Автор: Kerstin Kaufmann; Bernd Mueller-Roeber Название: Plant Gene Regulatory Networks ISBN: 1493971247 ISBN-13(EAN): 9781493971244 Издательство: Springer Рейтинг: Цена: 149060.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Preface... Table of Contents...
Contributing Authors...
1. From Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants
Kerstin Kaufmann and Dijun Chen
Part I Experimental Approaches to Study Plant Gene-Regulatory Networks
2. Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis Bennett Thomson, Emmanuelle Graciet, and Frank Wellmer
3. Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting Daniel Slane and Martin Bayer
4. Characterization of Cell Type-Specific DNA Binding Sites of Plant Transcription Factors using Chromatin Immunoprecipitation On Sun Lau
5. Yeast One and Two Hybrid High-Throughput Screenings using Arrayed Libraries Rocнo Sбnchez-Montesino and Luis Oсate-Sбnchez
6.SELEX-Seq: A Method to Determine DNA-Binding Specificities of Plant Transcription Factors Cezary Smaczniak, Gerco C. Angenent and Kerstin Kaufmann
7. Analysis of a Plant Transcriptional Regulatory Network using Transient Expression Systems Sara Dнaz-Triviсo, Yuchen Long, Ben Scheres, and Ikram Blilou
8.Analysis of In Vivo Chromatin and Protein Interactions of Arabidopsis Transcript Elongation Factors Alexander Pfab, Wojciech Antosz, Philipp Holzinger, Astrid Bruckmann, Joachim Griesenbeck, and Klaus D. Grasser
9.Characterization of Mediator Complex and its Associated Proteins from Rice Subhasis Samanta and Jitendra Kumar Thakur
10. DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing Sergei A. Filichkin and Molly Megraw
11. In Situ Hi-C Library Preparation for Plants to Study their Three-Dimensional Chromatin Interactions on a Genome-Wide Scale Chang Liu
12. Multiplexed Transcriptional Activation or Repression in Plants using CRISPR-dCas9 Based Systems Levi G. Lowder, Joseph W. Paul III, and Yiping Qi
13. Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants Tom Schreiber and Alain Tissier
Part II Computational Approaches to Study Plant Gene-Regulatory Networks
14.Design of Knowledge Bases for Plant Gene Regulatory Networks Eric Mukundi, Fabio Gomez-Cano, Wilberforce Zachary Ouma, and Erich Grotewold
15. AraNet: A Network Biology Server for Arabidopsis thaliana and Other Non-Model Plant Species Tak Lee and Insuk Lee
16. Integration of Genome-Wide TF Binding and Gene Expression Data to Characterize Gene Regulatory Networks in Plant Development Dijun Chen and Kerstin Kaufmann
17. Predicting Transcription Factor-Binding Sites and their Cognate Transcription Factors using Gene Expression Data Chun-Ping Yu and Wen-Hsiung Li
18. Computational Approaches to Study Gene Regulatory Networks Nooshin Omranian and Zoran Nikoloski
19. Boolean Dynamic Modeling Approaches to Study Plant Gene Regulatory Networks: Integration, Validation and Prediction J. Davila-Velderrain, J.C. Martinez-Garcia, and R.E. Alvarez Buylla
20. ODE-Based Modeling of Complex Regulatory Circuits Daniel D. Seaton
21. Inferring Gene Regulatory Networks in the Arabidopsis Root using a Dynamic Bayesian Network Approach Maria Ang
Автор: Bayer Martin Название: Plant Embryogenesis: Methods and Protocols ISBN: 1071603418 ISBN-13(EAN): 9781071603413 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Поставка под заказ. Описание: This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species.
Автор: Popper Zoл a. Название: The Plant Cell Wall: Methods and Protocols ISBN: 1071606190 ISBN-13(EAN): 9781071606193 Издательство: Springer Рейтинг: Цена: 232910.00 T Наличие на складе: Поставка под заказ. Описание: This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology.
Автор: Peter, Isabelle S. Название: Gene Regulatory Networks (Grns),139 ISBN: 0128131802 ISBN-13(EAN): 9780128131800 Издательство: Elsevier Science Рейтинг: Цена: 174050.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Gene Regulatory Networks (GRNs), Volume 139 in the Current Topics in Developmental Biology series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Topics in this release include Mouse hindbrain GRN, Xenopus endoderm GRN - organogenesis, Vertebrate limb GRN, The notochord gene regulatory network in chordate evolution: conservation and divergence from Ciona to vertebrates, Ciona early embryo GRNs, Boolean logic models, Modeling GRN response to morphogen gradient, GRN architecture, Theory of GRN evolution, Evolution of fly segmentation GRNs, GRN evolution in echinoderms, Evolution of network specificity, and more.
