Автор: Zhu Hao Название: High-Throughput Screening Assays in Toxicology ISBN: 1071622129 ISBN-13(EAN): 9781071622124 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models.
Автор: Fan Xia; Xiaojin Zhang; Xiaoding Lou; Quan Yuan Название: Biosensors Based on Sandwich Assays ISBN: 9811078343 ISBN-13(EAN): 9789811078347 Издательство: Springer Рейтинг: Цена: 46570.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.
Автор: Daniel F. Gilbert; Oliver Friedrich Название: Cell Viability Assays ISBN: 1493983520 ISBN-13(EAN): 9781493983520 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Поставка под заказ.
Автор: Martin J. Stoddart Название: Mammalian Cell Viability ISBN: 1493961535 ISBN-13(EAN): 9781493961535 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This book presents methods for monolayer and suspension cells, methods for determining viability within tissue sections and 3 dimensional culture systems, and methods requiring specialized equipment, all in the popular Methods in Molecular Biology (TM) series format.
Автор: Kreitzer Geri Название: CELL BIOLOGY ASSAYS, ISBN: 0123756928 ISBN-13(EAN): 9780123756923 Издательство: Elsevier Science Рейтинг: Цена: 85430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Protein assay methods are used for protein identification with blood groups, cell surface markers, drugs and toxins. This text features comprehensive protocols essential for researchers studying various areas of biological and medical sciences. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a vareity of technologies and model systems.
Автор: Maximilian Oberleitner Название: Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors ISBN: 3319453831 ISBN-13(EAN): 9783319453835 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Introduction.- Objectives.- Methods & Instrumentation.- MQCM: Multiple Cytomechanic Sensing.- QCM-ECIS: Combined Viscoelastic and Dielectric Sensing of Cells.- QCM-OCS: Optochemical Sensing of Temperature and pO2 in the Cell Surface Junction.- Summary.- Appendix.
Автор: Mohan C Vemuri; Lucas G. Chase; Mahendra S. Rao Название: Mesenchymal Stem Cell Assays and Applications ISBN: 1493957791 ISBN-13(EAN): 9781493957798 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This book shows how to propagate, characterize, and engineer this special cell type. Using the Methods in Molecular Biology (TM) format, each chapter offers an introduction, step-by-step methods, materials list, and tips on troubleshooting and avoiding pitfalls.
Автор: Geri, Kreitzer Название: Cell Biology Assays ISBN: 0123751527 ISBN-13(EAN): 9780123751522 Издательство: Elsevier Science Рейтинг: Цена: 89830.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Presents a comprehensive overview of protocols essential for researchers. This text enables researchers at various career levels a plethora of insight into technologies and model systems in biological problem solving. It features an array of essential methods including endocytic pathwaymembranesces, mitochondira, and vitro motility.
Автор: Mohan C. Vemuri Название: Stem Cell Assays ISBN: 1627039376 ISBN-13(EAN): 9781627039376 Издательство: Springer Рейтинг: Цена: 186330.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Stem cells and regenerative medicine is a fast emerging field with rapid strides of progress and focus on human health. Successful utilization of stem cells in regenerative medicine depends on two important features. One, stem cells can grow and divide indefinitely, and two, stem cells can differentiate into specialized cell types that make up tissues such as pancreas, heart, liver, blood, and others. Utilizing the property of differentiation, stem cells can be engineered to produce cell types to replace wornout cells and to regenerate damaged tissue. The scope for improving healthcare using stem cell therapies is thrilling, but considerable technical challenges and methodological constraints need to be addressed. There is a great need for isolation and propagation of “transplant-ready” or “clinical-grade” stem cells and screening methods, well before they are used in therapies. Recent scientific advances have contributed enormously to the development of new methods pertaining to both embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejection, through polymerase chain reaction approaches following transplantation, and more. The scientific progress that makes stem cell biology so exciting also threatens to suffocate researchers with an avalanche of information. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, the book Stem Cell Assays aims to present scientific advances in stem cell methods for a wider use by novice and expert scientists, through the Methods in Molecular Biology series.
This thesis describes novel substrate embedded physical sensors that can be used to monitor different types of cell-based assays non-invasively and label-free. The sensors described provide integrative information of the cells under study with an adaptable time resolution (ranging from milliseconds to days). This information about the dynamic cell response to chemical, physical or biological stimuli defines a new paradigm in fundamental biomedical research.
The author, Maximilian Oberleitner, describes approaches in which the cells are directly grown on different sensor surfaces (gold-film electrodes, shear wave resonators or dye-doped polymer films). This approach, with the reacting cells in particularly close proximity and contact with the sensor surface, is key to a remarkable sensitivity, opening the way for a variety of new applications. This thesis not only introduces the fundamentals of each approach, but it also describes in great detail the design
principles and elucidates the boundary conditions of the new sensors.
Автор: Martins Название: G Protein-Coupled Receptor Screening Assays ISBN: 1071612239 ISBN-13(EAN): 9781071612231 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses. Whether coming from academia or industry, or being an experienced researcher or a newcomer to the field, the reader will find accessible methods and protocols that cover the latest developments on receptor purification, molecular biology, recombinant engineering, and analytical techniques that enable the real time monitoring of the complex GPCR signaling cascade and identification of potential drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, G Protein-Coupled Receptor Screening Assays: Methods and Protocols, Second Edition aims to provide the tools necessary to contribute to the advancement of GPCR research and discovery and ultimately lead to the availability of innovative and more efficient drugs.
Автор: Lieberman Benjamin A. Название: Steroid Receptor Methods / Protocols and Assays ISBN: 0896037541 ISBN-13(EAN): 9780896037540 Издательство: Springer Рейтинг: Цена: 144410.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: A distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA binding characteristics, hormone binding assays, and the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness.
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