Extracellular RNA: Methods and Protocols, Patel Tushar

Автор: Ren-Jang Lin
Название: RNA-Protein Interaction Protocols
ISBN: 1617375551 ISBN-13(EAN): 9781617375552
Издательство: Springer
Рейтинг:
Цена: 153720.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This updated and expanded second edition examines cutting-edge techniques developed recently, along with tried-and-tested methods. Expert contributors explore the isolation and characterization of RNA-protein complexes and related novel techniques.

Автор: Jan Gorodkin; Walter L. Ruzzo
Название: RNA Sequence, Structure, and Function: Computational and Bioinformatic Methods
ISBN: 1493961659 ISBN-13(EAN): 9781493961658
Издательство: Springer
Рейтинг:
Цена: 153720.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The existence of genes for RNA molecules not coding for proteins (ncRNAs) has been recognized since the 1950's, but until recently, aside from the critically important ribosomal and transfer RNA genes, most focus has been on protein coding genes. However, a long series of striking discoveries, from RNA's ability to carry out catalytic function, to discovery of riboswitches, microRNAs and other ribo-regulators performing critical tasks in essentially all living organisms, has created a burgeoning interest in this primordial component of the biosphere. However, the structural characteristics and evolutionary constraints on RNA molecules are very different from those of proteins, necessitating development of a completely new suite of informatic tools to address these challenges. In RNA Sequence, Structure, Function: Computational and Bioinformatic Methods, expert researchers in the field describe a substantial and relevant fraction of these methodologies from both practical and computational/algorithmic perspectives. Focusing on both of these directions addresses both the biologist interested in knowing more about RNA bioinformatics as well as the bioinformaticist interested in more detailed aspects of the algorithms. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results. Thorough and intuitive, RNA Sequence, Structure, Function: Computational and Bioinformatic Methods aids scientists in continuing to study key methods and principles of RNA bioinformatics.

Автор: Jan Gorodkin; Walter L. Ruzzo
Название: RNA Sequence, Structure, and Function: Computational and Bioinformatic Methods
ISBN: 162703708X ISBN-13(EAN): 9781627037082
Издательство: Springer
Рейтинг:
Цена: 186330.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This new publication in the Methods in Molecular Biology series sets out detailed protocols for the targeted bioinformatics procedures dedicated to RNA structure. It features practical and algorithmic approaches as well as expert problem-solving advice.

Автор: Ane Sesma; Tobias von der Haar
Название: Fungal RNA Biology
ISBN: 3319350110 ISBN-13(EAN): 9783319350110
Издательство: Springer
Рейтинг:
Цена: 139310.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: RNA polymerase II-dependent transcription in fungi and its interplay with mRNA decay.- Pre-mRNA splicing and the spliceosome: assembly, catalysis and fidelity.- Fungal pre-mRNA 3' end processing.- mRNA export.- mRNA translation: Fungal variations on a eukaryotic theme.- mRNA localization.- mRNA degradation and decay.- Cytoplasmic mRNA surveillance pathways.- Making Ribosomes: pre-rRNA Transcription and Processing.- Biogenesis and evolution of functional tRNAs.- Small RNA-mediated gene silencing in Neurospora.- The RNAi machinery in Mucorales: the emerging role of endogenous small RNAs.- Regulation of pericentric heterochromatin by ncRNA in Schizosaccharomyces pombe.- Approaches for dissecting RNA-binding protein networks.- Bioinformatic tools for Next-Generation RNA sequencing analysis.

Автор: Hans-Joachim Anders; Adriana Migliorini
Название: Innate DNA and RNA Recognition
ISBN: 1493945831 ISBN-13(EAN): 9781493945832
Издательство: Springer
Рейтинг:
Цена: 97820.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

