Gene cloning and dna analysis, Brown, Terry

Автор: Mei-Ling Ting Lee
Название: Analysis of Microarray Gene Expression Data
ISBN: 1475788231 ISBN-13(EAN): 9781475788235
Издательство: Springer
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Цена: 139750.00 T
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Описание: After genomic sequencing, microarray technology has emerged as a widely used platform for genomic studies in the life sciences. With the abundance of data produced from microarray studies, however, the ultimate impact of the studies on biology will depend heavily on data mining and statistical analysis.

Автор: Hardin
Название: Cloning Gene Expression
ISBN: 0195132947 ISBN-13(EAN): 9780195132946
Издательство: Oxford Academ
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Цена: 73920.00 T
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Описание: The central purpose of this combination lecture/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyzse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Electrophoresis, southern and western blotting and cominatorial techniques are empsized. Radioisotopes, fluorophores and solvent effects on protein structure are discussed. (No radioisotopes are required.) The experiments included have been selected because of their high success rate and because they emphasize a project-oriented approach. Sufficient detail and background are provided such that the intended audience includes advanced undergraduate students, beginning graduates, and practising professionals engaged in a broad range of pursuits. Each experiment is accompanied by detailed process rationale.

Автор: Patil Nayana, Sivaram Aruna
Название: A Complete Guide to Gene Cloning: From Basic to Advanced
ISBN: 3030968502 ISBN-13(EAN): 9783030968502
Издательство: Springer
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Цена: 111790.00 T
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Описание: This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.

Автор: Valdis Berzins
Название: Basic Cloning Procedures
ISBN: 364248977X ISBN-13(EAN): 9783642489778
Издательство: Springer
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Цена: 46570.00 T
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Автор: Bing-Yuan Chen; Harry W. Janes
Название: PCR Cloning Protocols
ISBN: 1617372811 ISBN-13(EAN): 9781617372810
Издательство: Springer
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Цена: 172350.00 T
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Описание: PCR Cloning Protocols, Second Edition, updates and expands Bruce White`s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.

Автор: Spangler
Название: Methods in Molecular Biology & Protein Chemistry - Cloning & Characterization of an Enterotoxin Subunit
ISBN: 0470843608 ISBN-13(EAN): 9780470843604
Издательство: Wiley
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Цена: 130890.00 T
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Описание: The advances made in molecular biology have allowed scientists to manipulate DNA. This means that molecular biology has become a tool for answering scientific questions that may be quite unrelated to genetics and cell biology. The techniques have become a means to an end, rather than an end in themselves. This work deals with this area.

Автор: Green, Michael R (Author), Sambrook, Joseph (Author)
Название: Molecular Cloning: A Laboratory Manual (Fourth Edition): Three-Volume Set (Revised)
ISBN: 1936113414 ISBN-13(EAN): 9781936113415
Издательство: INGRAM PUBLISHER SERVICES UK
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Цена: 0.00 T
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Описание: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator
Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other
leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include
nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression
analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays,
next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing
sequences of genes and proteins and identifying common expression patterns among sets of genes. Please take a look at our book preview site at molecularcloning.com. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the
one indispensable molecular biology laboratory manual and reference source.

Автор: Michael F. Ochs
Название: Gene Function Analysis
ISBN: 1617377481 ISBN-13(EAN): 9781617377488
Издательство: Springer
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Цена: 135090.00 T
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Описание: With the advent of high-throughput technologies following completion of the human genome project and similar projects, the number of genes of interest has expanded and the traditional methods for gene function analysis cannot achieve the throughput necessary for large-scale exploration.

Автор: K?re Lehmann Nielsen
Название: Serial Analysis of Gene Expression (SAGE)
ISBN: 1617377120 ISBN-13(EAN): 9781617377129
Издательство: Springer
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Цена: 111790.00 T
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Описание: This book facilitates the introduction of SAGE into the laboratory and provides a framework for interpreting and comparing data derived from SAGE experiments. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world.

Автор: Giovanni Parmigiani; Elizabeth S. Garett; Rafael A
Название: The Analysis of Gene Expression Data
ISBN: 1475781245 ISBN-13(EAN): 9781475781243
Издательство: Springer
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Цена: 139750.00 T
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Описание: This book presents practical approaches for the analysis of data from gene expression micro-arrays. The book includes coverage of various packages that are part of the Bioconductor project and several related R tools. The materials presented cover a range of software tools designed for varied audiences.

Автор: G.M. Addison; R. Angus Harkness; D.M Isherwood; R.
Название: Practical Developments in Inherited Metabolic Disease: DNA Analysis, Phenylketonuria and Screening for Congenital Adrenal Hyperplasia
ISBN: 9401083320 ISBN-13(EAN): 9789401083324
Издательство: Springer
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Цена: 81050.00 T
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Описание: The five case studies from Belize, Mauritania, Samoa, Philippines and Japan were prepared as part of a set of 16 studies gathering national experiences from around the world. The studies are intended to ground the FAO `Technical Guidelines on Marine Protected Areas (MPAs)` and `Fisheries` in practical experience and to inform the use of MPAs globally.

Автор: Hirak Ranjan Dash; Pankaj Shrivastava; Surajit Das
Название: Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing
ISBN: 107160273X ISBN-13(EAN): 9781071602737
Издательство: Springer
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Цена: 144410.00 T
Наличие на складе: Нет в наличии.
Описание: The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples. It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples. Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis. Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications.