High-Throughput Screening Assays in Toxicology, Zhu Hao
Автор: Zhu Название: High-Throughput Screening Assays in Toxicology ISBN: 1071622153 ISBN-13(EAN): 9781071622155 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models. This volume also covers the application of artificial intelligence (AI) and data science to curate, manage, and use HTS data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, High Throughput Screening Assays in Modern Toxicology, Second Edition is a valuable resource for scientists pursuing chemical toxicology research. This book will aid scientists and researchers in translating new HTS techniques into standardized chemical toxicology assessment tools that can refine, reduce, and replace animal testing.
Автор: Michael Kahn Название: High Throughput Screening for Novel Anti-Inflammatories ISBN: 303489578X ISBN-13(EAN): 9783034895781 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Combinatorial chemistry in conjunction with High Throughput Screening (HTS) is revolutionizing the drug discovery process.
Автор: Janzen William P. Название: High Throughput Screening: Methods and Protocols ISBN: 1493981137 ISBN-13(EAN): 9781493981137 Издательство: Springer Рейтинг: Цена: 106010.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Design and Implementation of High Throughput Screening Assays David J. Powell, Robert P. Hertsberg, and Ricardo Macarrόn 2. Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method Thomas V. Riera, Tim J. Wigle, and Robert A. Copeland
3. High Throughput Screening Using Mass Spectrometry within Drug Discovery Mattias Rohman and Jonathan Wingfield 4. Structure-Based Virtual Screening of Commercially Available Compound Libraries Dmitri Kireev 5. AlphaScreen-Based Assays: Ultra-High Throughput Screening for Small Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions Adam Yasgar, Ajit Jadhav, Anton Simeonov, and Nathan P. Coussens 6. Instrument Quality Control Chatura Jayakody and Emily A. Hull-Ryde 7. Application of Fluorescence Polarization in HTS AssaysXinyi Huang and Ann Aulabaugh 8. Time Resolved Fluorescence Assays Chen-Ting Ma and Eduard A. Sergienko 9. Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays Peter Drueckes 10. Screening for Inhibitors of Kinase Autophosphorylation Bianca Heedmann and Martin Klumpp 11. A Fluorescence-Based High Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus Thomas M. Smith, Daryl L. Richie, Jianshi Tao 12. Mycobacterium tuberculosisHigh Throughput Screening E. Lucile White, Nichole A. Tower, and Lynn Rasmussen 13. Identification of State-Dependent Blockers for Voltage Gated Calcium Channels Using a FLIPR Based Assay Alberto di Silvio 14. A Luciferase Reporter Gene System for High Throughput Screening of γ-GlobinGene Activators Wensheng Xie, Robert Silvers, Michael Ouellette, Zining Wu, Quinn Lu, Hu Li, Kathleen Gallagher, Kathy Johnson, and Monica Montoute 15. A High Throughput Flow Cytometry Assay for Identification of Inhibitors of 3', 5'-Cyclic Adenosine Monophosphate Efflux Dominique Perez, Peter C. Simons, Yelena Smagley, Larry A. Sklar, and Alexandre Chigaev 16. High Throughput Cell Toxicity Assays David Murray, Lisa McWilliams, and Mark Wigglesworth 17. BRETn: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells Xiu-Lei Mo and Haian Fu 18. Application of Imaging-Based Assays in Microplate Formats for High Content Screening Adam I. Fogel, Scott E. Martin, and Samuel A. Hasson
The development of suitable assays, the integration of appropriate technology, and the effective management of the essential infrastructure are all critical to the success of any high-throughput screening (HTS) endeavor. However, few scientists have the multidisciplinary experience needed to control all aspects of an HTS drug discovery project. A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery integrates the experience of diverse experts who offer fundamental and practical guidance across numerous situations.
The book first discusses assay developments for important target classes such as protein kinases and phosphatases, proteases, nuclear receptors, G protein-coupled receptors, ion channels, and heat shock proteins. It next examines assay developments for cell viability, apoptosis, and infectious diseases. The contributors explore the application of emerging technologies and systems, including image-based high content screening, RNA interference, and primary cells. Finally, they discuss the essential components of the integrated HTS process, such as screening automation, compound library management, the screening of natural products from botanical sources, and screening informatics.
