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Plant Chemical Genomics: Methods and Protocols, Hicks Glenn R., Zhang Chunhua


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Автор: Hicks Glenn R., Zhang Chunhua
Название:  Plant Chemical Genomics: Methods and Protocols
ISBN: 9781071609538
Издательство: Springer
Классификация:


ISBN-10: 107160953X
Обложка/Формат: Hardcover
Страницы: 196
Вес: 0.54 кг.
Дата издания: 04.01.2021
Серия: Methods in molecular biology
Язык: English
Издание: 2nd ed. 2021
Иллюстрации: 30 illustrations, color; 14 illustrations, black and white; xii, 196 p. 44 illus., 30 illus. in color.; 30 illustrations, color; 14 illustrations, bla
Размер: 25.91 x 19.56 x 1.52 cm
Читательская аудитория: Professional & vocational
Подзаголовок: Methods and protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: This second edition volume is a companion volume to the previous edition and looks at new findings on novel bioactive chemicals and cognate targets, as well as the use of synthetic small molecules and a variety of tools to understand these processes.

In Situ Detection of DNA Damage / Methods and Protocols

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 38730.00 T
Наличие на складе: Есть
Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Affinity Chromatography / Methods and Protocols

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 13330.00 T
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Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Plant Epigenetics and Epigenomics: Methods and Protocols

Автор: Spillane Charles, McKeown Peter
Название: Plant Epigenetics and Epigenomics: Methods and Protocols
ISBN: 1071601784 ISBN-13(EAN): 9781071601785
Издательство: Springer
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Цена: 111790.00 T
Наличие на складе: Нет в наличии.
Описание: This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.

Plant Epigenetics: Methods and Protocols

Автор: Kovalchuk Igor
Название: Plant Epigenetics: Methods and Protocols
ISBN: 1493979566 ISBN-13(EAN): 9781493979561
Издательство: Springer
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Цена: 88500.00 T
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Описание:

1. Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis

Wanhui You, Stйphane Pien, and Ueli Grossniklaus

2. Chromatin Conformation Capture-Based Analysis of Nuclear Architecture

Stefan Grob and Ueli Grossniklaus

3. Metaanalysis of Genome-Wide Chromatin Data

Julia Engelhorn and Franziska Turck

4. Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide Probes

Sara Hernбndez-Castellano, Geovanny I. Nic-Can, Clelia De-La-Peсa

5. COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns

Andriy Bilichak and Igor Kovalchuk

6. Analysis of Global Genome Methylation Using the Cytosine-Extension Assay

Andriy Bilichak and Igor Kovalchuk

7. In Situ Analysis Of DNA Methylation in Plants

Palak Kathiria and Igor Kovalchuk

8. Analysis of DNA Hydroxymethylation in Arabidopsis Using Colorimetric Assay

Andrey Golubov and Igor Kovalchuk

9. Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP)

Marнa Бngeles Guevara, Nuria De Marнa, Enrique Sбez-Laguna, Marнa Dolores Vйlez, Marнa Teresa Cervera, and Josй Antonio Cabezas

10. Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized Genomes

Pavlina Sasheva Ueli Grossniklaus

11. Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays

Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe, and Vincent Colot

12. Northern Blotting Techniques for Small RNAs

Todd Blevins

13. Stem-Loop QRT-PCR for the Detection of Plant Micrornas

Erika Varkonyi-Gasic

14. Profiling New Small RNA Sequences

Masayuki Tsuzuki and Yuichiro Watanabe

15. Small RNA Library Preparation and Illumina Sequencing in Plants

Andriy Bilichak, Andrey Golubov, and Igor Kovalchuk

16. Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow

Slava Ilnytskyy and Andriy Bilichak

17. Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression using Virus-Induced Gene Silencing

Andriy Bilichak and Igor Kovalchuk

18. The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks

Andriy Bilichak and Igor Kovalchuk

19. Profiling Transposable Elements and their Epigenetic Effects in Non-Model Species

Christian Parisod


Plant Hormones: Methods and Protocols

Автор: Kleine-Vehn Jьrgen, Sauer Michael
Название: Plant Hormones: Methods and Protocols
ISBN: 1493982109 ISBN-13(EAN): 9781493982103
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
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Описание:

Real Time Analysis of the Apical Hook Development.- Grafting with Arabidopsis thaliana.- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants.- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.- High throughput scoring of Seed Germination.- Histochemical Staining of β-Glucuronidase and its Spatial Quantification.- Imaging TCSn:: GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana.- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins.- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas.- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin.- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells.- In vivo Identification of Plant Protein Complexes using IP-MS/MS.- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons.- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).- Hormone Receptor Glycosylation.- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.- Hormone Profiling in Plant Tissues.- Use of Xenopus laevis Oocytes to Study Auxin Transport.- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.


Plant Embryogenesis: Methods and Protocols

Автор: Bayer Martin
Название: Plant Embryogenesis: Methods and Protocols
ISBN: 1071603418 ISBN-13(EAN): 9781071603413
Издательство: Springer
Рейтинг:
Цена: 111790.00 T
Наличие на складе: Нет в наличии.
Описание: This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species.

Plant Chemical Genomics

Автор: Fauser
Название: Plant Chemical Genomics
ISBN: 149397873X ISBN-13(EAN): 9781493978731
Издательство: Springer
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Цена: 144410.00 T
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Описание:

This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.

