Recombinant DNA Technology, Sardul Singh Sandhu

Автор: Hiroo Imura; Y. Dong; A. Fukamizu; R.H. Goodman; J
Название: Recombinant DNA Technologies in Neuroendocrinology
ISBN: 3642775055 ISBN-13(EAN): 9783642775055
Издательство: Springer
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Описание: 1.1 Mechanism of Action of Glucocorticoid Hormones The current model of glucocorticoid hormone action is summarized in Fig. 1. After synthesis, glucocorticoids are secreted into the blood stream and trans- ported to target cells where they bind with high affinity (K-1O-9M) and d specificity to the intracellular glucocorticoid receptor (GR) protein. The sub- cellular localization of hormone-free GR is still a controversial issue. However, most data support the idea that unliganded GR is in the cytoplasmic compartment or loosely associated with the nucleus (Picard and Yamamoto 1987; Gustafsson et al. 1987 and references therein; LaFond et al. 1988; Gasc et al. 1989). Upon ligand binding, GR is activated into a form capable of interacting with DNA. The mechanism of GR activation probably involves a conformational change and dis- sociation from nonreceptor components, e.g., the 90-kDA heat shock protein (hsp90: Pratt et al. 1988; Bresnick et al. 1989; Denis and Gustafsson 1989). The subcellular location of activated GR has been firmly established to be inside the nucleus. In vivo, the hormone-receptor complex interacts with specific DNA Activation r::: .. qc 3-GC ... &.GC j, BIOLOGICAL EFFECTS " t, Active Protein, Vl\lent.

Автор: Bob Kiss; Uwe Gottschalk; Michael Pohlscheidt
Название: New Bioprocessing Strategies: Development and Manufacturing of Recombinant Antibodies and Proteins
ISBN: 3319971085 ISBN-13(EAN): 9783319971087
Издательство: Springer
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Описание: This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science.Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification.In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Автор: Otto-Wilhelm Merten; D. Mattanovich; C. Lang; G. L
Название: Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology
ISBN: 0792371372 ISBN-13(EAN): 9780792371373
Издательство: Springer
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Описание: Selected Articles from the Meeting of the EFB Section on Microbial Physiology, Semmering/A, 5-8 October 2000

Автор: Balbas Paulina, Lorence Argelia
Название: Recombinant Gene Expression / Reviews and Protocols
ISBN: 1588292622 ISBN-13(EAN): 9781588292629
Издательство: Springer
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Описание: In this second updated edition, the editors have added critical reviews to a collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field the understanding and the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.