ISBN-13(EAN): 9781588295194
Издательство: Springer
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Цена: 135090.00 T
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Описание: Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular Biologyв„ў series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Автор: Michael A. Tainsky (Ed.)
Название: Tumor Biomarker Discovery
ISBN: 1603278109 ISBN-13(EAN): 9781603278102
Издательство: Springer
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Цена: 135090.00 T
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Описание: Through a variety of mechanisms, cancer cells provide the biomarker material that can lead to their own detection, which grants clinicians the opportunity for non-invasive body fluid and tissue analysis able to indicate the presence of tumors or the level of tumor burden. This title focuses on use and development of panels of biomarker analytes.

Автор: Dominique Rolin
Название: Metabolomics Coming of Age with its Technological Diversity,67
ISBN: 0123979226 ISBN-13(EAN): 9780123979223
Издательство: Elsevier Science
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Цена: 166190.00 T
Наличие на складе: Поставка под заказ.
Описание: Publishes reviews on a range of topics in plant sciences. This title features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. It features reviews on metabolomics coming of age with its technological diversity.