Haemostasis: Methods and Protocols, Monagle, Paul (Ed.)

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 13330.00 T
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Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Kwok Pui-Yan
Название: Single Nucleotide Polymorphisms / Methods and Protocols
ISBN: 0896039684 ISBN-13(EAN): 9780896039681
Издательство: Springer
Цена: 38730.00 T
Наличие на складе: Есть
Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Автор: Ludewig Burkhard, Hoffmann Matthias W.
Название: Adoptive Immunotherapy / Methods and Protocols
ISBN: 1588294064 ISBN-13(EAN): 9781588294067
Издательство: Springer
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Цена: 163040.00 T
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Описание: An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

Автор: Lo Benny K. C.
Название: Antibody Engineering / Methods and Protocols
ISBN: 1588290921 ISBN-13(EAN): 9781588290922
Издательство: Springer
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Цена: 163040.00 T
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Описание: A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.

Автор: Proetzel
Название: Antibody Methods and Protocols
ISBN: 1617799300 ISBN-13(EAN): 9781617799303
Издательство: Springer
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Цена: 139750.00 T
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Описание: The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.  Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments.  The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.

Автор: Carr Daniel J. J.
Название: Interferon Methods and Protocols
ISBN: 1588294188 ISBN-13(EAN): 9781588294180
Издательство: Springer
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Цена: 111790.00 T
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Описание: A compendium of methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. This book offers researchers tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of various biological events influenced by IFNs.

Автор: Amini, Shohreh (Editor), White, Martyn K (Editor)
Название: Neuronal Cell Culture: Methods and Protocols (2013) ( Methods in Molecular Biology #1078 )
ISBN: 1627036393 ISBN-13(EAN): 9781627036399
Издательство: Springer
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Цена: 139750.00 T
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Описание: This book details the latest aspects of the culture of neural cells and explains the practical and theoretical considerations of the techniques involved. It features step-by-step, readily reproducible laboratory protocols and expert implementation advice.

Автор: Watkins
Название: Current Protocols Select: Methods and Applications in Microscopy and Imaging
ISBN: 1118044312 ISBN-13(EAN): 9781118044315
Издательство: Wiley
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Цена: 129890.00 T
Наличие на складе: Невозможна поставка.
Описание: The advanced light microscope is a highly sophisticated robotic tool which, when coupled with appropriate detectors and image processing, allows scientists to investigate the inner workings of biological systems from the single molecule, through cells, to

Автор: Andras Perl (Editor)
Название: Autoimmunity: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1607617196 ISBN-13(EAN): 9781607617198
Издательство: Springer
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Цена: 135090.00 T
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Описание: Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.

Автор: Irving C. Allen (Editor)
Название: Mouse Models of Innate Immunity: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627034803 ISBN-13(EAN): 9781627034807
Издательство: Springer
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Цена: 139750.00 T
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Описание: The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense;

Автор: Susanne M. Bailer (Editor), Diana Lieber (Editor)
Название: Virus-Host Interactions: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627036008 ISBN-13(EAN): 9781627036009
Издательство: Springer
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Цена: 158380.00 T
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Описание: This volume covers various aspects of virological research, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level. It features step-by-step, readily reproducible protocols and key implementation advice.

Автор: Michael Steinitz (Editor)
Название: Human Monoclonal Antibodies: Methods and Protocols
ISBN: 1627035850 ISBN-13(EAN): 9781627035859
Издательство: Springer
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Цена: 158380.00 T
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Описание: This book details readily reproducible protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies. It also includes articles on related topics of human monoclonal and polyclonal antibodies.