Автор: Brigitte Gasser; Diethard Mattanovich Название: Recombinant Protein Production in Yeast ISBN: 1493990233 ISBN-13(EAN): 9781493990238 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.
Автор: Moulton Название: Fed-Batch Fementation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli ISBN: 1907568921 ISBN-13(EAN): 9781907568923 Издательство: Elsevier Science Рейтинг: Цена: 159450.00 T Наличие на складе: Нет в наличии. Описание: Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.
Автор: Otto-Wilhelm Merten; D. Mattanovich; C. Lang; G. L Название: Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology ISBN: 9048157560 ISBN-13(EAN): 9789048157563 Издательство: Springer Рейтинг: Цена: 231990.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Selected Articles from the Meeting of the EFB Section on Microbial Physiology, Semmering/A, 5-8 October 2000
Recombinant Protein Expression, Part A, Volume 659 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.
Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr(R) 250, and Expression of recombinant antibodies in Leishmania tarentolae.
Название: Recombinant Protein Expression: Eukaryotic Hosts, 660 ISBN: 0323907377 ISBN-13(EAN): 9780323907378 Издательство: Elsevier Science Рейтинг: Цена: 175170.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.
Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr(R) 250, and Expression of recombinant antibodies in Leishmania tarentolae.
Автор: Watson Название: Recombinant dna: short course ISBN: 0716728664 ISBN-13(EAN): 9780716728665 Издательство: Неизвестно Рейтинг: Цена: 350370.00 T Наличие на складе: Невозможна поставка. Описание: This is the authoritative introduction to the concepts and techniques of recombinant DNA research and their dramatic results. The book explores core concepts in molecular biology in a contemporary inquiry-based context, building its coverage around the landmark experiments that redefined our understanding of DNA.
Автор: Venkatesh Balan Название: Microbial Lipid Production ISBN: 1493994867 ISBN-13(EAN): 9781493994861 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Нет в наличии.
Автор: Robert E. Rhoads Название: Synthetic mRNA ISBN: 1493936239 ISBN-13(EAN): 9781493936236 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy.
Автор: Venkatesh Balan Название: Microbial Lipid Production ISBN: 1493994832 ISBN-13(EAN): 9781493994830 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Нет в наличии. Описание:
The volume explores methods for isolation and characterization of lipids, and their production using various oleaginous organisms. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format. The book also discusses applications that use oleaginous organisms to transform substrates into a variety of products including bio-crude, high value fatty acids, biofuels such as biodiesel, neutral lipids, volatile fatty acids, and surfactants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Microbial Lipid Production: Methods and Protocols is a valuable resource aimed at novice researchers who are working on improving their knowledge and skills in this developing field.
Автор: S. Mohan Jain; S.K. Sopory; R.E. Veilleux Название: In Vitro Haploid Production in Higher Plants ISBN: 0792335775 ISBN-13(EAN): 9780792335771 Издательство: Springer Рейтинг: Цена: 256220.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This study covers historical and fundamental aspects of haploidy in crop improvement and deals with methods of haploid production, including anther culture, micropore culture, ovary culture, pollination with irradiated pollen, in vitro pollination and special culture techniques.
Автор: Shukla, Arun K. Название: Integrated Methods In Protein Biochemistry: Part A,675 ISBN: 0323992668 ISBN-13(EAN): 9780323992664 Издательство: Elsevier Science Рейтинг: Цена: 154960.00 T Наличие на складе: Есть у поставщика Поставка под заказ.
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