Part I: Analysis of Viral Nucleic Acid Sensing In-Silico and In-Vitro

1. Detection of RNA Modifications by HPLC Analysis and Competitive ELISA

Gernot Nees, Andreas Kaufmann and Stefan Bauer

2. Enzymatic Synthesis and Purification of a Defined RIG-I Ligand

Marion Goldeck, Martin Schlee, Gunther Hartmann, and Veit Hornung

3. Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis

Filiz Civril and Karl-Peter Hopfner

4. Isolation of RIG-I-associated RNAs from Virus Infected Cells

Andreas Schmidt, Andreas Linder, Nicolas Linder, and Simon Rothenfu er

5. Structure Modeling of Toll-like Receptors

Jing Gong and Tiandi Wei

6. Nucleic Acid Recognition in Dendritic Cells

Alexander Heiseke, Katharina Eisenдcher, and Anne Krug

7. Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems

Andrea Ribeiro and Markus Wцrnle

8. Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro

Simone Romoli, Adriana Migliorini

9. In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes

Saskia Ziegler and Isabelle Bekeredjian-Ding

10. Mapping of Optimal CD8 T Cell Epitopes

Julia Roider, Thomas Vollbrecht and Rika Draenert

11. A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium, and Viral Infection

Viktor H. Koelzer and David Anz

Автор: Frank J. Schmidt
Название: RNA-RNA Interactions
ISBN: 1493941615 ISBN-13(EAN): 9781493941612
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

1. Identifying RNA Recombination Events and Non-covalent RNA-RNA Interactions with the Molecular Colony Technique

Helena V. Chetverina and Alexander B. Chetverin

2. RNA-directed Recombination of RNA in Vitro

Niles Lehman, Nilesh Vaidya, and Jessica A. M. Yeates

3. RNA-RNA Selex

B. Cho

4. Identification of Antisense RNA S0tem-loops that Inhibit RNA-protein Interactions using a Bacterial Reporter System

Kazuo Harada

5. Trans-activation of Large Ribozymes

Matthew B. Martin, Thomas L. Leeper, and Frank J. Schmidt

6. Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies

Arlie J. Rinaldi, Krishna C. Suddala, and Nils G. Walter

7. Single Molecule Studies of RNA-RNA Interactions

Dongmei Yu, Peiwu Qin, and Peter V. Cornish

8. Modification Interference Analysis of the Ribosome

Simpson Joseph

9. Assessing Intermolecular RNA: RNA Interactions Within a Ribonucleoprotein Complex Using Heavy Metal Cleavage Mapping

Keith T. Gagnon and E. Stuart Maxwell

10. Monitoring RNA-RNA Interactions With Electrophoretic Mobility Shift Assays: Analysis Of Trna Binding To The T Box Riboswitch Antiterminator RNA

R. Anupam, S. Zhou, and J.V. Hines

11. Electrophoretic Mobility Shift Assay of RNA-RNA Complexes

Geunu Bak, Kook Han, Kwang-sun Kim, and Younghoon Lee

12. Structural Studies of a Double-stranded RNA from Trypanosome RNA Editing by

Small Angle X-ray Scattering

Angela Criswell and Blaine H.M. Mooers

13. Fusion RNAs in Crystallographic Studies of Double-stranded RNA from

Trypanosome RNA Editing

Blaine H.M. Mooers

Автор: Volker A. Erdmann; Wolfgang Poller; Jan Barciszews
Название: RNA Technologies in Cardiovascular Medicine and Research
ISBN: 3642097464 ISBN-13(EAN): 9783642097461
Издательство: Springer
Рейтинг:
Цена: 231990.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In this urgently needed book the authors review the field and explore the potential role of RNAi and other RNA technologies in cardiovascular medicine and research. They highlight the impressive recent progress but also the hurdles that must still be overcome.

Автор: Volker A. Erdmann; Stefan Jurga; Jan Barciszewski
Название: RNA and DNA Diagnostics
ISBN: 3319361996 ISBN-13(EAN): 9783319361994
Издательство: Springer
Рейтинг:
Цена: 121890.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The aim of molecular diagnostics is preferentially to detect a developing disease before any symptoms appear. Nucleic acid-based medical diagnosis detects specific DNAs or RNAs from the infecting organism or virus and a specific gene or the expression of a gene associated with a disease.

Автор: Mouldy Sioud
Название: RNA Interference
ISBN: 1493948911 ISBN-13(EAN): 9781493948918
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: RNA Interference: Challenges and Therapeutic Opportunities provides readers with recent advances in siRNA design, delivery, targeting and methods to minimize siRNA`s unwanted effects.

Автор: Frank J. Schmidt
Название: RNA-RNA Interactions
ISBN: 1493918958 ISBN-13(EAN): 9781493918959
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA.

Автор: M. Lucrecia Alvarez; Mahtab Nourbakhsh
Название: RNA Mapping
ISBN: 1493953869 ISBN-13(EAN): 9781493953868
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In RNA Mapping- Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study RNA.

Автор: Mouldy Sioud
Название: RNA Therapeutics
ISBN: 1493961519 ISBN-13(EAN): 9781493961511
Издательство: Springer
Рейтинг:
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, the cutting-edge protocol chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes. Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics.