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Bacterial Persistence: Methods and Protocols, Verstraeten Natalie, Michiels Jan
Verstraeten Natalie, Michiels Jan Bacterial Persistence: Methods and Protocols 9781071616208

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Автор: Verstraeten Natalie, Michiels Jan
Название:  Bacterial Persistence: Methods and Protocols
ISBN: 9781071616208
Издательство: Springer
Классификация:
Иммунология

Бактериология (немедицинская)

ISBN-10: 107161620X
Обложка/Формат: Hardcover
Страницы: 323
Вес: 0.68 кг.
Дата издания: 17.09.2021
Язык: English
Размер: 26.16 x 20.32 x 2.03 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: PART I: Introduction

1. Studying [NV1] Bacterial Persistence: Established Methods And Current Advances

Elen Louwagie, Laure Verstraete, Jan Michiels, and Natalie Verstraeten

PART II: Quantification and enrichment of persister cells

2. Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases

Enea Maffei, Cinzia Fino, and Alexander Harms

3. A Robust Method For Generating, Quantifying, and Testing Large Numbers of Escherichia coli persisters

Silke R. Vedelaar, Jakub L. Radzikowski, and Matthias Heinemann

4. Enrichment of Persister Cells through β-lactam-induced Filamentation and Size Separation

Etthel Windels, Bram Van den Bergh, and Jan Michiels

5. Methods for Enrichment of Bacterial Persister Populations For Phenotypic Screens And Genomic Studies

Samantha Adikari, Elizabeth Hong-Geller, and Sofiya Micheva-Viteva

PART III: Single-cell analysis of persister cells

6. Observing Bacterial Persistence at Single-Cell Resolution

Emma Dawson, Emrah Şimşek, and Minsu Kim

7. Phenotypic Characterization of Antibiotic Persisters at the Single-Cell Level: From Data Acquisition to Data Analysis

Nathan Fraikin, Laurence Van Melderen, and Frйdйric Goormaghtigh

8. Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation

Stefany Moreno-Gбmez, Alma Dal Co, Simon van Vliet, and Martin Ackermann

9. Counting Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence

Allison M. Murawski, Katherine Rittenbach, Christina J. DeCoste, Gary Laevsky, and Mark P. Brynildsen

PART IV: Omics approaches in persistence research

10. Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods

Bork A. Berghoff

11. Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa

Pablo Manfredi, Isabella Santi, Enea Maffei, Emmanuelle Lezan, Alexander Schmidt, and Urs Jenal

12. The use of Experimental Evolution to Study The Response of Pseudomonas aeruginosa to Single or Double Antibiotic Treatment

Isabella Santi, Pablo Manfredi, Urs Jenal

PART V: Persister cell resuscitation and eradication

13. Detecting persister awakening determinants

Dorien Wilmaerts, Jan Michiels, and Natalie Verstraeten

14. Monitoring Persister Resuscitation with Flow Cytometry

Sayed Golam Mohiuddin and Mehmet A. Orman

15. Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus persisters

Ashelyn E. Sidders, Lauren C. Radlinski, Sarah E. Rowe, and Brian P. Conlon

PART VI: Cellular and animal model systems for studying persistence

16. In vitro Models for the Study of the Intracellular Activity Of Antibiotics

Frйdйric Peyrusson, Tiep K. Nguyen, Julien M. Buyck, Sandrine Lemaire, Gang W. Wang, Cristina Seral, Paul M. Tulkens, and Van Bambeke

17. Analysis of Salmonella Persister Population Sizes, Dynamics Of Gut Luminal Seeding, And Plasmid Transfer In Mouse Models Of Salmonellosis

Erik Bakkeren, Joshua P. M. Newson, and Wolf-Dietrich Hardt

18. Studying Antibiotic Persistence During Infection

Charlotte Michaux, Severin Ronneau, and Sophie Helaine

Antibiotic Resistance Protocols

Автор: Steven H. Gillespie, Timothy D. McHugh
Название: Antibiotic Resistance Protocols
ISBN: 160327278X ISBN-13(EAN): 9781603272780
Издательство: Springer
Рейтинг:
Цена: 91300.00 T
Наличие на складе: Есть
Описание: This book fully updates and builds upon its first edition. Beginning with chapters on epidemiology and population genetics, it continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance.

