Heterologous Protein Production in Cho Cells: Methods and Protocols, Meleady Paula
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Автор: Paula Meleady Название: Heterologous Protein Production in CHO Cells ISBN: 1493969714 ISBN-13(EAN): 9781493969715 Издательство: Springer Цена: 139750 T Описание: This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using `omic approaches.
Автор: Paula Meleady Название: Heterologous Protein Production in CHO Cells ISBN: 1493969714 ISBN-13(EAN): 9781493969715 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using `omic approaches.
Автор: Kihara Daisuke Название: Protein Function Prediction: Methods and Protocols ISBN: 1493983687 ISBN-13(EAN): 9781493983681 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Preface... Table of Contents... Contributing Authors... 1. Using PFP and ESG Protein Function Prediction Web Servers Qing Wei, Joshua McGraw, Ishita Khan, and Daisuke Kihara 2. GHOSTX: A Fast Sequence Homology Search Tool for Functional Annotation of Metagenomic Data Shuji Suzuki, Takashi Ishida, Masahito Ohue, Masanori Kakuta, and Yutaka Akiyama 3. From Gene Annotation to Function Prediction for Metagenomics Fatemeh Sharifi and Yuzhen Ye 4. An Agile Functional Analysis of Metagenomic Data using SUPER-FOCUS Genivaldo Gueiros Z. Silva, Fabyano A. C. Lopes, and Robert A. Edwards 5. MPFit: Computational Tool for Predicting Moonlighting Proteins Ishita Khan, Joshua McGraw, and Daisuke Kihara 6. Predicting Secretory Proteins with SignalP Henrik Nielsen 7. The ProFunc Function Prediction Server Roman A. Laskowski 8. G-LoSA for Prediction of Protein-Ligand Binding Sites and Structures Hui Sun Lee andWonpil Im 9. Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning Michal Brylinski 10. WATsite2.0 with PyMOL Plugin: Hydration Site Prediction and Visualization Ying Yang, Bingjie Hu, and Markus A. Lill 11. Enzyme Annotation and Metabolic Reconstruction Using KEGG Minoru Kanehisa 12. Ortholog Identification and Comparative Analysis of Microbial Genomes using MBGD and RECOG Ikuo Uchiyama 13. Exploring Protein Function Using the Saccharomyces Genome Database Edith D. Wong 14. Network-Based Gene Function Prediction in Mouse and Other Model Vertebrates using MouseNet Server Eiru Kim and Insuk Lee 15. The FANTOM5 Computation Ecosystem: Genomic Information Hub for Promoters and Active Enhancers Imad Abugessaisa, Shuhei Noguchi, Piero Carninci, and Takeya Kasukawa 16. Multi-Algorithm Particle Simulations with Spatiocyte Satya N. V. Arjunan and Koichi Takahashi
Автор: Isabelle Mus-Veteau Название: Heterologous Expression of Membrane Proteins ISBN: 1493961322 ISBN-13(EAN): 9781493961320 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This book provides an overview of the different heterologous expression systems available to produce various membrane proteins for structural analysis. Chapters examine membrane protein solubilization, purification, and instability in solution.
Автор: Mus-Veteau Название: Heterologous Expression of Membrane Proteins ISBN: 1493936352 ISBN-13(EAN): 9781493936359 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.
Автор: Thomas C. Evans, Jr.; Ming-Qun Xu Название: Heterologous Gene Expression in E.coli ISBN: 1493957252 ISBN-13(EAN): 9781493957255 Издательство: Springer Рейтинг: Цена: 135090.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.
Автор: Vila-Perellу Miquel Название: Expressed Protein Ligation: Methods and Protocols ISBN: 1071604333 ISBN-13(EAN): 9781071604335 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Поставка под заказ. Описание: This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
Автор: Schueler-Furman, Ora;London, Nir Название: Modeling Peptide-Protein Interactions : Methods and Protocols ISBN: 1493967967 ISBN-13(EAN): 9781493967964 Издательство: Springer Рейтинг: Цена: 93160.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume covers an array of techniques available for studying peptide-protein docking and design.
Автор: Schueler-Furman Ora, London Nir Название: Modeling Peptide-Protein Interactions: Methods and Protocols ISBN: 1493983024 ISBN-13(EAN): 9781493983025 Издательство: Springer Рейтинг: Цена: 79190.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume covers an array of techniques available for studying peptide-protein docking and design. The book is divided into four sections: peptide binding site prediction; peptide-protein docking; prediction and design of peptide binding specificity; and the design of inhibitory peptides. The chapters in Modeling Peptide-Protein Interactions: Methods and Protocols cover topics such as the usage of ACCLUSTER and PeptiMap for peptide binding site prediction; AnchorDock and ATTRACT for blind, flexible docking of peptides to proteins; flexible peptide docking using HADDOCK and FlexPepDock; identifying loop-mediated protein-protein interactions using LoopFinder; and protein-peptide interaction design using PinaColada. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary details for successful application of the different approaches and step-by-step, readily reproducible protocols, as well as tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Modeling Peptide-Protein Interactions: Methods and Protocols provides a diverse and unified overview of this rapidly advancing field of major interest and applicability.
