Автор: Dennison C. Название: A Guide to Protein Isolation ISBN: 1402012241 ISBN-13(EAN): 9781402012242 Издательство: Springer Рейтинг: Цена: 65210.00 T Наличие на складе: Есть Описание: Traffic exists on a different "level" - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile "blobs", with no consideration of how they become mobile.
Автор: Bonner, Philip L.r. Название: Protein purification ISBN: 0415385113 ISBN-13(EAN): 9780415385114 Издательство: Taylor&Francis Рейтинг: Цена: 41850.00 T Наличие на складе: Нет в наличии. Описание: A guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. It also includes an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications.
Автор: Walls Название: Protein Chromatography ISBN: 1607619121 ISBN-13(EAN): 9781607619123 Издательство: Springer Рейтинг: Цена: 153720.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
Автор: Cohen, Sharona Название: Single molecule fluorescence spectroscopy of the folding of a repeat protein ISBN: 3319095579 ISBN-13(EAN): 9783319095578 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Inthis thesis single-molecule fluorescence resonance energy transfer(FRET)spectroscopy was used to study the folding of a protein that belongsto the large and important family of repeat proteins.
Автор: Nalini Chandar Название: Lippincott`s Illustrated Reviews: Cell and Molecular Biology (International Edition) ISBN: 1609133099 ISBN-13(EAN): 9781609133092 Издательство: Lippincott Williams & Wilkins Рейтинг: Цена: 34890.00 T Наличие на складе: Невозможна поставка. Описание: This new title to the best-selling Lippincott's Illustrated Reviews series will present essential coverage of cell and molecular biology focusing on topics related to human health and disease. LIR Cell and Molecular Biology will include the popular featur
Автор: Horwich Arthur L Et Al Название: Structure And Action Of Molecular Chaperones: Machines That Assist Protein Folding In The Cell ISBN: 981474932X ISBN-13(EAN): 9789814749329 Издательство: World Scientific Publishing Рейтинг: Цена: 132000.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This unique volume reviews the beautiful architectures and varying mechanical actions of the set of specialized cellular proteins called molecular chaperones, which provide essential kinetic assistance to processes of protein folding and unfolding in the cell.
Автор: Wang Название: Study of the Peptide-Peptide and Peptide-Protein Interactions and Their Applications in Cell Imaging and Nanoparticle Surface Modification ISBN: 3662533979 ISBN-13(EAN): 9783662533970 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This thesis focuses on the study of interactions between protein and peptides and their potential applications in cell imaging and nanoparticle surface modification. Drawing inspiration from naturally occurring coiled-coil binding pairs, it proposes a novel covalent peptide tag and probe system, based on the concept of “affinity guided covalent conjugation.” This newly established methodology provides complementary resolution to protein labeling, imaging and trafficking. By systematically investigating the coordination interaction between protein and quantum dots using various engineered protein ligands, this thesis proposes a general rule for protein self-assembly on the surface of quantum dots and reports a revolutionized nanobelt protein in accordance with this rule. It is an extraordinary example of interdisciplinary research, providing answers to real-life biological problems from a chemistry perspective.
Автор: James R. Swartz Название: Cell-Free Protein Expression ISBN: 3642639399 ISBN-13(EAN): 9783642639395 Издательство: Springer Рейтинг: Цена: 95770.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified.
Автор: Zvi Cohen Название: Single Cell Oils: Microbial and Algal Oils, Second Ed ISBN: 1893997731 ISBN-13(EAN): 9781893997738 Издательство: Elsevier Science Рейтинг: Цена: 116780.00 T Наличие на складе: Невозможна поставка. Описание: Featuring recognized academic and industrial experts in this cutting-edge field, this book reviews single cell oils (SCO) currently in the market. The text mainly focuses on the production of the long chain polyunsaturated fatty acids, Arachidonic acid, and Docosahexaenoinc acid. All chapters provide up to date references for navigating the vast amount of historic data available in the field. The authors provide real world examples of the commercial development and applications of various SCO in a variety of fields, from food ingredients and disease treatment to aquaculture and fish farming. It covers the essential information in this fast moving field giving details of the production of all the major SCOs, their extraction, purification, applications and safety evaluations. In addition, this new edition includes major coverage of the potential of SCOs for biofuels that may be of key significance in the coming years.
Автор: Bert, van Duijn; Anneke Wiltink Название: Signal Transduction — Single Cell Techniques ISBN: 3642489761 ISBN-13(EAN): 9783642489761 Издательство: Springer Рейтинг: Цена: 113190.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy.
Автор: Xiangdong Wang Название: Single Cell Sequencing and Systems Immunology ISBN: 9401797528 ISBN-13(EAN): 9789401797528 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.
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