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Recombinant Protein Production in Yeast: Methods and Protocols, Bill Roslyn M.


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Автор: Bill Roslyn M.
Название:  Recombinant Protein Production in Yeast: Methods and Protocols
ISBN: 9781493958719
Издательство: Springer
Классификация:


ISBN-10: 1493958712
Обложка/Формат: Paperback
Страницы: 248
Вес: 0.46 кг.
Дата издания: 23.08.2016
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 22 tables, black and white; 6 illustrations, color; 41 illustrations, black and white; xii, 248 p. 47 illus., 6 illus. in color.; 22 tables, black and
Размер: 25.40 x 17.78 x 1.40 cm
Читательская аудитория: General (us: trade)
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Дополнительное описание: Optimising Yeast as a Host for Recombinant Protein Production( review).-Which Yeast Species Shall I Choose? Saccharomyces cerevisiae versus Pichia pastoris (review).-Preparation of Pichia pastoris Expression Plasmids.-Preparation of Saccharomyces cerevisi


Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology

Автор: Otto-Wilhelm Merten; D. Mattanovich; C. Lang; G. L
Название: Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology
ISBN: 9048157560 ISBN-13(EAN): 9789048157563
Издательство: Springer
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Цена: 231990.00 T
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Описание: Selected Articles from the Meeting of the EFB Section on Microbial Physiology, Semmering/A, 5-8 October 2000

Recombinant Protein Production in Yeast

Автор: Brigitte Gasser; Diethard Mattanovich
Название: Recombinant Protein Production in Yeast
ISBN: 1493990233 ISBN-13(EAN): 9781493990238
Издательство: Springer
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Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.

Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology

Автор: Otto-Wilhelm Merten; D. Mattanovich; C. Lang; G. L
Название: Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology
ISBN: 0792371372 ISBN-13(EAN): 9780792371373
Издательство: Springer
Рейтинг:
Цена: 231990.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Selected Articles from the Meeting of the EFB Section on Microbial Physiology, Semmering/A, 5-8 October 2000

Recombinant Glycoprotein Production

Автор: Virg?nia Pican?o-Castro; Kamilla Swiech
Название: Recombinant Glycoprotein Production
ISBN: 1493984497 ISBN-13(EAN): 9781493984497
Издательство: Springer
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Цена: 102480.00 T
Наличие на складе: Поставка под заказ.
Описание: This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols aims to ensure successful results in the further study of this vital field

Recombinant Glycoprotein Production

Автор: Virg?nia Pican?o-Castro; Kamilla Swiech
Название: Recombinant Glycoprotein Production
ISBN: 1493973118 ISBN-13(EAN): 9781493973118
Издательство: Springer
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Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies.

Recombinant Protein Expression in Mammalian Cells

Автор: David L. Hacker
Название: Recombinant Protein Expression in Mammalian Cells
ISBN: 1493993771 ISBN-13(EAN): 9781493993772
Издательство: Springer
Рейтинг:
Цена: 107130.00 T
Наличие на складе: Поставка под заказ.
Описание: This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.

Recombinant Protein Expression in Mammalian Cells

Автор: Hacker
Название: Recombinant Protein Expression in Mammalian Cells
ISBN: 1493987291 ISBN-13(EAN): 9781493987290
Издательство: Springer
Рейтинг:
Цена: 107130.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.

Recombinant and In Vitro RNA Synthesis

Автор: Graeme L. Conn
Название: Recombinant and In Vitro RNA Synthesis
ISBN: 1493958305 ISBN-13(EAN): 9781493958306
Издательство: Springer
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Цена: 111790.00 T
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Описание: Part of Springer`s Methods in Molecular Biology series, this collection of detailed protocols guides the reader from the start of a new RNA project to the final sample, with step-by-step protocols and expert tips on deploying catalytic RNAs in the laboratory.

Recombinant enzymes - from basic science to commercialization

Название: Recombinant enzymes - from basic science to commercialization
ISBN: 3319123963 ISBN-13(EAN): 9783319123967
Издательство: Springer
Рейтинг:
Цена: 130610.00 T
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Описание: Recombinant Enzymes From Basic Science to Commercialization

New Bioprocessing Strategies: Development and Manufacturing of Recombinant Antibodies and Proteins

Автор: Bob Kiss; Uwe Gottschalk; Michael Pohlscheidt
Название: New Bioprocessing Strategies: Development and Manufacturing of Recombinant Antibodies and Proteins
ISBN: 3319971085 ISBN-13(EAN): 9783319971087
Издательство: Springer
Рейтинг:
Цена: 326090.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science.Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification.In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

The Pharmacology of Functional, Biochemical, and Recombinant Receptor Systems

Автор: T. Kenakin; J. A. Angus
Название: The Pharmacology of Functional, Biochemical, and Recombinant Receptor Systems
ISBN: 3642630286 ISBN-13(EAN): 9783642630286
Издательство: Springer
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Цена: 121890.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With contributions by numerous experts

Recombinant Proteins from Plants

Автор: Jacqueline MacDonald; Igor Kolotilin; Rima Menassa
Название: Recombinant Proteins from Plants
ISBN: 1493932888 ISBN-13(EAN): 9781493932887
Издательство: Springer
Рейтинг:
Цена: 88500.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

This volume provides up-to-date scientific achievements from the world's top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.



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