Protein Tyrosine Phosphatases: Structure, Signaling and Drug Discovery, Ahuja Lalima G.

Автор: Pulido Rafael
Название: Protein Tyrosine Phosphatases: Methods and Protocols
ISBN: 1493981277 ISBN-13(EAN): 9781493981274
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.

Автор: Neel
Название: Protein Tyrosine Phosphatases in Cancer
ISBN: 1493936476 ISBN-13(EAN): 9781493936472
Издательство: Springer
Рейтинг:
Цена: 156720.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book aims to bridge the gap in understanding how protein-tyrosine phosphatases (PTPs), which carry out the reverse reaction of tyrosine phosphorylation, feature in cancer cell biology. The expertly authored chapters will first review the general features of the PTP superfamily, including their overall structure and enzymological properties; use selected examples of individual PTP superfamily members, to illustrate emerging data on the role of PTPs in cancer; and will review the current status of PTP-based drug development efforts.

Автор: Pulido
Название: Protein Tyrosine Phosphatases
ISBN: 1493937448 ISBN-13(EAN): 9781493937448
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Автор: Kendra K. Bence
Название: Protein Tyrosine Phosphatase Control of Metabolism
ISBN: 1489997989 ISBN-13(EAN): 9781489997982
Издательство: Springer
Рейтинг:
Цена: 139310.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume highlights many aspects of PTP function in the context of metabolism. It covers the identification of new phosphatases that are involved in metabolic control and includes a review of tissue-specific effects of protein tyrosine phosphatase 1B.

Автор: Deric L. Wheeler; Yosef Yarden
Название: Receptor Tyrosine Kinases: Structure, Functions and Role in Human Disease
ISBN: 1493920529 ISBN-13(EAN): 9781493920525
Издательство: Springer
Рейтинг:
Цена: 148020.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Receptor Tyrosine Kinase: Structure, Functions and Role in Human Disease, for the first time, systematically covers the shared structural and functional features of the RTK family.

Автор: Serena Germano
Название: Receptor Tyrosine Kinases
ISBN: 1493954490 ISBN-13(EAN): 9781493954490
Издательство: Springer
Рейтинг:
Цена: 88500.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

Part I: RTKs Activation and Signalling

1. Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblotting

Martina McDermott and Norma O'Donovan

2. Analysis of Changes in Phosphorylation of Receptor Tyrosine Kinases: Antibody Arrays

Sweta Rani and Lorraine O'Driscoll

3. Analysis of Epidermal Growth Factor Receptor Dimerization by BS³ Crosslinking

Harmony F. Turk and Robert S. Chapkin

4. Single-Molecule Optical Methods Analyzing Receptor Tyrosine Kinase Activation in Living Cells

Inhee Chung and Ira Mellman

5. Evaluation of the Dimerization Profiles of HER Tyrosine Kinases by Time-Resolved Fцrster Resonance Energy Transfer (TR-FRET)

Evelyne Lopez-Crapez, Alexandre Ho-Pun-Cheung, Patrick Garnero, and Hervй Bazin

6. Applying the Proximity Ligation Assay (PLA) to Mouse Preimplantation Embryos for Identifying Protein-Protein Interactions In Situ

Ivan Bedzhov and Marc P. Stemmler

Part II: RTKs Aberrant Expression

7. Analysis of Receptor Tyrosine Kinase Gene Amplification on the Example of FGFR1

Diana Boehm, Anne von Mдssenhausen, and Sven Perner

8. Quantification of the Effects of Mutations on Receptor Tyrosine Kinase (RTK) Activation in Mammalian Cells

Lijuan He and Kalina Hristova

Part III: RTKs Trafficking and Negative Regulation

9. Cell Surface Biotinylation of Receptor Tyrosine Kinases to Investigate Intracellular Trafficking

Mathieu J.F. Crupi, Douglas S. Richardson, and Lois M. Mulligan

10. Studying N-Linked Glycosylation of Receptor Tyrosine Kinases

Harri M. Itkonen and Ian G. Mills

11. Identification of Receptor Protein Tyrosine Phosphatases (RPTPs) as Regulators of Receptor Tyrosine Kinases (RTKs) Using an RPTP siRNA-RTK Substrate Screen

Hojin Lee and Anton M. Bennett

12. Downregulation of Receptor Tyrosine Kinases Through Ubiquitination: Analysis by Immunodetection

Noriaki Shimokawa and Noriyuki Koibuchi

13. Regulation of Receptor Tyrosine Kinases by miRNA: Overexpression of miRNA Using Lentiviral Inducible Expression Vectors

XiangDong Le, Andrew T. Huang, Yunyun Chen, and Stephen Y. Lai

Part IV: RTKs as Pharmacological Targets

14. Human Tumor Xenografts in Mouse as a Model for Evaluating Therapeutic Efficacy of Monoclonal Antibodies or Antibody-Drug Conjugate Targeting Receptor Tyrosine Kinases

Liang Feng, Wei Wang, Hang-Ping Yao, Jianwei Zhou, Ruiwen Zhang, and Ming-Hai Wang

15. Receptor Tyrosine Kinase Targeting in Multicellular Spheroids

Susan Breslin and Lorraine O'Driscoll

16. Receptor Tyrosine Kinases and Drug Resistance: Development and Characterization of In Vitro Models of Resistance to RTK Inhibitors

Claire Corcoran and Lorraine O'Driscoll

Автор: Serena Germano
Название: Receptor Tyrosine Kinases
ISBN: 1493917889 ISBN-13(EAN): 9781493917884
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Receptor Tyrosine Kinases: Methods and Protocols explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies. Beginning with a section on activation and signaling of Receptor Tyrosine Kinases (RTKs), the book continues with chapters on aberrant expression of RTKs, trafficking and negative regulation, as well as RTKs as pharmacological targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Tyrosine Kinases: Methods and Protocols contains an up-to-date overview of the most relevant and widely used methods employed in this field, providing detailed protocols that molecular and cellular biologists could easily adopt in their research programs on RTKs.