Stem Cells and Tissue Repair: Methods and Protocols, Kioussi Chrissa

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 13330.00 T
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Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Didenko Vladimir V.
Название: In Situ Detection of DNA Damage / Methods and Protocols
ISBN: 0896039528 ISBN-13(EAN): 9780896039520
Издательство: Springer
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Цена: 38730.00 T
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Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

Автор: Chrissa Kioussi
Название: Stem Cells and Tissue Repair
ISBN: 1493955322 ISBN-13(EAN): 9781493955329
Издательство: Springer
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Цена: 93160.00 T
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Описание:

1. Culturing and Differentiating Mouse Embryonic Stem Cells

Axel P. Gross and Chrissa Kioussi

2. Neural Stem Cells Transplantation in an Animal Model of Traumatic Brain Injury

Dimitra Thomaidou

3. Experimental Cell Transplantation for Traumatic Spinal Cord Injury Regeneration: Intramedullar or Intrathecal Administration

Ana Alastrue-Agudo, Slaven Erceg, Marta Cases-Villar, Viviana Bisbal-Velasco, Richard J. Griffeth, Francisco Javier Rodriguez-Jimйnez, and Victoria Moreno-Manzano

4. Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for in vivo Analysis of the Brain Tumor Initiating Cell

Maleeha Qazi, Aneet Mann, Randy van Ommeren, Chitra Venugopal, Nicole McFarlane, Parvez Vora, and Sheila K. Singh

5. Growth of Bone Marrow and Skeletal Muscle Side Population Stem Cells in Suspension Culture

Christina A. Pacak and Douglas B. Cowan

6. Isolation, Culture and Immunostaining of Skeletal Muscle Fibers to Study Myogenic Progression in Satellite Cells

Louise A. Moyle and Peter S. Zammit

7. Human Neural Crest Stem Cells Derived from Human Pluripotent Stem Cells

Qiuyue Liu, Andrzej Swistowski, and Xianmin Zeng

8. Dental Pulp Stem Cells (DPSC) Isolation, Characterization and Differentiation

Federico Ferro, Renza Spelat, and Chelsea S. Baheney

9. Dental Pulp Stem Cells Isolation and Osteogenic Differentiation: A Good Promise for Tissue Engineering

Adriana Di Benedetto, Claudia Carbone, and Giorgio Mori

10. Efficient Hepatic Differentiation of Human Induced Pluripotent Stem Cells in A Three-Dimensional Microscale Culture

Ran-Ran Zhang, Takanori Takebe, Leina Miyazaki, Maho Takayama, Hiroyuki Koike, Masaki Kimura, Masahirop Enomura, Yun-Wen Zheng, Keisuke Sekine, and Hideki Taniguchi

11. The Generation and Maintenance of Rat Induced Pluripotent Stem Cells

Tomoyuki Yamaguchi, Sanae Hamanaka, and Hiromitsu Nakauchi

12. Protocol for Cutaneous Wound Healing Assay in a Murine Model

Gitali Ganguli-Indra

13. Adipose Derived Stem Cells: Methods for Isolation and Applications for Clinical Use

Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok, Anne M. Wallace, and Steven Cohen

14. In vitro Detection of Residual Undifferentiated Cells in Retinal Pigment Epithelial Cells Derived from Human Induced Pluripotent Stem Cells

Takuya Kuroda, Satoshi Yasuda, and Yoji Sato

15. Whole Mount Immunohistochemistry to Study Spermatological Stem Cells and Spermatogenic Lineage in Mice, Monkeys and Humans

Kathrin Gassei, Hanna Valli, and Kyle E. Orwig

16. Differentiating the Stem Cell Pool of Human Hair Follicle Outer Root Sheath into Functional Melanocytes

Marie Schneider, Christina Dieckmann, Kathrin Rabe, Jan-Christoph Simon, and Vuk Savkovic

17. Pancreas Development ex vivo: Culturing Embryonic Pancreas Explants on Permeable Culture Inserts, with Firbonectin-Coated Glass Microwells, or Embedded in Three-Dimensional MatrigelTM

Hung Ping Shih and Maike Sander

18. Ultra-Rapid Manufacturing of Engineered Epicardial Substitute to Regenerate Cardiac Tissue Following Acute Ischemic Injury

Vahid Serpooshan and Pilar Ruiz-Lozano

Автор: Goswami Ritobrata
Название: Th9 Cells: Methods and Protocols
ISBN: 1493983261 ISBN-13(EAN): 9781493983261
Издательство: Springer
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Цена: 130430.00 T
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Описание: The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model.

