The development of suitable assays, the integration of appropriate technology, and the effective management of the essential infrastructure are all critical to the success of any high-throughput screening (HTS) endeavor. However, few scientists have the multidisciplinary experience needed to control all aspects of an HTS drug discovery project. A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery integrates the experience of diverse experts who offer fundamental and practical guidance across numerous situations.
The book first discusses assay developments for important target classes such as protein kinases and phosphatases, proteases, nuclear receptors, G protein-coupled receptors, ion channels, and heat shock proteins. It next examines assay developments for cell viability, apoptosis, and infectious diseases. The contributors explore the application of emerging technologies and systems, including image-based high content screening, RNA interference, and primary cells. Finally, they discuss the essential components of the integrated HTS process, such as screening automation, compound library management, the screening of natural products from botanical sources, and screening informatics.
Designed to motivate researchers to bring further advances to the field, this volume provides practical guidance on how to initiate, validate, optimize, and manage a bioassay intended to screen large collections of compounds. Drawing on the knowledge from experts actively involved in assay development and HTS, this is a resource that is both comprehensive and focused.
Автор: Mark Wigglesworth, Dave Murray, Geoff Holdgate, Carolyn J Blackett Название: High Throughput Screening: Methods, Techniques and Applications ISBN: 1536172480 ISBN-13(EAN): 9781536172485 Издательство: Nova Science Рейтинг: Цена: 215410.00 T Наличие на складе: Невозможна поставка. Описание: High Throughput Screening (HTS) is one of several hit identification approaches that are part of a developing and evolving toolbox for the discovery of pharmaceutical start points. HTS remains one of the most successful approaches, and therefore an important foundation of drug discovery. In High Throughput Screening: Methods, Techniques and Applications, leading industrial and academic experts in screening and drug discovery explain key technologies and methods while demonstrating how they can be applied to successful hit identification. Describing both traditional and emerging methods in detail, this book provides an overview of these methods to the reader that will serve both those new to the field and expert scientists alike. High Throughput Screening: Methods, Techniques and Applications provides readers with an outline of key elements in the areas of assay development, detailed descriptions of a range of both biochemical and cell-based screening methodologies and strategies, as well as highlighting important steps in data analysis. By describing the basic principles of methods commonly used in HTS, High Throughput Screening: Methods, Techniques and Applications provides an illuminating introduction to HTS, capturing established good practice within the field, thereby imparting both the industrial and academic researcher with the knowledge required to work effectively in both todays and the hit identification laboratories of the future.
Автор: Zhu Hao Название: High-Throughput Screening Assays in Toxicology ISBN: 1071622129 ISBN-13(EAN): 9781071622124 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models.
Автор: Kisaalita, William S. Название: 3D Cell-Based Biosensors in Drug Discovery Programs ISBN: 1420073494 ISBN-13(EAN): 9781420073492 Издательство: Taylor&Francis Рейтинг: Цена: 209270.00 T Наличие на складе: Нет в наличии.
Автор: Zhu Название: High-Throughput Screening Assays in Toxicology ISBN: 1071622153 ISBN-13(EAN): 9781071622155 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models. This volume also covers the application of artificial intelligence (AI) and data science to curate, manage, and use HTS data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, High Throughput Screening Assays in Modern Toxicology, Second Edition is a valuable resource for scientists pursuing chemical toxicology research. This book will aid scientists and researchers in translating new HTS techniques into standardized chemical toxicology assessment tools that can refine, reduce, and replace animal testing.
