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Innate DNA and RNA Recognition, Hans-Joachim Anders; Adriana Migliorini


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Автор: Hans-Joachim Anders; Adriana Migliorini
Название:  Innate DNA and RNA Recognition
ISBN: 9781493945832
Издательство: Springer
Классификация:
ISBN-10: 1493945831
Обложка/Формат: Paperback
Страницы: 195
Вес: 0.30 кг.
Дата издания: 23.08.2016
Серия: Methods in Molecular Biology
Язык: English
Размер: 234 x 156 x 11
Основная тема: Biomedicine
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание:

Part I: Analysis of Viral Nucleic Acid Sensing In-Silico and In-Vitro

1. Detection of RNA Modifications by HPLC Analysis and Competitive ELISA

Gernot Nees, Andreas Kaufmann and Stefan Bauer

2. Enzymatic Synthesis and Purification of a Defined RIG-I Ligand

Marion Goldeck, Martin Schlee, Gunther Hartmann, and Veit Hornung

3. Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis

Filiz Civril and Karl-Peter Hopfner

4. Isolation of RIG-I-associated RNAs from Virus Infected Cells

Andreas Schmidt, Andreas Linder, Nicolas Linder, and Simon Rothenfu er

5. Structure Modeling of Toll-like Receptors

Jing Gong and Tiandi Wei

6. Nucleic Acid Recognition in Dendritic Cells

Alexander Heiseke, Katharina Eisenдcher, and Anne Krug

7. Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems

Andrea Ribeiro and Markus Wцrnle

8. Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro

Simone Romoli, Adriana Migliorini

9. In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes

Saskia Ziegler and Isabelle Bekeredjian-Ding

10. Mapping of Optimal CD8 T Cell Epitopes

Julia Roider, Thomas Vollbrecht and Rika Draenert

11. A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium, and Viral Infection

Viktor H. Koelzer and David Anz

12. MicroRNA Methodology: Advances in miRNA-technologies

Theresa Kaeuferle, Sabine Bartel, Stefan Dehmel, and Susanne Krauss-Etschmann

Part II: Analysis of Nucleic Acid Sensing In-Vivo

13. Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR)

Maciej Lech and Hans-Joachim Anders

14. Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus

Amanda A. Watkins, Ramon G. B. Bonegio and Ian R. Rifkin

15. Induction and Analysis of Nephrotoxic Serum Nephritis in Mice

John M. Hoppe and Volker Vielhauer

16. Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice

Moritz Rapp, David Anz, and Max Schnurr

17. Bifunctional siRNAs for Tumor Therapy

Fanny Matheis and Robert Besch



Innate Immunity

Автор: Jonathan Ewbank; Eric Vivier
Название: Innate Immunity
ISBN: 1617377546 ISBN-13(EAN): 9781617377549
Издательство: Springer
Рейтинг:
Цена: 135090.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume contains chapters dealing with the isolation and functional characterization of cells involved in innate immunity in mouse and man, including mast cells and eosinophils, and with several chapters focusing on natural killer cells.

DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases

Автор: Volker A. Erdmann; Jan Barciszewski
Название: DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases
ISBN: 3642443524 ISBN-13(EAN): 9783642443527
Издательство: Springer
Рейтинг:
Цена: 156720.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book covers the design and synthesis of DNA and RNA nanostructures with the aim of using them for drug deliveries, for genetic immunization, for metabolite and nucleic acid detection, gene regulation, and siRNA delivery for cancer treatment.

RNA and DNA Diagnostics

Автор: Volker A. Erdmann; Stefan Jurga; Jan Barciszewski
Название: RNA and DNA Diagnostics
ISBN: 3319361996 ISBN-13(EAN): 9783319361994
Издательство: Springer
Рейтинг:
Цена: 121890.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The aim of molecular diagnostics is preferentially to detect a developing disease before any symptoms appear. Nucleic acid-based medical diagnosis detects specific DNAs or RNAs from the infecting organism or virus and a specific gene or the expression of a gene associated with a disease.

DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases

Автор: Volker A. Erdmann; Jan Barciszewski
Название: DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases
ISBN: 3642368522 ISBN-13(EAN): 9783642368523
Издательство: Springer
Рейтинг:
Цена: 174150.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book covers the design and synthesis of DNA and RNA nanostructures with the aim of using them for drug deliveries, for genetic immunization, for metabolite and nucleic acid detection, gene regulation, and siRNA delivery for cancer treatment.

RNA and DNA Diagnostics

Автор: Volker A. Erdmann; Stefan Jurga; Jan Barciszewski
Название: RNA and DNA Diagnostics
ISBN: 3319173049 ISBN-13(EAN): 9783319173047
Издательство: Springer
Рейтинг:
Цена: 139750.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The aim of molecular diagnostics is preferentially to detect a developing disease before any symptoms appear. There has been a significant increase, fueled by technologies from the human genome project, in the availability of nucleic acid sequence information for all living organisms including bacteria and viruses. When combined with a different type of instrumentation applied, the resulting diagnostics is specific and sensitive. Nucleic acid-based medical diagnosis detects specific DNAs or RNAs from the infecting organism or virus and a specific gene or the expression of a gene associated with a disease. Nucleic acid approaches also stimulate a basic science by opening lines of inquiry that will lead to greater understanding of the molecules at the center of life. One can follow Richard Feynman’s famous statement “What I cannot create, I do not understand.”

Bacterial Regulatory RNA

Автор: Kenneth C. Keiler
Название: Bacterial Regulatory RNA
ISBN: 1493959417 ISBN-13(EAN): 9781493959419
Издательство: Springer
Рейтинг:
Цена: 111790.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Methods in Molecular Biology (TM) volume covers methods and techniques to identify regulatory RNAs, characterizing the function and expression of regulatory RNAs in bacterial cells, RNA structure prediction and interaction between regulatory RNAs and proteins.

RNA-RNA Interactions

Автор: Frank J. Schmidt
Название: RNA-RNA Interactions
ISBN: 1493918958 ISBN-13(EAN): 9781493918959
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA.


RNA Nanostructures

Автор: Eckart Bindewald; Bruce A. Shapiro
Название: RNA Nanostructures
ISBN: 1493971379 ISBN-13(EAN): 9781493971374
Издательство: Springer
Рейтинг:
Цена: 139750.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures.

RNA and Cancer

Автор: Jane Y. Wu
Название: RNA and Cancer
ISBN: 3642444857 ISBN-13(EAN): 9783642444852
Издательство: Springer
Рейтинг:
Цена: 158380.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Accumulating evidence supports the role of defects in post-transcriptional gene regulation in the development of cancer. RNA and Cancer examines the recent advances in our understanding of post-transcriptional gene regulation, especially RNA processing and its role in cancer development and treatment. A particular focus is mRNA splicing, but other topics such as microRNAs, mRNA stability, the perinucleolar compartment, and oligonucleotide therapeutics are also covered in detail. All chapters have been written by internationally renowned experts. The book is intended for all with an interest in gene regulation and cancer biology, and especially for those not directly working on RNA biology, including clinicians and medical students. It is hoped that it will stimulate further innovative research collaborations between RNA biologists and cancer researchers to the benefit of patients.

RNA Mapping

Автор: M. Lucrecia Alvarez; Mahtab Nourbakhsh
Название: RNA Mapping
ISBN: 1493910612 ISBN-13(EAN): 9781493910618
Издательство: Springer
Рейтинг:
Цена: 149060.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In RNA Mapping- Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study RNA.

Recombinant and In Vitro RNA Synthesis

Автор: Graeme L. Conn
Название: Recombinant and In Vitro RNA Synthesis
ISBN: 1493958305 ISBN-13(EAN): 9781493958306
Издательство: Springer
Рейтинг:
Цена: 111790.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Part of Springer`s Methods in Molecular Biology series, this collection of detailed protocols guides the reader from the start of a new RNA project to the final sample, with step-by-step protocols and expert tips on deploying catalytic RNAs in the laboratory.

RNA-RNA Interactions

Автор: Frank J. Schmidt
Название: RNA-RNA Interactions
ISBN: 1493941615 ISBN-13(EAN): 9781493941612
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

1. Identifying RNA Recombination Events and Non-covalent RNA-RNA Interactions with the Molecular Colony Technique

Helena V. Chetverina and Alexander B. Chetverin

2. RNA-directed Recombination of RNA in Vitro

Niles Lehman, Nilesh Vaidya, and Jessica A. M. Yeates

3. RNA-RNA Selex

B. Cho

4. Identification of Antisense RNA S0tem-loops that Inhibit RNA-protein Interactions using a Bacterial Reporter System

Kazuo Harada

5. Trans-activation of Large Ribozymes

Matthew B. Martin, Thomas L. Leeper, and Frank J. Schmidt

6. Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies

Arlie J. Rinaldi, Krishna C. Suddala, and Nils G. Walter

7. Single Molecule Studies of RNA-RNA Interactions

Dongmei Yu, Peiwu Qin, and Peter V. Cornish

8. Modification Interference Analysis of the Ribosome

Simpson Joseph

9. Assessing Intermolecular RNA: RNA Interactions Within a Ribonucleoprotein Complex Using Heavy Metal Cleavage Mapping

Keith T. Gagnon and E. Stuart Maxwell

10. Monitoring RNA-RNA Interactions With Electrophoretic Mobility Shift Assays: Analysis Of Trna Binding To The T Box Riboswitch Antiterminator RNA

R. Anupam, S. Zhou, and J.V. Hines

11. Electrophoretic Mobility Shift Assay of RNA-RNA Complexes

Geunu Bak, Kook Han, Kwang-sun Kim, and Younghoon Lee

12. Structural Studies of a Double-stranded RNA from Trypanosome RNA Editing by

Small Angle X-ray Scattering

Angela Criswell and Blaine H.M. Mooers

13. Fusion RNAs in Crystallographic Studies of Double-stranded RNA from

Trypanosome RNA Editing

Blaine H.M. Mooers



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