Plant Argonaute Proteins: Methods and Protocols, Carbonell Alberto
Автор: Alberto Carbonell Название: Plant Argonaute Proteins ISBN: 1493971646 ISBN-13(EAN): 9781493971640 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
This volume provides the reader with step-by-step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO-small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non-radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.
Автор: Bayer Martin Название: Plant Embryogenesis: Methods and Protocols ISBN: 1071603418 ISBN-13(EAN): 9781071603413 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Поставка под заказ. Описание: This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species.
Автор: Kleine-Vehn Jьrgen, Sauer Michael Название: Plant Hormones: Methods and Protocols ISBN: 1493982109 ISBN-13(EAN): 9781493982103 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Real Time Analysis of the Apical Hook Development.- Grafting with Arabidopsis thaliana.- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants.- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.- High throughput scoring of Seed Germination.- Histochemical Staining of β-Glucuronidase and its Spatial Quantification.- Imaging TCSn:: GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana.- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins.- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas.- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin.- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells.- In vivo Identification of Plant Protein Complexes using IP-MS/MS.- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons.- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).- Hormone Receptor Glycosylation.- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.- Hormone Profiling in Plant Tissues.- Use of Xenopus laevis Oocytes to Study Auxin Transport.- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.
Автор: Spillane Charles, McKeown Peter Название: Plant Epigenetics and Epigenomics: Methods and Protocols ISBN: 1071601784 ISBN-13(EAN): 9781071601785 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Поставка под заказ. Описание: This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.
Автор: Dandekar Thomas, Naseem Muhammad Название: Auxins and Cytokinins in Plant Biology: Methods and Protocols ISBN: 1493983113 ISBN-13(EAN): 9781493983117 Издательство: Springer Рейтинг: Цена: 88500.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Preface... Table of Contents... Contributing Authors...
Methodological Advances in Auxin and Cytokinin Biology Andrej Hurnэ and Eva Benkovб
Analytical Determination of Auxins and Cytokinins Petre I. Dobrev, Klбra Hoyerovб, and Jan Petrбsek
Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction Jutta Ludwig-Mьller, Susann Auer, Sabine Jьlke, and Sabine Marschollek
Microbial Manipulation of Auxin and Cytokinins in Plants Anwar Hussain, Uhsan Ullah, and Shahida Hasnain
A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling Rocнo Olmo, Ana Clбudia Silva, Fernando E. Dнaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar
Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity Martin Kaltdorf, Dandekar Thomas, and Muhammad Naseem
Interplay between Auxin and Cytokinin and its Impact on Mitogen Activated Protein Kinase (MAPK) Pallavi Singh and Alok Krishna Sinha
Quantification of Cytokinin Levels and Responses in Abiotic Stresses Alfonso Albacete
Assessment of Cytokinin-Induced Immunity through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana Ruth A. Watson and Cris T. Argueso
Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies Martin Schдfer and Stefan Meldau
Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface Muhammad Naseem, Shabana Shams, and Thomas Roitsch
Analyzing Cytokinin Responses during Plant-Nematode Interactions Florian M.W. Grundler and Shahid Siddique
Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed
A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks Meik Kunz, Thomas Dandekar, and Muhammad Naseem
Monitoring of Crosstalk between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots Vнctor Carrasco Loba, Marta-Marina Pйrez Alonso, and Stephan Pollmann
High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation Nora Whner, Jцrn Herfert, Wolfgang Drцge-Laser, and Christoph Weiste
Автор: Kovalchuk Igor Название: Plant Epigenetics: Methods and Protocols ISBN: 1493979566 ISBN-13(EAN): 9781493979561 Издательство: Springer Рейтинг: Цена: 88500.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis
Wanhui You, Stйphane Pien, and Ueli Grossniklaus
2. Chromatin Conformation Capture-Based Analysis of Nuclear Architecture
Stefan Grob and Ueli Grossniklaus
3. Metaanalysis of Genome-Wide Chromatin Data
Julia Engelhorn and Franziska Turck
4. Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide Probes
Sara Hernбndez-Castellano, Geovanny I. Nic-Can, Clelia De-La-Peсa
5. COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns
Andriy Bilichak and Igor Kovalchuk
6. Analysis of Global Genome Methylation Using the Cytosine-Extension Assay
Andriy Bilichak and Igor Kovalchuk
7. In Situ Analysis Of DNA Methylation in Plants
Palak Kathiria and Igor Kovalchuk
8. Analysis of DNA Hydroxymethylation in Arabidopsis Using Colorimetric Assay
Andrey Golubov and Igor Kovalchuk
9. Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP)
Marнa Бngeles Guevara, Nuria De Marнa, Enrique Sбez-Laguna, Marнa Dolores Vйlez, Marнa Teresa Cervera, and Josй Antonio Cabezas
10. Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized Genomes
Pavlina Sasheva Ueli Grossniklaus
11. Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays
Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe, and Vincent Colot
12. Northern Blotting Techniques for Small RNAs
Todd Blevins
13. Stem-Loop QRT-PCR for the Detection of Plant Micrornas
Erika Varkonyi-Gasic
14. Profiling New Small RNA Sequences
Masayuki Tsuzuki and Yuichiro Watanabe
15. Small RNA Library Preparation and Illumina Sequencing in Plants
Andriy Bilichak, Andrey Golubov, and Igor Kovalchuk
16. Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow
Slava Ilnytskyy and Andriy Bilichak
17. Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression using Virus-Induced Gene Silencing
Andriy Bilichak and Igor Kovalchuk
18. The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks
Andriy Bilichak and Igor Kovalchuk
19. Profiling Transposable Elements and their Epigenetic Effects in Non-Model Species
Christian Parisod
Автор: Sunkar Ramanjulu Название: Plant Stress Tolerance: Methods and Protocols ISBN: 1493983946 ISBN-13(EAN): 9781493983940 Издательство: Springer Рейтинг: Цена: 107130.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Part I: Review Chapters
1. Epigenetics and RNA Processing: Connections to Drought, Salt and ABA?
Min May Wong, Geeng Loo Chong and Paul Verslues
2. The Fundamental Role Of Reactive Oxygen Species In Stress Response
Michael Liebthal and Karl-Josef Dietz
3. Role of Long Non-coding RNAs on Plant Stress Tolerance
Yuepeng Song and Deqiang Zhang
4. Towards a Resilient, Functional Microbiome: Drought Tolerance-Alleviating Microbes for Sustainable Agriculture
Venkatachalam Lakshmanan, Prasun Ray, and Kelly D. Craven
Part II: Methodology Chapters
5. Mining and Quantifying In Vivo Molecular Interactions in Abiotic Stress Acclimation
Thorsten Seidel and Derya Kirasi
6. Generation of a Stress-inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-responsive Gene Deregulation Mutants
Si-in Yu and Byeong-ha Lee
7. Detection of Differential DNA Methylation under Stress Conditions using Bisulfite-Sequence Analysis
Ibtisam Al Harrasi, Rashid Al-Yahyai and Mahmoud W Yaish
8. ChIP-seq Analysis for Identifying Genome-wide Histone Modifications Associated With Stress-responsive Genes in Plants
Guosheng Li, Guru Jagadeeswaran, Andrew Mort, and Ramanjulu Sunkar
9. Isolation of Polysomal RNA for Analyzing Stress-Responsive Genes Regulated at the Translational Level in Plants
Yong-Fang Li, Ramamurthy Mahalingam, and Ramanjulu Sunkar
10. Global Proteomic Profiling and Identification of Stress-Responsive Proteins using Two-dimensional Gel Electrophoresis
Smita Sahoo, Jay Prakash Awasthi, Ramanjulu Sunkar, and Sanjib Kumar Panda
17. Porous Graphitic Carbon Liquid Chromatography-Mass Spectrometry Analysis of Drought Stress-responsive Raffinose Family Oligosaccharides in Plant Tissues
Tiago F. Jorge, Maria H. Florкncio, and Carla Antуnio
18. Profiling Abscisic Acid Induced Changes in Fatty Acid Composition in Mosses
Suhas Shinde, Shivakumar Devaiah and Aruna Kilaru
19. Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC)
Xiaoqing Gong and Jihong Liu
20. Determination of Polyamines by Dansylation, Benzoylation, and Capillary Electrophoresis
G. Veeranagamallaiah and Chinta Sudhakar
21. Rapid Quantification of Abscisic Acid by GC-MS/MS for studies of Abiotic Stress Response
Paul E. Verslues
22.
Автор: Kaufmann Kerstin, Mueller-Roeber Bernd Название: Plant Gene Regulatory Networks: Methods and Protocols ISBN: 149398392X ISBN-13(EAN): 9781493983926 Издательство: Springer Рейтинг: Цена: 149060.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Preface... Table of Contents...
Contributing Authors...