Epitope Mapping Protocols

Автор: Ulrich Reineke, Mike Schutkowski
Название: Epitope Mapping Protocols
ISBN: 1934115177 ISBN-13(EAN): 9781934115176
Издательство: Springer
Рейтинг:
Цена: 59290.00 T
Наличие на складе: Есть
Описание: Given the versatile utility of the determination of epitopes, the need for a thorough collection of experimental protocols is clear. This title offers various applications, including approaches to antibody or B-cell epitope mapping and T-cell epitope mapping and a section on the profiling of antibody signatures in biological fluids.

Bacterial Persistence

Автор: Jan Michiels; Maarten Fauvart
Название: Bacterial Persistence
ISBN: 1493928538 ISBN-13(EAN): 9781493928538
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Bacterial Persistence

Bacterial Cell Wall Homeostasis

Автор: Hong
Название: Bacterial Cell Wall Homeostasis
ISBN: 1493936743 ISBN-13(EAN): 9781493936748
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume bringstogether the most widely used and important protocols currently being employedin researching and understanding bacterial cell wall homeostasis. Chapters in BacterialCell Wall Homeostasis cover a variety of subjects, such as: modernmicroscopy techniques and other biophysical methods used to characterize thesubcellular structure of the bacterial cell wall; high-throughput approachesthat can be used to identify all the genes and proteins that participate in thecorrect functioning of an organism’s cell wall; protocols for assayingindividual gene products for specific cell wall functions or identify chemicalswith inhibitory activity against the cell wall; and methods for analyzing the non-proteincomponents of the cell wall and the increasing use of computational approachesfor predicting and modeling cell wall related functions and processes. Writtenin the highly successful Methods in Molecular Biology series format,chapters include introduction to their respective topics, lists of thenecessary material and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Thoroughand cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocolsemphasizes the diversity of the research taking place in bacterial cell wallhomeostasis, and explains how the integration of information from acrossmultiple disciplines is going to be essential if a holistic understanding ofthis important process is to be obtained.

The Bacterial Flagellum

Автор: Tohru Minamino; Keiichi Namba
Название: The Bacterial Flagellum
ISBN: 1493969269 ISBN-13(EAN): 9781493969265
Издательство: Springer
Рейтинг:
Цена: 88500.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation.

Bacterial Protein Secretion Systems

Автор: Laure Journet; Eric Cascales
Название: Bacterial Protein Secretion Systems
ISBN: 1493970313 ISBN-13(EAN): 9781493970315
Издательство: Springer
Рейтинг:
Цена: 186330.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: 1. Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder

Sophie S Abby and Eduardo PC Rocha

2. Protein Sorting Prediction

Henrik Nielsen

3. Cell Fractionation

Melissa Petiti, Laetitia Houot, and Denis Duchй

4. Defining Lipoprotein Localisation by Fluorescence Microscopy

Maria Guillermina Casabona, Mylиne Robert-Genthon, Didier Grunwald, and Ina Attrйe

5. Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate

Nienke Buddelmeijer

6. Defining Membrane Protein Localization by Isopycnic Density Gradients

Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow

7. Components Sub-cellular Localisation: Cell Surface Exposure

Anna Konovalova

8. Probing Inner Membrane Protein Topology by Proteolysis

Maxence S. Vincent and Eric Cascales

9. Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAMTM)

