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Cell Cycle Oscillators: Methods and Protocols, Coutts Amanda S., Weston Louise


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Автор: Coutts Amanda S., Weston Louise
Название:  Cell Cycle Oscillators: Methods and Protocols
ISBN: 9781071615379
Издательство: Springer
Классификация:
ISBN-10: 1071615378
Обложка/Формат: Hardcover
Страницы: 340
Вес: 0.77 кг.
Дата издания: 05.07.2021
Язык: English
Размер: 25.91 x 19.56 x 2.29 cm
Ссылка на Издательство: Link
Поставляется из: Германии
Описание: This book brings together a unique collection of protocols that cover novel and specialized techniques as well as updated and improved adaptations of more standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both holistic and reductionist perspectives.

Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
Рейтинг:
Цена: 13330.00 T
Наличие на складе: Есть
Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
Рейтинг:
Цена: 38730.00 T
Наличие на складе: Есть
Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Testicular Germ Cell Tumors: Methods and Protocols

Автор: Bagrodia Aditya, Amatruda James F.
Название: Testicular Germ Cell Tumors: Methods and Protocols
ISBN: 1071608622 ISBN-13(EAN): 9781071608623
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
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Описание: This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology;

Cell Penetrating Peptides: Methods and Protocols

Автор: Langel Ьlo
Название: Cell Penetrating Peptides: Methods and Protocols
ISBN: 1071617516 ISBN-13(EAN): 9781071617519
Издательство: Springer
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Цена: 167700.00 T
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Описание: This third edition summarizes the most important areas of Cell-Penetrating Peptides (CPP) research. Chapters are divided into three parts detailing the historical background of CPP, the classifications of the available CPPs, approaches for prediction of novel CPPs, methods for studies of “naked” CPPs, and a brief summary of functionality issues of CPPs, both in vitro and in vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Cell Penetrating Peptides: Methods and Protocols, Third Edition aims to be a useful practical guide to researches to help further their study in this field.

Cell Tracking: Methods and Protocols

Автор: Basel Matthew T., Bossmann Stefan H.
Название: Cell Tracking: Methods and Protocols
ISBN: 1071603639 ISBN-13(EAN): 9781071603635
Издательство: Springer
Рейтинг:
Цена: 139750.00 T
Наличие на складе: Поставка под заказ.

Plant Cell Division: Methods and Protocols

Автор: Caillaud Marie-Cйcile
Название: Plant Cell Division: Methods and Protocols
ISBN: 1071617435 ISBN-13(EAN): 9781071617434
Издательство: Springer
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Цена: 121110.00 T
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Описание: This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.

Stem Cell Heterogeneity: Methods and Protocols

Автор: Turksen Kursad
Название: Stem Cell Heterogeneity: Methods and Protocols
ISBN: 1493982346 ISBN-13(EAN): 9781493982349
Издательство: Springer
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Цена: 102480.00 T
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Описание: Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity.

3D Cell Culture: Methods and Protocols

Автор: Koledova Zuzana
Название: 3D Cell Culture: Methods and Protocols
ISBN: 1493983709 ISBN-13(EAN): 9781493983704
Издательство: Springer
Рейтинг:
Цена: 116450.00 T
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Описание: 1. 3D Cell Culture: An Introduction

Zuzana Koledova

Part I: Hydrogels and Scaffolds for 3D Cell Culture

2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture

Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest

3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix

Katrina Wisdom and Ovijit Chaudhuri

4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis

Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner

5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels

Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf

6. Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions

Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra

Part II: 3D Organoid and Organotypic Cultures

7. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells

Shinya Sugimoto and Toshiro Sato

8. 3D Co-Culture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis

Zuzana Koledova

9. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis

Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel

10. 3D Primary Culture Model to Study Human Mammary Development

Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta

11. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

Anas Rabata, Ales Hampl, and Zuzana Koledova

12. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids

Theresa Eder and Iris E. Eder

13. 3D Fluid-Dynamic Culture Models of Multiple Myeloma

Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero

14. Preparation of a Three-Dimensional Full Thickness Skin Equivalent

Christian Reuter, Heike Walles, and Florian Groeber

15. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System

Romana E. Ranftl and Fernando Calvo

16. 3D Co-Culture Model of the Brain Parenchyma/Metastasis Interface of Brain Metastasis

Raquel Blazquez and Tobias Pukrop

Part III: Micropatterning

Detection of Cell Death Mechanisms: Methods and Protocols

Автор: Alvero Ayesha B., Mor Gil G.
Название: Detection of Cell Death Mechanisms: Methods and Protocols
ISBN: 1071611615 ISBN-13(EAN): 9781071611616
Издательство: Springer
Рейтинг:
Цена: 158380.00 T
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Описание: This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type.

Cell cycle oscillators

Название: Cell cycle oscillators
ISBN: 1493929569 ISBN-13(EAN): 9781493929566
Издательство: Springer
Рейтинг:
Цена: 139750.00 T
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Cell Cycle Checkpoints: Methods and Protocols

Автор: Manfredi James J.
Название: Cell Cycle Checkpoints: Methods and Protocols
ISBN: 1071612166 ISBN-13(EAN): 9781071612163
Издательство: Springer
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Cell Cycle Synchronization: Methods and Protocols

Автор: Banfalvi Gaspar
Название: Cell Cycle Synchronization: Methods and Protocols
ISBN: 1493982486 ISBN-13(EAN): 9781493982486
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques.


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