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Insoluble Proteins: Methods and Protocols, Garcia Fruitуs Elena, Arнs Giralt Anna


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Автор: Garcia Fruitуs Elena, Arнs Giralt Anna
Название:  Insoluble Proteins: Methods and Protocols
ISBN: 9781071618585
Издательство: Springer
Классификация:
ISBN-10: 107161858X
Обложка/Формат: Hardcover
Страницы: 600
Вес: 1.13 кг.
Дата издания: 27.10.2021
Язык: English
Размер: 25.91 x 18.29 x 3.30 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: Chapters describe recombinant protein production in different expression systems, prokaryotic and eukaryotic expression systems, purification protocols, characterization of insoluble proteins and a general overview of interesting applications of insoluble proteins.

Intrinsically Disordered Proteins: Methods and Protocols

Автор: Kragelund Birthe B., Skriver Karen
Название: Intrinsically Disordered Proteins: Methods and Protocols
ISBN: 1071605232 ISBN-13(EAN): 9781071605233
Издательство: Springer
Рейтинг:
Цена: 260870.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Intrinsically Disordered Proteins: Methods and Protocols aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules. Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com. Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

RNA Remodeling Proteins: Methods and Protocols

Автор: Boudvillain Marc
Название: RNA Remodeling Proteins: Methods and Protocols
ISBN: 1071609343 ISBN-13(EAN): 9781071609347
Издательство: Springer
Рейтинг:
Цена: 111790.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides the most current methods to study RNA remodeling proteins.

Cd95: Methods and Protocols

Автор: Legembre Patrick
Название: Cd95: Methods and Protocols
ISBN: 1493982982 ISBN-13(EAN): 9781493982981
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
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Описание:

Production of the Non-apoptotic Metalloprotease-cleaved CD95L and its Cytotoxic Recombinant Counterpart Designed Ig-CD95L.- CD95 Stimulation with CD95L and DISC Analysis.- Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8.- In vitro Evaluation of the Apoptosis Function in Human Activated T cells.- Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition.- Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization.- Generation and Application of Bioluminescent CD95 Ligand Fusion Proteins.- CD95-mediated Calcium Signaling.- CD95-mediated Proton Regulation.- Study of the CD95-mediated Non-apoptotic Signaling Pathway - PI3K.- Organelle Separation and Cell Signaling.- Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L.- Isolation of Lipid Rafts through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Receptor Localization in Raft Fractions.- Quantifying CD95/cl-CD95L Implications in Cell Mechanics And Membrane Tension by Atomic Force Microscopy Based Force Measurements.- Sketching of CD95 Oligomers by In Silico Investigations.- Site-specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventional and Phospho-Protein Affinity SDS-PAGE.- Detection of S-acylated CD95 by Acyl-biotin Exchange.- Exploration of Fas S-nitrosylation by the Biotin Switch Assay.- Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L.- Liquid Chromatography-High Resolution Mass Spectrometry Method to Study Sphingolipid Metabolism Changes in Response to CD95L.- CD95 and the MRL-lpr Mouse Model.


Insoluble Proteins

Автор: Elena Garc?a-Fruit?s
Название: Insoluble Proteins
ISBN: 1493946757 ISBN-13(EAN): 9781493946754
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples.

Argonaute Proteins: Methods and Protocols

Автор: Hobman Tom C., Duchaine Thomas F.
Название: Argonaute Proteins: Methods and Protocols
ISBN: 1493958275 ISBN-13(EAN): 9781493958276
Издательство: Springer
Рейтинг:
Цена: 135090.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.

The Tnf Superfamily: Methods and Protocols

Автор: Bayry Jagadeesh
Название: The Tnf Superfamily: Methods and Protocols
ISBN: 1071611291 ISBN-13(EAN): 9781071611296
Издательство: Springer
Рейтинг:
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This second edition focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Chapters detail protocols on the signaling process of TNF family members, technical examples to investigating the role of TNF family members in physiopathologies, modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, methodologies for various assays of TNF family members and the production of recombinant molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, The TNF Superfamily: Methods and Applications, Second Edition serves to aid researchers investigating this key family of proteins.