Mikhail Bogdanov

10. Defining Membrane Protein Topology using pho-lac Reporter Fusions

Gouzel Karimova and Daniel Ladant

11. In vivo and in vitro Protein-peptidoglycan Interactions

Gang Li and S. Peter Howard

12. Measure of Peptidoglycan Hydrolase Activity

Yoann G. Santin and Eric Cascales

13. Protein-protein Interaction: Bacterial Two-Hybrid

Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant

14. Protein-protein Interactions: Yeast two-hybrid

Jer-Sheng Lin and Erh-Min Lai

15. Protein-protein Interactions: Cytology Two-hybrid

Krishnamohan Atmakuri

16. Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes

Laureen Logger, Abdelrahim Zoued, and Eric Cascales

17. Protein-protein Interactions: Co-immunoprecipitation

Jer-Sheng Lin and Erh-Min Lai

18. Protein-protein Interaction: Tandem Affinity Purification in Bacteria

Julie P.M. Viala and Emmanuelle Bouveret

19. Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes

Raffaele Ieva

20. Protein-protein Interactions: Pull-down Assays

Arthur Louche, Suzana P. Salcedo, and Sarah Bigot

21. Protein-protein interactions: Surface Plasmon Resonance

Badreddine Douzi

22. Assessing Energy-dependent Protein Conformational Changes in the TonB System

Ray A. Larsen

23. Defining Assembly Pathways by Fluorescence Microscopy

Abdelrahim Zoued and Andreas Diepold

24. Large Complexes: Cloning Strategy, Production, and Purification

Eric Durand and Roland Lloubes

25. Shearing and Enrichment of Extracellular Type IV Pili

Alba Katiria, Gonzalez Rivera, and Katrina T. Forest

26. Blue Native PAGE Analysis of Bacterial Secretion Complexes

Susann Zilkenat, Tobias Dietsche, Julia V. Monjarбs Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner

27. In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography

Gregor L. Weiss, Joгo M. Medeiros, and Martin Pilhofer

28. Structural Analysis of Protein Complexes by Cryo Electron Microscopy

Tiago R. D. Costa, Athanasios Ignatiou, and Elena V. Orlova

29. Bacterial Filamentous Appendages I

Bacterial Transcriptional Control

Автор: Irina Artsimovitch; Thomas J. Santangelo
Название: Bacterial Transcriptional Control
ISBN: 1493923919 ISBN-13(EAN): 9781493923915
Издательство: Springer
Рейтинг:
Цена: 139750.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.

Bacterial Cell Surfaces

Автор: Anne H. Delcour
Название: Bacterial Cell Surfaces
ISBN: 149395993X ISBN-13(EAN): 9781493959938
Издательство: Springer
Рейтинг:
Цена: 135090.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Methods in Molecular Biology (TM) book gathers tools for studying protein structure and function and enzymatic activities, purifying and analyzing of macromolecules and their complexes and investigating regulatory mechanisms and cell biological processes.

Bacterial Molecular Networks

Автор: Jacques van Helden; Ariane Toussaint; Denis Thieff
Название: Bacterial Molecular Networks
ISBN: 149396156X ISBN-13(EAN): 9781493961566
Издательство: Springer
Рейтинг:
Цена: 153720.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Bacterial Molecular Networks provides authoritative descriptions of various experimental and computational methods that enable the characterization and analysis of molecular interaction networks.

Bacterial chromatin

Название: Bacterial chromatin
ISBN: 1493986740 ISBN-13(EAN): 9781493986743
Издательство: Springer
Рейтинг:
Цена: 158380.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume brings together a wide range of methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale. Chapters detail experimental protocols of in vivo and in vitro approaches, approaches to genome structure modeling, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacterial Chromatin: Methods and Protocols aims to be useful as an up-to-date reference work for scholars in the bacterial chromatin field, those entering the field from adjacent research fields, and scientists in the eukaryotic chromatin field.

Bacterial Multidrug Exporters

Автор: Akihito Yamaguchi; Kunihiko Nishino
Название: Bacterial Multidrug Exporters
ISBN: 149398490X ISBN-13(EAN): 9781493984909
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Поставка под заказ.
Описание: This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria. Beginning with protocols for the structural analysis of bacterial multidrug exporters, the book continues with sections on biochemical and bioengineering analysis, computational analysis, biomedical approaches, as well as advanced technologies expected to be applied to multidrug efflux transport studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bacterial Multidrug Exporters: Methods and Protocols serves as an ideal guide to this fast moving field of study.

Bacterial Polysaccharides

Автор: Inka Brockhausen
Название: Bacterial Polysaccharides
ISBN: 1493991531 ISBN-13(EAN): 9781493991532
Издательство: Springer
Рейтинг:
Цена: 186330.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets.
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