Designed to motivate researchers to bring further advances to the field, this volume provides practical guidance on how to initiate, validate, optimize, and manage a bioassay intended to screen large collections of compounds. Drawing on the knowledge from experts actively involved in assay development and HTS, this is a resource that is both comprehensive and focused.
Автор: Mark Wigglesworth, Dave Murray, Geoff Holdgate, Carolyn J Blackett Название: High Throughput Screening: Methods, Techniques and Applications ISBN: 1536172480 ISBN-13(EAN): 9781536172485 Издательство: Nova Science Рейтинг: Цена: 215410.00 T Наличие на складе: Невозможна поставка. Описание: High Throughput Screening (HTS) is one of several hit identification approaches that are part of a developing and evolving toolbox for the discovery of pharmaceutical start points. HTS remains one of the most successful approaches, and therefore an important foundation of drug discovery. In High Throughput Screening: Methods, Techniques and Applications, leading industrial and academic experts in screening and drug discovery explain key technologies and methods while demonstrating how they can be applied to successful hit identification. Describing both traditional and emerging methods in detail, this book provides an overview of these methods to the reader that will serve both those new to the field and expert scientists alike. High Throughput Screening: Methods, Techniques and Applications provides readers with an outline of key elements in the areas of assay development, detailed descriptions of a range of both biochemical and cell-based screening methodologies and strategies, as well as highlighting important steps in data analysis. By describing the basic principles of methods commonly used in HTS, High Throughput Screening: Methods, Techniques and Applications provides an illuminating introduction to HTS, capturing established good practice within the field, thereby imparting both the industrial and academic researcher with the knowledge required to work effectively in both todays and the hit identification laboratories of the future.
Автор: Anandhakumar Chandran Название: Advancing Development of Synthetic Gene Regulators ISBN: 9811065462 ISBN-13(EAN): 9789811065460 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome.
Автор: Naiara Rodr?guez-Ezpeleta; Michael Hackenberg; Ana Название: Bioinformatics for High Throughput Sequencing ISBN: 1489998764 ISBN-13(EAN): 9781489998767 Издательство: Springer Рейтинг: Цена: 148010.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Next generation sequencing is revolutionizing molecular biology. The generation of gigabases of sequence information for each of this huge bandwidth of applications in just a few days makes the development of bioinformatics applications for next generation sequencing data analysis as urgent as challenging.
Автор: Vladimir I. Razinkov Название: High Throughput Formulation Development of Biopharmaceuticals ISBN: 1907568638 ISBN-13(EAN): 9781907568633 Издательство: Elsevier Science Рейтинг: Цена: 162820.00 T Наличие на складе: Поставка под заказ. Описание: High Throughput Formulation Development of Biopharmaceuticals: Practical Guide to Methods and Applications provides the latest developments and information on the science of stable and safe drug product formulations, presenting a comprehensive review and detailed description of modern methodologies in the field of formulation development, a process starting with candidate and pre-formulation screening in its early development phase and then progressing to the refinement of robust formulations during commercialization in the later phases of development. The title covers topics such as experiment design, automation of sample preparation and measurements, high-throughput analytics, stress-inducing methods, statistical analysis of large amounts of formulation study data, emerging technologies, and the presentation of several case studies, along with a concluding summary.
Автор: Anandhakumar Chandran Название: Advancing Development of Synthetic Gene Regulators ISBN: 9811348995 ISBN-13(EAN): 9789811348990 Издательство: Springer Рейтинг: Цена: 93160.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.
Автор: Friedrich Название: Cell Viability Assays ISBN: 1071630512 ISBN-13(EAN): 9781071630518 Издательство: Springer Рейтинг: Цена: 144410.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments.
Автор: Fan Xia; Xiaojin Zhang; Xiaoding Lou; Quan Yuan Название: Biosensors Based on Sandwich Assays ISBN: 9811078343 ISBN-13(EAN): 9789811078347 Издательство: Springer Рейтинг: Цена: 46570.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.
Автор: Zvi Liron; Avraham Bromberg; Morly Fisher Название: Novel Approaches in Biosensors and Rapid Diagnostic Assays ISBN: 1461354528 ISBN-13(EAN): 9781461354529 Издательство: Springer Рейтинг: Цена: 93160.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: In the medical, food, and environmental fields there is a continuous demand for inexpensive and sensitive analytical devices that are reliable, rapid, capable of high-throughput screening, and have low cost per test unit.
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