Автор: Amarnath Shoba
Название: Innate Lymphoid Cells: Methods and Protocols
ISBN: 107160337X ISBN-13(EAN): 9781071603376
Издательство: Springer
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Цена: 102480.00 T
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Автор: Turksen Kursad
Название: Epidermal Cells: Methods and Protocols
ISBN: 1071602500 ISBN-13(EAN): 9781071602508
Издательство: Springer
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Цена: 121110.00 T
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Описание: This fully updated edition brings together a new set of protocols to arm epidermal cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory mechanisms underlying epidermal cell development and function.

Автор: Turksen Kursad
Название: Stem Cell Heterogeneity: Methods and Protocols
ISBN: 1493982346 ISBN-13(EAN): 9781493982349
Издательство: Springer
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Цена: 102480.00 T
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Описание: Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity.

Автор: Turksen Kursad
Название: Stem Cell Nanotechnology: Methods and Protocols
ISBN: 1071603590 ISBN-13(EAN): 9781071603598
Издательство: Springer
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Цена: 102480.00 T
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Описание: This fully updated edition brings together a new set of protocols to arm stem cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory circuits underlying stem cell biology. The collection explores the tremendous progress in our understanding of the potential of stem cells and the regulatory mechanisms controlling their fate, particularly through the parallel advances in nanotechnologies and their applications in biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Nanotechnology: Methods and Protocols, Second Edition serves a valuable tool for experts and novices alike in the vital field of stem cell research.

Автор: Kidder Benjamin L.
Название: Stem Cell Transcriptional Networks: Methods and Protocols
ISBN: 1071603000 ISBN-13(EAN): 9781071603000
Издательство: Springer
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Цена: 111790.00 T
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Описание: This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, protocols on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks.

Автор: Narine Sarvazyan
Название: Tissue Engineering: Principles, Protocols, and Practical Exercises
ISBN: 3030396975 ISBN-13(EAN): 9783030396978
Издательство: Springer
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Цена: 69870.00 T
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Описание: Tissue engineering and regenerative medicine is a new, interdisciplinary branch of science that combines knowledge from numerous scientific fields including biology, biochemistry, physics, chemistry, applied engineering, and medicine. It aims to restore damaged parts of the human body by rebuilding them in vitro using individual building blocks of biological tissues such as cells and the extracellular matrix that surrounds them. The authors hope to spark students’ interest in this exciting new field of science as well as give them a basic knowledge of its terminology. This book is based on a hands-on practical course in tissue engineering conducted by the Fulbright US Scholar recipient, Dr. Narine Sarvazyan (George Washington University, Washington USA). It provides an overview of the core topics of the tissue engineering field, including stem cell differentiation, the role of extracellular matrix and attachment proteins, scaffolds, and culturing of engineered tissues. Each chapter is accompanied by hands-on demonstrations and self-check questions. The text is easily readable for students of all backgrounds and the described protocols can be conducted using common lab equipment. This textbook is also useful for developing undergraduate and graduate courses that teach basic methods and approaches in this promising and rapidly developing field.

Автор: Conboy Irina M., Schaffer David V., Barcellos-Hoff Mary Helen
Название: Protocols for Adult Stem Cells
ISBN: 1493961047 ISBN-13(EAN): 9781493961047
Издательство: Springer
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Цена: 102480.00 T
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Описание: Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body’s natural ability to fght disease, heal and recover, or fail and succumb to various maladies. The study of mammalian adult stem cells has surged recently, most likely from a maturation of stem cell studies in the classical developmental model organisms and in hematopoeisis. All the tissues of the body examined so far are generated and regenerated from stem cells, it has been an important frst step to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Much of this work, and that in the classical model org- isms, has helped defne the aspects of the stem cell environment or niche that are crucial for both growth and differentiation, and these studies have moved in vivo at increasingly higher resolution. Importantly, the in vivo niche is a current target for bioengineering the matrix and signaling factors. Herein, we present methods for studying six types of mammalian stem cells, m- mary, neural, mesenchymal, endothelial, dendritic, and muscle.

Автор: Daryl S. Henderson
Название: DNA Repair Protocols: Eukaryotic Systems (Methods in Molecular Biology)
ISBN: 1468471449 ISBN-13(EAN): 9781468471441
Издательство: Springer
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Цена: 148020.00 T
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