DNA Cloning and Assembly, Sunil Chandran; Kevin W. George

Автор: P.H. Hofschneider; W. Goebel
Название: Gene Cloning in Organisms Other Than E. coli
ISBN: 3642683177 ISBN-13(EAN): 9783642683176
Издательство: Springer
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Описание: Gene cloning for the production of recombinant DNA is usually performed with E. coli. There is, however, no doubt that gene cloning in organisms other than E. coli will assume a much more important role in the future: efficient cloning systems are needed for the analysis of gene expression and its regulation in eukaryotic cells, for the elucidation ofthe genetic mechanisms of transform a- tion, and for the study of the genetic rearrangement during dif- ferentiation and embryogenesis. Furthermore, optimal cloning systems will be required for practical applications in the near future: for the optimal production of antibiotics, amino acids, vitamins, enzymes etc., for the expression of gene products under favorable energetic conditions for mass production, for the ex- pression of glycolysated animal proteins, for the genetic manipu- 'lation of plants -and in the more distant future, for gene therapy inman. The editors have therefore invited leading scientists to sum- marize the present status and the future applicability of gene cloning systems in their fields of research. To ensure a complete coverage of a genetic system, the editors have generally asked two authors to cover one system. This volume is a frrst and unique account of our knowledge of cloning systems employing organisms other than E. coli such as B. subtilis, pseudomonas, neurospora, saccharomyces and streptomyces, as well as of vectors and selection procedures for animal and plant systems including the liposome technique. October 1981 P. R.

Автор: Svein Valla; Rahmi Lale
Название: DNA Cloning and Assembly Methods
ISBN: 1493960571 ISBN-13(EAN): 9781493960576
Издательство: Springer
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Цена: 102480.00 T
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Описание: In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling.

Название: Dna cloning and assembly methods
ISBN: 1627037632 ISBN-13(EAN): 9781627037631
Издательство: Springer
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Цена: 158380.00 T
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Описание: In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling.

Автор: Seok-Yong Choi; Hyunju Ro; Hankuil Yi
Название: DNA Cloning: A Hands-on Approach
ISBN: 9402416609 ISBN-13(EAN): 9789402416602
Издательство: Springer
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Цена: 139750.00 T
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Описание: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

Автор: Sara El-Metwally; Osama M. Ouda; Mohamed Helmy
Название: Next Generation Sequencing Technologies and Challenges in Sequence Assembly
ISBN: 149390714X ISBN-13(EAN): 9781493907144
Издательство: Springer
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Цена: 51230.00 T
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Описание: The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.

Автор: Wong
Название: The ABCs of Gene Cloning
ISBN: 3319777629 ISBN-13(EAN): 9783319777627
Издательство: Springer
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Цена: 30740.00 T
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Описание: Clear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas. The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning.

Автор: David M. Glover
Название: Gene Cloning
ISBN: 0412266008 ISBN-13(EAN): 9780412266003
Издательство: Springer
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Цена: 104480.00 T
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Описание: This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA.It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size.I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion.Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop- ment ofother vectors has certainly not stood still over the past four years.This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.