Автор: Qi Yiping Название: Plant Genome Editing with Crispr Systems: Methods and Protocols ISBN: 1493989901 ISBN-13(EAN): 9781493989904 Издательство: Springer Рейтинг: Цена: 214280.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Автор: Yung-I Lee; Edward Chee-Tak Yeung Название: Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols ISBN: 1493992805 ISBN-13(EAN): 9781493992805 Издательство: Springer Рейтинг: Цена: 232910.00 T Наличие на складе: Поставка под заказ. Описание: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.
This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation. The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one’s enjoyment.
Автор: Kovalchuk Igor Название: Plant Epigenetics: Methods and Protocols ISBN: 1493979566 ISBN-13(EAN): 9781493979561 Издательство: Springer Рейтинг: Цена: 88500.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis
Wanhui You, Stйphane Pien, and Ueli Grossniklaus
2. Chromatin Conformation Capture-Based Analysis of Nuclear Architecture
Stefan Grob and Ueli Grossniklaus
3. Metaanalysis of Genome-Wide Chromatin Data
Julia Engelhorn and Franziska Turck
4. Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide Probes
Sara Hernбndez-Castellano, Geovanny I. Nic-Can, Clelia De-La-Peсa
5. COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns
Andriy Bilichak and Igor Kovalchuk
6. Analysis of Global Genome Methylation Using the Cytosine-Extension Assay
Andriy Bilichak and Igor Kovalchuk
7. In Situ Analysis Of DNA Methylation in Plants
Palak Kathiria and Igor Kovalchuk
8. Analysis of DNA Hydroxymethylation in Arabidopsis Using Colorimetric Assay
Andrey Golubov and Igor Kovalchuk
9. Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP)
Marнa Бngeles Guevara, Nuria De Marнa, Enrique Sбez-Laguna, Marнa Dolores Vйlez, Marнa Teresa Cervera, and Josй Antonio Cabezas
10. Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized Genomes
Pavlina Sasheva Ueli Grossniklaus
11. Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays
Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe, and Vincent Colot
12. Northern Blotting Techniques for Small RNAs
Todd Blevins
13. Stem-Loop QRT-PCR for the Detection of Plant Micrornas
Erika Varkonyi-Gasic
14. Profiling New Small RNA Sequences
Masayuki Tsuzuki and Yuichiro Watanabe
15. Small RNA Library Preparation and Illumina Sequencing in Plants
Andriy Bilichak, Andrey Golubov, and Igor Kovalchuk
16. Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow
Slava Ilnytskyy and Andriy Bilichak
17. Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression using Virus-Induced Gene Silencing
Andriy Bilichak and Igor Kovalchuk
18. The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks
Andriy Bilichak and Igor Kovalchuk
19. Profiling Transposable Elements and their Epigenetic Effects in Non-Model Species
Christian Parisod
Автор: Dissmeyer Nico, Schnittger Arp Название: Plant Kinases: Methods and Protocols ISBN: 149395721X ISBN-13(EAN): 9781493957217 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Автор: Dandekar Thomas, Naseem Muhammad Название: Auxins and Cytokinins in Plant Biology: Methods and Protocols ISBN: 1493983113 ISBN-13(EAN): 9781493983117 Издательство: Springer Рейтинг: Цена: 88500.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Preface... Table of Contents... Contributing Authors...
Methodological Advances in Auxin and Cytokinin Biology Andrej Hurnэ and Eva Benkovб
Analytical Determination of Auxins and Cytokinins Petre I. Dobrev, Klбra Hoyerovб, and Jan Petrбsek
Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction Jutta Ludwig-Mьller, Susann Auer, Sabine Jьlke, and Sabine Marschollek
Microbial Manipulation of Auxin and Cytokinins in Plants Anwar Hussain, Uhsan Ullah, and Shahida Hasnain
A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling Rocнo Olmo, Ana Clбudia Silva, Fernando E. Dнaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar
Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity Martin Kaltdorf, Dandekar Thomas, and Muhammad Naseem
Interplay between Auxin and Cytokinin and its Impact on Mitogen Activated Protein Kinase (MAPK) Pallavi Singh and Alok Krishna Sinha
Quantification of Cytokinin Levels and Responses in Abiotic Stresses Alfonso Albacete
Assessment of Cytokinin-Induced Immunity through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana Ruth A. Watson and Cris T. Argueso
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies Martin Schдfer and Stefan Meldau
Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface Muhammad Naseem, Shabana Shams, and Thomas Roitsch
Analyzing Cytokinin Responses during Plant-Nematode Interactions Florian M.W. Grundler and Shahid Siddique
Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed
A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks Meik Kunz, Thomas Dandekar, and Muhammad Naseem
Monitoring of Crosstalk between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots Vнctor Carrasco Loba, Marta-Marina Pйrez Alonso, and Stephan Pollmann
High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation Nora Whner, Jцrn Herfert, Wolfgang Drцge-Laser, and Christoph Weiste
Автор: Kleine-Vehn Jьrgen, Sauer Michael Название: Plant Hormones: Methods and Protocols ISBN: 1493982109 ISBN-13(EAN): 9781493982103 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Real Time Analysis of the Apical Hook Development.- Grafting with Arabidopsis thaliana.- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants.- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.- High throughput scoring of Seed Germination.- Histochemical Staining of β-Glucuronidase and its Spatial Quantification.- Imaging TCSn:: GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana.- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins.- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas.- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin.- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells.- In vivo Identification of Plant Protein Complexes using IP-MS/MS.- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons.- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).- Hormone Receptor Glycosylation.- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.- Hormone Profiling in Plant Tissues.- Use of Xenopus laevis Oocytes to Study Auxin Transport.- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.
Автор: Carvalhais Lilia C., Dennis Paul G. Название: The Plant Microbiome: Methods and Protocols ISBN: 1071610392 ISBN-13(EAN): 9781071610398 Издательство: Springer Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.
Автор: Carbonell Alberto Название: Plant Argonaute Proteins: Methods and Protocols ISBN: 1493984047 ISBN-13(EAN): 9781493984046 Издательство: Springer Рейтинг: Цена: 107130.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Plant ARGONAUTEs: Features, Functions and Unknowns
Alberto Carbonell
2. Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5' Cleavage Products of their Target RNAs in Plants
Guodong Ren, Xiaoyan Wang, and Bin Yu
3. In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
Taichiro Iki, Masayuki Ishikawa, and Manabu Yoshikawa
4. In vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
Yukihide Tomari and Hiro-oki Iwakawa
5. Establishment of an In Vivo ARGONAUTE Reporter System in Plants
Kбroly Fбtyol and Jуzsef Burgyбn
6. Immunoprecipitation and High-throughput Sequencing of ARGONAUTE-Bound RNAs from Plants
Alberto Carbonell
7. Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
Ivett Baksa and Gyцrgy Szittya
8. Dissecting the Sub-Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
Cheng-Guo Duan and Jian-Kang Zhu
9. Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana
Cйcile Lecampion, Elodie Lanet, and Christophe Robaglia
10. Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
Marina Martinez-Garciaand Mуnica Pradillo
11. Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas
Tomohito Yamasaki
12. ARGONAUTE genes in Salvia miltiorrhiza: Identification, Characterization and Genetic Transformation
Meizhen Wang, Yuxing Deng, Fenjuan Shao, Miaomiao Liu, Yongqi Pang, Caili Li, and Shanfa Lu
13. Cloning and Characterization of ARGONAUTE Genes in Tomato
Zhiqiang Xian, Fang Yan, and Zhengguo Li
14. Non-Radioactive Detection of Small RNAs Using Digoxygenin-Labeled Probes
Ariel H. Tomassi, Delfina Gagliardi, Damian A. Cambiagno, and Pablo A. Manavella
15. Non-Radioactive Plant Small RNA Detection Using Biotin-Labeled Probes
Jun Hu and Yingguo Zhu
16. Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants
Kai Tang, Cheng-Guo Duan, Huiming Zhang and Jian-Kang Zhu
17. Structural and Functional Characterization of Plant ARGONAUTE MID Domains
Filipp Frank and Bhushan Nagar
18. Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
Andrzej Zielezinski and Wojciech M. Karlowski
19. Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
Joshua T. Trujillo and Rebecca A. Mosher
20. Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES
Ravi K. Singh and Shree P. Pandey
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