Plant Synthetic Promoters: Methods and Protocols

Автор: Hehl Reinhard
Название: Plant Synthetic Promoters: Methods and Protocols
ISBN: 1493981862 ISBN-13(EAN): 9781493981861
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.

Plant Kinases: Methods and Protocols

Автор: Dissmeyer Nico, Schnittger Arp
Название: Plant Kinases: Methods and Protocols
ISBN: 149395721X ISBN-13(EAN): 9781493957217
Издательство: Springer
Рейтинг:
Цена: 111790.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.

The Plant Microbiome: Methods and Protocols

Автор: Carvalhais Lilia C., Dennis Paul G.
Название: The Plant Microbiome: Methods and Protocols
ISBN: 1071610392 ISBN-13(EAN): 9781071610398
Издательство: Springer
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume in the Encyclopedia of Sustainability Science and Technology, Second Edition, describes the breadth of science and engineering knowledge critical to advancing sustainable built environments, from architecture and design, mechanical engineering, lighting, and materials to water and energy, public policy, and economics.

Biosecurity in Putin`s Russia

Автор: Zilinskas, Raymond A. Mauger, Philippe
Название: Biosecurity in Putin`s Russia
ISBN: 1626376980 ISBN-13(EAN): 9781626376984
Издательство: Mare Nostrum (Eurospan)
Рейтинг:
Цена: 100710.00 T
Наличие на складе: Невозможна поставка.
Описание: Ranging from the Soviet legacy to current doctrine, from advanced weapons-development networks to civilian biotechnology research, from diplomatic initiatives to disinformation campaigns, this book documents and analyses the build-up and modernization of Russia`s biodefense establishment under the Putin administration.

Plant Genomics: Methods and Protocols

Автор: Busch Wolfgang
Название: Plant Genomics: Methods and Protocols
ISBN: 1493983644 ISBN-13(EAN): 9781493983643
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Part I: Genotypes

1. CRISPR/Cas-mediated in planta Gene Targeting

Simon Schiml, Friedrich Fauser, and Holger Puchta

2. User Guide for the LORE1 Insertion Mutant Resource

Terry Mun, Anna Malolepszy, Niels Sandal, Jens Stougaard, and Stig U. Andersen

3. Enabling Reverse Genetics in Medicago truncatula Using High Throughput Sequencing for Tnt1 Flanking Sequence Recovery

Xiaofei Cheng, Nick Krom, Shulan Zhang, Kirankumar S. Mysore, Michael Udvardi, and Jiangqi Wen

4. Generation of Doubled Haploid Lines for QTL Mapping

Daniele L. Filiault, Danelle K. Seymour, Ravi Maruthachalam, and Julin N. Maloof

Part II: Phenotypes

5. Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing

Jцrg Hagmann and Claude Becker

6. Circular Chromosome Conformation Capture in Plants

Stefan Grob

7. Genome Wide Profiling of Histone Modifications and Histone Variants in Arabidopsis thaliana and Marchantia polymorpha

Ramesh Yelagandula, Akihisa Osakabe, Elin Axelsson, Frederic Berger, and Tomokazu Kawashima

8. Tissue-Specific Transcriptome Profiling in Arabidopsis Roots

Erin E. Sparks and Philip N. Benfey

9. Sample Preparation Protocols for Protein Abundance, Acetylome and Phosphoproteome Profiling of Plant Tissues

Gaoyuan Song, Maxwell R McReynolds, and Justin W Walley

10. Automated High-throughput Root Phenotyping of Arabidopsis thaliana under Nutrient Deficiency Conditions

Santosh B. Satbhai, Christian Göschl, and Wolfgang Busch

11. Large-scale Phenotyping of Root Traits in the Model Legume Lotus japonicus

Marco Giovannetti, Anna Malolepszy, Christian Goeschl, and Wolfgang Busch

12. Long-term Confocal Imaging of Arabidopsis thaliana Roots for Simultaneous Quantification of Root Growth and Fluorescent Signals

Delyana Stoeva, Christian Gцschl, and Wolfgang Busch

Part III: Molecular Bases of Phenotypes

13. Identification of Protein-DNA Interactions Using Enhanced Yeast One-Hybrid Assays and a Semi-Automated Approach

Allison Gaudinier, Michelle Tang, Anne-Maarit Bеgman, and Siobhan M. Brady

14. Mapping Protein- protein Interaction using High-throughput Yeast-2-Hybrid

Jessica Lopez and M. Shahid Mukhtar

15. Mapping Protein-protein Interactions using Affinity Purification and Mass Spectrometry

Chin-Mei Lee, Christopher Adamchek, Ann Feke, Dmitri A. Nusinow, and Joshua M. Gendron

16. Measuring Protein Movement, Oligomerization State, and Protein-Protein Interaction in Arabidopsis Roots Using Scanning Fluorescence Correlation Spectroscopy (scanning FCS)
Natalie M. Clark and Rosangela Sozzani

17. Studying Protein-Protein Interactions in planta using Advanced Fluorescence Microscopy

Marc Somssich and Rьdiger Simon

18. Chemiluminescence Based Detection of Peptide Activity and Peptide-Receptor Binding in Plants

Mari Wildhagen, Markus Albert, and Melinka A. Butenko

19. Application of Chemical Genomics to Plant-Bacteria Communication: A High-Throughput System to Identify Novel Molecules Modulating the Induction of Bacterial Virulence Genes by Plant Signals

Elodie Vandelle, Maria Rita Puttilli, Andrea Chini, Giulia Devescovi, Vittorio Venturi, and Annalisa Polverari



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