Protein Crystallography: Methods and Protocols

Автор: Wlodawer Alexander, Dauter Zbigniew, Jaskolski Mariusz
Название: Protein Crystallography: Methods and Protocols
ISBN: 1493983636 ISBN-13(EAN): 9781493983636
Издательство: Springer
Рейтинг:
Цена: 167700.00 T
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Описание:

1. Expression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag

Sreejith Raran-Kurussi and David S. Waugh

2. Protein Crystallization

Alexander McPherson

3. Advanced Methods of Protein Crystallization

Abel Moreno

4. The 'Sticky Patch' Model of Crystallization and Modification of Proteins for Enhanced Crystallizability

Zygmunt S. Derewenda and Adam Godzik

5. Crystallization of Membrane Proteins: and Overview

Andrii Ishchenko, Enrique Abola, and Vadim Cherezov

6. Locating and Visualizing Crystals for X-ray Diffraction Experiments

Michael Becker, David J. Kissick, and Craig M. Ogata

7. Collection of X-ray Diffraction Data from Macromolecular Crystals

Zbigniew Dauter

8. Identifying and Overcoming Crystal Pathologies: Disorder and Twinning

Michael C. Thompson

9. Applications of X-ray Micro-beam for Data Collection

Ruslan Sanishvili and Robert F. Fischetti

10. Serial Synchrotron X-ray Crystallography (SSX)

Kay Diederichs and Meitian Wang


11. Time-Resolved Macromolecular Crystallography at Modern X-ray Sources

Marius Schmidt

12. Structure Determination Using X-ray Free-electron Laser Pulses

Henry N. Chapman

13. Processing of XFEL Data

Thomas A. White

14. Many Ways to Derivatize Macromolecules and their Crystals for Phasing

Miroslawa Dauter and Zbigniew Dauter

15. Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX

Andrea Thorn

16. Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis

Qun Liu and Wayne A. Hendrickson

17. Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography

Manfred S. Weiss

18. Acknowledging Errors: Advanced Molecular Replacement with Phaser

Airlie J. McCoy

19. Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems

Frank DiMaio

20. Radiation Damage in Macromolecular Crystallography

Elspeth F. Garman and Martin Weik

21. Boxes of Model Building and Visualization

Dusan Turk

22. Structure Refinement at Atomic Resolution

Mariusz Jaskolski

23. Low Resolution Refinement of Atomic Models against Crystallographic Data

Robert A. Nicholls, Oleg Kovalevskiy, and Garib N. Murshudov

24. Stereochemistry and Validation of Macromolecular Structures

Alexander Wlodawer

25. Validation of Protein-Ligand Crystal Structure Models: Small Molecule and Peptide Ligands

Edwin Pozharski, Marc C. Deller and Bernhard Rupp

26. Protein Data Bank - the Single Global Macromolecular Structure Archive Managed by the Worldwide Protein Data Bank

Stephen K. Burley Helen M. Berman, Gerard J. Kleywegt, John L. Markley, Haruki Nakamura, and Sameer Velankar

27. Databases, Repositories and Other Data Resources in Structural Biology

Heping Zheng, Przemyslaw J. Porebski, Marek Grabowski, David R. Cooper, and Wladek Minor



Biophysics of Membrane Proteins: Methods and Protocols

Автор: Postis Vincent L. G., Goldman Adrian
Название: Biophysics of Membrane Proteins: Methods and Protocols
ISBN: 1071607235 ISBN-13(EAN): 9781071607237
Издательство: Springer
Цена: 211190.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Photoswitching Proteins: Methods and Protocols

Автор: Niopek Dominik
Название: Photoswitching Proteins: Methods and Protocols
ISBN: 107160757X ISBN-13(EAN): 9781071607572
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Нет в наличии.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Protein Synthesis: Methods and Protocols

Автор: Bhupendra Pushkar
Название: Protein Synthesis: Methods and Protocols
ISBN: 1774077035 ISBN-13(EAN): 9781774077030
Издательство: Mare Nostrum (Eurospan)
Рейтинг:
Цена: 151530.00 T
Наличие на складе: Поставка под заказ.
Описание: Takes readers through the various ways in which proteins are synthesized and then further used for other applications. The book throws light on chemical reactions taking place in the process, and lays down different kinds of methods and the procedures that are followed in protein synthesis.

Fe-S Proteins: Methods and Protocols

Автор: Dos Santos Patricia C.
Название: Fe-S Proteins: Methods and Protocols
ISBN: 1071616048 ISBN-13(EAN): 9781071616048
Издательство: Springer
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed book aims to provide an overview of novel bioinformatics resources for exploring diverse aspects of RNA biology. This edition focuses on methods dealing with non-coding RNA (miRNAs, circRNAs or lncRNAs), RNA modifications (m6A or RNA editing), single cell RNA-seq and statistical models to handle count data from RNA-seq experiments.

Ccn Proteins: Methods and Protocols

Автор: Takigawa Masaharu
Название: Ccn Proteins: Methods and Protocols
ISBN: 1493981978 ISBN-13(EAN): 9781493981977
Издательство: Springer
Рейтинг:
Цена: 116450.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques.


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