1. From Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants
Kerstin Kaufmann and Dijun Chen
Part I Experimental Approaches to Study Plant Gene-Regulatory Networks
2. Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis Bennett Thomson, Emmanuelle Graciet, and Frank Wellmer
3. Cell Type-Specific Gene Expression Profiling Using Fluorescence-Activated Nuclear Sorting Daniel Slane and Martin Bayer
4. Characterization of Cell Type-Specific DNA Binding Sites of Plant Transcription Factors using Chromatin Immunoprecipitation On Sun Lau
5. Yeast One and Two Hybrid High-Throughput Screenings using Arrayed Libraries Rocнo Sбnchez-Montesino and Luis Oсate-Sбnchez
6.SELEX-Seq: A Method to Determine DNA-Binding Specificities of Plant Transcription Factors Cezary Smaczniak, Gerco C. Angenent and Kerstin Kaufmann
7. Analysis of a Plant Transcriptional Regulatory Network using Transient Expression Systems Sara Dнaz-Triviсo, Yuchen Long, Ben Scheres, and Ikram Blilou
8.Analysis of In Vivo Chromatin and Protein Interactions of Arabidopsis Transcript Elongation Factors Alexander Pfab, Wojciech Antosz, Philipp Holzinger, Astrid Bruckmann, Joachim Griesenbeck, and Klaus D. Grasser
9.Characterization of Mediator Complex and its Associated Proteins from Rice Subhasis Samanta and Jitendra Kumar Thakur
10. DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing Sergei A. Filichkin and Molly Megraw
11. In Situ Hi-C Library Preparation for Plants to Study their Three-Dimensional Chromatin Interactions on a Genome-Wide Scale Chang Liu
12. Multiplexed Transcriptional Activation or Repression in Plants using CRISPR-dCas9 Based Systems Levi G. Lowder, Joseph W. Paul III, and Yiping Qi
13. Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants Tom Schreiber and Alain Tissier
Part II Computational Approaches to Study Plant Gene-Regulatory Networks
14.Design of Knowledge Bases for Plant Gene Regulatory Networks Eric Mukundi, Fabio Gomez-Cano, Wilberforce Zachary Ouma, and Erich Grotewold
15. AraNet: A Network Biology Server for Arabidopsis thaliana and Other Non-Model Plant Species Tak Lee and Insuk Lee
16. Integration of Genome-Wide TF Binding and Gene Expression Data to Characterize Gene Regulatory Networks in Plant Development Dijun Chen and Kerstin Kaufmann
17. Predicting Transcription Factor-Binding Sites and their Cognate Transcription Factors using Gene Expression Data Chun-Ping Yu and Wen-Hsiung Li
18. Computational Approaches to Study Gene Regulatory Networks Nooshin Omranian and Zoran Nikoloski
19. Boolean Dynamic Modeling Approaches to Study Plant Gene Regulatory Networks: Integration, Validation and Prediction J. Davila-Velderrain, J.C. Martinez-Garcia, and R.E. Alvarez Buylla
20. ODE-Based Modeling of Complex Regulatory Circuits Daniel D. Seaton
21. Inferring Gene Regulatory Networks in the Arabidopsis Root using a Dynamic Bayesian Network Approach Maria Ang
Автор: Yung-I Lee; Edward Chee-Tak Yeung Название: Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols ISBN: 1493992805 ISBN-13(EAN): 9781493992805 Издательство: Springer Рейтинг: Цена: 232910.00 T Наличие на складе: Поставка под заказ. Описание: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.
This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation. The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one’s enjoyment.
Автор: Popper Zoл a. Название: The Plant Cell Wall: Methods and Protocols ISBN: 1071606190 ISBN-13(EAN): 9781071606193 Издательство: Springer Рейтинг: Цена: 232910.00 T Наличие на складе: Поставка под заказ. Описание: This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology.
Автор: Jorrin-Novo Jesus V., Valledor Luis, Castillejo Mari Angeles Название: Plant Proteomics: Methods and Protocols ISBN: 1071605275 ISBN-13(EAN): 9781071605271 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Невозможна поставка. Описание: This third edition details recent advances made in the field of proteomics and the application to plant biology and translational research. In recent years, improvements have been made to protocols on high-throughput proteomics at all workflow stages, from wet to dry labs. Chapters are divided into nine parts, covering methods and protocols such as applications of gel-free, label- or label-free, imaging and targeted approaches to experimental model systems, crops and orphan species, study and analysis of PTMs, protein interactions, specific families of proteins, and finally proteomics in translational research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteomics: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
Автор: Aalen Reidunn Birgitta Название: Plant Receptor Kinases: Methods and Protocols ISBN: 1493983822 ISBN-13(EAN): 9781493983827 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways.
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