Автор: Janzen William P. Название: High Throughput Screening: Methods and Protocols ISBN: 1493981137 ISBN-13(EAN): 9781493981137 Издательство: Springer Рейтинг: Цена: 106010.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Design and Implementation of High Throughput Screening Assays David J. Powell, Robert P. Hertsberg, and Ricardo Macarrόn 2. Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method Thomas V. Riera, Tim J. Wigle, and Robert A. Copeland
3. High Throughput Screening Using Mass Spectrometry within Drug Discovery Mattias Rohman and Jonathan Wingfield 4. Structure-Based Virtual Screening of Commercially Available Compound Libraries Dmitri Kireev 5. AlphaScreen-Based Assays: Ultra-High Throughput Screening for Small Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions Adam Yasgar, Ajit Jadhav, Anton Simeonov, and Nathan P. Coussens 6. Instrument Quality Control Chatura Jayakody and Emily A. Hull-Ryde 7. Application of Fluorescence Polarization in HTS AssaysXinyi Huang and Ann Aulabaugh 8. Time Resolved Fluorescence Assays Chen-Ting Ma and Eduard A. Sergienko 9. Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays Peter Drueckes 10. Screening for Inhibitors of Kinase Autophosphorylation Bianca Heedmann and Martin Klumpp 11. A Fluorescence-Based High Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus Thomas M. Smith, Daryl L. Richie, Jianshi Tao 12. Mycobacterium tuberculosisHigh Throughput Screening E. Lucile White, Nichole A. Tower, and Lynn Rasmussen 13. Identification of State-Dependent Blockers for Voltage Gated Calcium Channels Using a FLIPR Based Assay Alberto di Silvio 14. A Luciferase Reporter Gene System for High Throughput Screening of γ-GlobinGene Activators Wensheng Xie, Robert Silvers, Michael Ouellette, Zining Wu, Quinn Lu, Hu Li, Kathleen Gallagher, Kathy Johnson, and Monica Montoute 15. A High Throughput Flow Cytometry Assay for Identification of Inhibitors of 3', 5'-Cyclic Adenosine Monophosphate Efflux Dominique Perez, Peter C. Simons, Yelena Smagley, Larry A. Sklar, and Alexandre Chigaev 16. High Throughput Cell Toxicity Assays David Murray, Lisa McWilliams, and Mark Wigglesworth 17. BRETn: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells Xiu-Lei Mo and Haian Fu 18. Application of Imaging-Based Assays in Microplate Formats for High Content Screening Adam I. Fogel, Scott E. Martin, and Samuel A. Hasson
Автор: Renaud Vincentelli Название: High-Throughput Protein Production and Purification ISBN: 1493996231 ISBN-13(EAN): 9781493996230 Издательство: Springer Рейтинг: Цена: 204040.00 T Наличие на складе: Поставка под заказ. Описание: This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.
Background.- High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire.- In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire.- Paired VH: VL Analysis of Naпve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors.- Conclusions and Future Perspectives.- Appendices.
Автор: Gordan Lauc Название: High-Throughput Glycomics and Glycoproteomics ISBN: 1493964917 ISBN-13(EAN): 9781493964918 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Поставка под заказ. Описание: This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughout with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and thorough, High-Throughout Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field.
Автор: Anandhakumar Chandran Название: Advancing Development of Synthetic Gene Regulators ISBN: 9811348995 ISBN-13(EAN): 9789811348990 Издательство: Springer Рейтинг: Цена: 93160.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.
Название: Structural Genomics and High Throughput Structural Biology ISBN: 0367392232 ISBN-13(EAN): 9780367392239 Издательство: Taylor&Francis Рейтинг: Цена: 65320.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Researchers in structural genomics continue to search for biochemical and cellular functions of proteins as well as the ways in which proteins assemble into functional pathways and networks using either experimental or computational approaches. Based on the experience of leading international experts, Structural Genomics and High Throughput Structural Biology details state-of-the-art analytical and computational methods used to reveal the three-dimensional structure and function of proteins.
A historical perspective and a detailed guide to the production of protein material for structural determination, a key step in the process, lay the necessary foundation for discussing the most effective structure determination technologies, such as X-ray crystallography and NMR spectroscopy. Encouraging the study of genes and proteins of unknown structure in order to discover new information about folding, specific structural features, or function, Structural Genomics and High Throughput Structural Biology presents the methods used to interpret the sequences of proteins in a structural context, giving insight into their function. It also explains how to extract information from public data repositories and how to account for variability and accuracy in the quality of this data.
The book concludes with a discussion of practical applications of therapeutically driven structural genomics, and presents future directions in the field. Structural Genomics and High Throughput Structural Biology offers a comprehensive guide to the theoretical, technological, and experimental methodologies used to derive structural information from encoded proteins by renowned and world leading scientists in the field.
Автор: Marvin Jens Название: Dissecting Regulatory Interactions of RNA and Protein ISBN: 3319070819 ISBN-13(EAN): 9783319070810 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: The work described in this book is an excellent example of interdisciplinary research in systems biology. It shows how concepts and approaches from the field of physics can be efficiently used to answer biological questions and reports on a novel methodology involving creative computer-based analyses of high-throughput biological data. Many of the findings described in the book, which are the result of collaborations between the author (a theoretical scientist) and experimental biologists and between different laboratories, have been published in high-quality peer-reviewed journals such as Molecular Cell and Nature. However, while those publications address different aspects of post-transcriptional gene regulation, this book provides readers with a complete, coherent and logical view of the research project as a whole. The introduction presents post-transcriptional gene regulation from a distinct angle, highlighting aspects of information theory and evolution and laying the groundwork for the questions addressed in the subsequent chapters, which concern the regulation of the transcriptome as the primary functional carrier of active genetic information.
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