Gene Cloning and Analysis, Schaefer, B.C.

Автор: Patil Nayana, Sivaram Aruna
Название: A Complete Guide to Gene Cloning: From Basic to Advanced
ISBN: 3030968537 ISBN-13(EAN): 9783030968533
Издательство: Springer
Рейтинг:
Цена: 79190.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.

Автор: Sambrook, Joseph Russell, David
Название: Molecular cloning
ISBN: 0879695773 ISBN-13(EAN): 9780879695774
Издательство: NBN International
Рейтинг:
Цена: 252030.00 T
Наличие на складе: Нет в наличии.
Описание: In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.

Автор: Seok-Yong Choi; Hyunju Ro; Hankuil Yi
Название: DNA Cloning: A Hands-on Approach
ISBN: 9402416609 ISBN-13(EAN): 9789402416602
Издательство: Springer
Рейтинг:
Цена: 139750.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

Автор: Schaefer, B.C.
Название: Gene Cloning and Analysis
ISBN: 1898486069 ISBN-13(EAN): 9781898486060
Издательство: Taylor&Francis
Рейтинг:
Цена: 20410.00 T
Наличие на складе: Нет в наличии.

Автор: Green, Michael R. Sambrook, Joseph
Название: Molecular cloning : a laboratory manual
ISBN: 1936113422 ISBN-13(EAN): 9781936113422
Издательство: INGRAM PUBLISHER SERVICES UK
Рейтинг:
Цена: 335650.00 T
Наличие на складе: Невозможна поставка.
Описание: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standardthe one indispensable molecular biology laboratory manual and reference source.

Автор: Hardin
Название: Cloning Gene Expression
ISBN: 0195132947 ISBN-13(EAN): 9780195132946
Издательство: Oxford Academ
Рейтинг:
Цена: 73920.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The central purpose of this combination lecture/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyzse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Electrophoresis, southern and western blotting and cominatorial techniques are empsized. Radioisotopes, fluorophores and solvent effects on protein structure are discussed. (No radioisotopes are required.) The experiments included have been selected because of their high success rate and because they emphasize a project-oriented approach. Sufficient detail and background are provided such that the intended audience includes advanced undergraduate students, beginning graduates, and practising professionals engaged in a broad range of pursuits. Each experiment is accompanied by detailed process rationale.

Автор: Patil Nayana, Sivaram Aruna
Название: A Complete Guide to Gene Cloning: From Basic to Advanced
ISBN: 3030968502 ISBN-13(EAN): 9783030968502
Издательство: Springer
Рейтинг:
Цена: 111790.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.

Автор: Elser, Amin
Название: Gene editing, epigenetic, cloning and therapy
ISBN: 1524621994 ISBN-13(EAN): 9781524621995
Издательство: Неизвестно
Рейтинг:
Цена: 23440.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Explores the many types and trends of ads issued by both domestic car manufacturers and importers in three main sections: "Family Marques", "Luxury and Sporting Marques" and "Imported Marques". Twelve appendices provide information on various topics ranging from the value of the pound in 1958-70 and imports of cars from selected countries, to car manufacturers` and importers` advertising agencies.

Автор: Faraday Patrick
Название: Gene Cloning
ISBN: 1682865150 ISBN-13(EAN): 9781682865156
Издательство: Неизвестно
Цена: 161990.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.

Автор: Shiv Sanjeevi, Prerna Pandey
Название: Cloning in plants: new developments
ISBN: 1774071762 ISBN-13(EAN): 9781774071762
Издательство: Mare Nostrum (Eurospan)
Рейтинг:
Цена: 164470.00 T
Наличие на складе: Нет в наличии.
Описание: Examines the process of cloning and plant transformation. The book covers the tools required for cloning. The basic requirements of cloning, and cloning and transformation of medicinal plants is also described.

Автор: Joel F. Habener
Название: Molecular Cloning of Hormone Genes
ISBN: 0896030911 ISBN-13(EAN): 9780896030916
Издательство: Springer
Рейтинг:
Цена: 231990.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In addition to provid- ing the coding sequences of recognized hormonal and regulatory peptides, gene sequencing has uncovered new bioactive peptides encoded in the precursor pro hormones that are then liberated along with the hormonal peptides during cellular cleavages of the precursors.

Автор: Valdis Berzins
Название: Basic Cloning Procedures
ISBN: 364248977X ISBN-13(EAN): 9783642489778
Издательство: Springer
Рейтинг:
Цена: 46570.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.