Автор: Walls Dermot, Loughran Sinйad T. Название: Protein Chromatography: Methods and Protocols ISBN: 1493961292 ISBN-13(EAN): 9781493961290 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
Автор: Abbott D. Wade, Lammerts Van Bueren Alicia Название: Protein-Carbohydrate Interactions: Methods and Protocols ISBN: 1493983326 ISBN-13(EAN): 9781493983322 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques.
Автор: Kister Alexander E. Название: Protein Supersecondary Structures: Methods and Protocols ISBN: 1493991604 ISBN-13(EAN): 9781493991600 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Chapters in this book discuss topics such as sequence and structural features of different SSS elements; software used for the automatic annotation of secondary structure elements in proteins; the new developments in secondary and SSS prediction and the modern approaches for energy landscape calculation; protein misfolding and amyloid structural formation; analysis of ‘transformer proteins’; discovery of the structure of the hydrophobic nucleus; and discussions of the main principles of protein structures formation. The contributing authors of the volume are the eminent experts in the field of protein research and bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Protein Supersecondary Structures: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about the relationship between amino acids sequences and protein structures, the evolution of proteins and the dynamics of protein formation.
Автор: Wlodawer Alexander, Dauter Zbigniew, Jaskolski Mariusz Название: Protein Crystallography: Methods and Protocols ISBN: 1493983636 ISBN-13(EAN): 9781493983636 Издательство: Springer Рейтинг: Цена: 167700.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Expression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag
Sreejith Raran-Kurussi and David S. Waugh
2. Protein Crystallization
Alexander McPherson
3. Advanced Methods of Protein Crystallization
Abel Moreno
4. The 'Sticky Patch' Model of Crystallization and Modification of Proteins for Enhanced Crystallizability
Zygmunt S. Derewenda and Adam Godzik
5. Crystallization of Membrane Proteins: and Overview
Andrii Ishchenko, Enrique Abola, and Vadim Cherezov
6. Locating and Visualizing Crystals for X-ray Diffraction Experiments
Michael Becker, David J. Kissick, and Craig M. Ogata
7. Collection of X-ray Diffraction Data from Macromolecular Crystals
Zbigniew Dauter
8. Identifying and Overcoming Crystal Pathologies: Disorder and Twinning
Michael C. Thompson
9. Applications of X-ray Micro-beam for Data Collection
Ruslan Sanishvili and Robert F. Fischetti
10. Serial Synchrotron X-ray Crystallography (SSX)
Kay Diederichs and Meitian Wang
11. Time-Resolved Macromolecular Crystallography at Modern X-ray Sources
Marius Schmidt
12. Structure Determination Using X-ray Free-electron Laser Pulses
Henry N. Chapman
13. Processing of XFEL Data
Thomas A. White
14. Many Ways to Derivatize Macromolecules and their Crystals for Phasing
Miroslawa Dauter and Zbigniew Dauter
15. Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX
Andrea Thorn
16. Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis
Qun Liu and Wayne A. Hendrickson
17. Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography
Manfred S. Weiss
18. Acknowledging Errors: Advanced Molecular Replacement with Phaser
Airlie J. McCoy
19. Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems
Frank DiMaio
20. Radiation Damage in Macromolecular Crystallography
Elspeth F. Garman and Martin Weik
21. Boxes of Model Building and Visualization
Dusan Turk
22. Structure Refinement at Atomic Resolution
Mariusz Jaskolski
23. Low Resolution Refinement of Atomic Models against Crystallographic Data
Robert A. Nicholls, Oleg Kovalevskiy, and Garib N. Murshudov
24. Stereochemistry and Validation of Macromolecular Structures
Alexander Wlodawer
25. Validation of Protein-Ligand Crystal Structure Models: Small Molecule and Peptide Ligands
Edwin Pozharski, Marc C. Deller and Bernhard Rupp
26. Protein Data Bank - the Single Global Macromolecular Structure Archive Managed by the Worldwide Protein Data Bank
Stephen K. Burley Helen M. Berman, Gerard J. Kleywegt, John L. Markley, Haruki Nakamura, and Sameer Velankar
27. Databases, Repositories and Other Data Resources in Structural Biology
Heping Zheng, Przemyslaw J. Porebski, Marek Grabowski, David R. Cooper, and Wladek Minor
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