RNAi Technologies, Prerna Pandey

Автор: Baohong Zhang; Edmund J. Stellwag
Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells
ISBN: 149395749X ISBN-13(EAN): 9781493957491
Издательство: Springer
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Цена: 130430.00 T
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Описание: Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination. In RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications, expert authors from laboratories across the globe contribute an accessible compendium of up-to-date, proven methods focused on the study of the titular topic. Divided into three sections, the book first gives a brief introduction to RNAi and miRNAs in stem cells, with a focus on the current status of research and future perspectives, then it continues with detailed methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNA-based shRNA, and miRNA expression analysis using qRT-PCR. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications serves as a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and the exciting areas of medicine, including regenerative medicine, aging, cancer, and neurological disorders, that can be advanced through this expanding area of research.

Автор: David O. Azorsa; Shilpi Arora
Название: High-Throughput RNAi Screening
ISBN: 149396335X ISBN-13(EAN): 9781493963355
Издательство: Springer
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Цена: 121110.00 T
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Описание: High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries.--

Автор: Ronald P. van Rij
Название: Antiviral RNAi
ISBN: 1493958259 ISBN-13(EAN): 9781493958252
Издательство: Springer
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Цена: 135090.00 T
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Описание: Here is a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for development and optimization of RNAi-based antiviral drugs. The methods here can also be applied outside the context of a virus infection.

Автор: Zhang
Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells
ISBN: 1607617684 ISBN-13(EAN): 9781607617686
Издательство: Springer
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Цена: 149060.00 T
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Описание: This cutting-edge text will be a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and exciting areas of research such as regenerative medicine and cancer.

Название: RNAi Technology
ISBN: 1138114340 ISBN-13(EAN): 9781138114340
Издательство: Taylor&Francis
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Цена: 71450.00 T
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Описание: RNAi technology are used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. This book reviews the selective and robust effect of RNAi on gene expression which makes it a valuable research tool, both in cell culture and in living organisms because synthetic dsRNA introduced into cells can induce suppression of specific genes of interest. This book will introduce new technology in the study of RNA interference in microorganisms, plants and animals.

Автор: Thangavel; G Sridevi
Название: Environmental sustainability : role of green technologies
ISBN: 8132220552 ISBN-13(EAN): 9788132220558
Издательство: Springer
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Цена: 130610.00 T
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Описание: Covers different categories of green technologies (e.g. biofuels, renewable energy sources, phytoremediation etc.,) in a nutshell -Focuses on next generation technologies which will help to attain the sustainable development -The chapters widely cover for students, faculties and researchers in the scientific arena of Environmentalists, Agriculturalists, Engineers and Policy Makers The World Environment Day 2012 is prepared to embrace green economy. The theme for 2012 encompasses various aspects of human living, ranging from transport to energy to food to sustainable livelihood.

Green technology, an eco-friendly clean technology contributes to sustainable development to conserve the natural resources and environment which will meet the demands of the present and future generations. The proposed book mainly focuses on renewable energy sources, organic farming practices, phyto/bioremediation of contaminants, biofuels, green buildings and green chemistry. All of these eco-friendly technologies will help to reduce the amount of waste and pollution and enhance the nation's economic growth in a sustainable manner.

This book is aimed to provide an integrated approach to sustainable environment and it will be of interest not only to environmentalists but also to agriculturists, soil scientists and bridge the gap between the scientists and policy-makers.

Автор: Barh, Debmalya
Название: Omics Technologies and Bio-engineering
ISBN: 0128046597 ISBN-13(EAN): 9780128046593
Издательство: Elsevier Science
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Цена: 114530.00 T
Наличие на складе: Поставка под заказ.
Описание:

Omics Technologies and Bio-Engineering: Towards Improving Quality of Life is a unique reference that brings together multiple perspectives on omics research, providing in-depth analysis and insights from an international team of authors. The book delivers pivotal information that will inform and improve medical and biological research by helping readers gain more direct access to analytic data, an increased understanding on data evaluation, and a comprehensive picture on how to use omics data in molecular biology, biotechnology and human health care.

Автор: Sara El-Metwally; Osama M. Ouda; Mohamed Helmy
Название: Next Generation Sequencing Technologies and Challenges in Sequence Assembly
ISBN: 149390714X ISBN-13(EAN): 9781493907144
Издательство: Springer
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Цена: 51230.00 T
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Описание: The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.

Автор: G.P. Pfeifer
Название: Technologies for Detection of DNA Damage and Mutations
ISBN: 1489903038 ISBN-13(EAN): 9781489903037
Издательство: Springer
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Цена: 148020.00 T
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Описание: Man-made carcinogens, natural genotoxic agents in the environment, as well as ionizing and ultraviolet radiation can damage DNA and are a constant threat to genome integrity.

Автор: Lobban Christopher S
Название: Writing Undergraduate Lab Reports
ISBN: 1107540240 ISBN-13(EAN): 9781107540248
Издательство: Cambridge Academ
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Цена: 22170.00 T
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Описание: Developing model reports to put advice into practice, this book teaches science undergraduates the structure and content of experimental and observational reports so that they can write a clear and impactful lab report the first time. Annotated published papers are used to demonstrate the transition to writing original-research reports.

Автор: Volker A. Erdmann; Wolfgang Poller; Jan Barciszews
Название: RNA Technologies in Cardiovascular Medicine and Research
ISBN: 3642097464 ISBN-13(EAN): 9783642097461
Издательство: Springer
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Цена: 231990.00 T
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Описание: In this urgently needed book the authors review the field and explore the potential role of RNAi and other RNA technologies in cardiovascular medicine and research. They highlight the impressive recent progress but also the hurdles that must still be overcome.

Автор: Daoliang Li; Chunjiang Zhao
Название: Computer and Computing Technologies in Agriculture III
ISBN: 3642122191 ISBN-13(EAN): 9783642122194
Издательство: Springer
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Цена: 109940.00 T
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Описание: Constitutes the refereed post-conference proceedings of the Third IFIP TC 12 International Conference on Computer and Computing Technologies in Agriculture, CCTA 2009, held in Beijing, China, in October 2009.