Plant Cytogenetics: Methods and Protocols, Kianian Shahryar F., Kianian Penny M. Avoles
Автор: Kianian Название: Plant Cytogenetics ISBN: 1493936204 ISBN-13(EAN): 9781493936205 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume covers arange of methods used in plant cytogenetics, beginning with basic analysis ofchromosomes and visualizing gene locations, to manipulating and dissectingchromosomes, and then focusing on less understood features of chromosomes suchas recombination initiation sites and epigenomic marks. The methods describedin Plant Cytogenetics: Methods and Protocols build on each other andprovide, those new to the field, with a comprehensive platform to support theirresearch endeavours, while also introducing advanced techniques to experiencedresearchers. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Cuttingedge and thorough, Plant Cytogenetics: Methods and Protocols, is avaluable resource for anyone who is interested in the diverse and wonderfullycomplex field of cytogenetics.
Автор: Yung-I Lee; Edward Chee-Tak Yeung Название: Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols ISBN: 1493992805 ISBN-13(EAN): 9781493992805 Издательство: Springer Рейтинг: Цена: 232910.00 T Наличие на складе: Поставка под заказ. Описание: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.
This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation. The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one’s enjoyment.
Автор: J. Sybenga Название: Cytogenetics in Plant Breeding ISBN: 364284085X ISBN-13(EAN): 9783642840852 Издательство: Springer Рейтинг: Цена: 121890.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: An introductory discussion of basic chromosome structure andfunction preceeds the main text on the application ofcytogenetic approaches to the analysis of the manipulationof both the genetic make-up and the genetic transmissionsystem of plant breeding material.
Автор: Hank Bass; James A. Birchler Название: Plant Cytogenetics ISBN: 1489986790 ISBN-13(EAN): 9781489986795 Издательство: Springer Рейтинг: Цена: 174130.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This reference book provides a unique combination of historical and modern subject matter, revealing the central role of plant cytogenetics in plant genetics and genomics as currently practiced. This breadth of coverage, together with the inclusion of methods and instruction, is intended to convey a deep and useful appreciation for the plant cytogenetics.
Автор: Fett-Neto Arthur Germano Название: Biotechnology of Plant Secondary Metabolism: Methods and Protocols ISBN: 1493980408 ISBN-13(EAN): 9781493980406 Издательство: Springer Рейтинг: Цена: 106010.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: 1. Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, and Markandan Manickavasagan 2. Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction Thanise Nogueira, Fьller, Jъlio Cйsar de Lima, Fernanco de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 3. A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines Jъlio Cйsar de Lima, Thanise Nogueira Fьller, Fernanda de Costa, Kelly C.S. Rodrigues-Corrкa, and Arthur G. Fett-Neto 4. Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 5. From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica Fernando de Costa, Carla J.S. Barber, Darwin W. Reed, and Patrick S. Covello 6. Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli Flбvia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera 7. Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala Vishal Singh Negi and Dulal Borthakur 8. Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast Anita Emmerstorfer and Harald Pichler 9. Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC) Fernanda de Costa, Carla J.S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, and Patrick S. Covello 10. A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes Morten Egevang Jrgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier 11. Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters Mбrcia R. de Almeida and Martina V. Strцmvik
12. Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants Inкs Carqueijeiro, Viviana Martins, Henrique Noronha, Hernвni Gerуs, and Mariana Sottomayor 13. Protoplast Transformation as a Plant-Transferable Transient Expression System Patrнcia Duarte, Diana Ribeiro, Inкs Carqueijeiro, Sara Bettencourt, and Mariana Sottomayor
14. Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System Basdeo Bhagwat, Ming Chi, Dianwei Han, Haifeng Tang, Guiliang Tang, and Yu Xiang
15. Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs Ming Chi, Basdeo Bhagwat, Guiliang Tang, and Yu Xiang
Автор: Shan Libo, He Ping Название: Plant Pattern Recognition Receptors: Methods and Protocols ISBN: 1493983202 ISBN-13(EAN): 9781493983209 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools.
Автор: Dissmeyer Nico, Schnittger Arp Название: Plant Kinases: Methods and Protocols ISBN: 149395721X ISBN-13(EAN): 9781493957217 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Автор: Hehl Reinhard Название: Plant Synthetic Promoters: Methods and Protocols ISBN: 1493981862 ISBN-13(EAN): 9781493981861 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.
Автор: Bayer Martin Название: Plant Embryogenesis: Methods and Protocols ISBN: 1071603418 ISBN-13(EAN): 9781071603413 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Поставка под заказ. Описание: This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species.
Автор: Kleine-Vehn Jьrgen, Sauer Michael Название: Plant Hormones: Methods and Protocols ISBN: 1493982109 ISBN-13(EAN): 9781493982103 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Real Time Analysis of the Apical Hook Development.- Grafting with Arabidopsis thaliana.- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants.- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.- High throughput scoring of Seed Germination.- Histochemical Staining of β-Glucuronidase and its Spatial Quantification.- Imaging TCSn:: GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana.- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins.- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas.- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin.- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells.- In vivo Identification of Plant Protein Complexes using IP-MS/MS.- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons.- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).- Hormone Receptor Glycosylation.- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.- Hormone Profiling in Plant Tissues.- Use of Xenopus laevis Oocytes to Study Auxin Transport.- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.
Автор: Kovalchuk Igor Название: Plant Epigenetics: Methods and Protocols ISBN: 1493979566 ISBN-13(EAN): 9781493979561 Издательство: Springer Рейтинг: Цена: 88500.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis
Wanhui You, Stйphane Pien, and Ueli Grossniklaus
2. Chromatin Conformation Capture-Based Analysis of Nuclear Architecture
Stefan Grob and Ueli Grossniklaus
3. Metaanalysis of Genome-Wide Chromatin Data
Julia Engelhorn and Franziska Turck
4. Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide Probes
Sara Hernбndez-Castellano, Geovanny I. Nic-Can, Clelia De-La-Peсa
5. COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns
Andriy Bilichak and Igor Kovalchuk
6. Analysis of Global Genome Methylation Using the Cytosine-Extension Assay
Andriy Bilichak and Igor Kovalchuk
7. In Situ Analysis Of DNA Methylation in Plants
Palak Kathiria and Igor Kovalchuk
8. Analysis of DNA Hydroxymethylation in Arabidopsis Using Colorimetric Assay
Andrey Golubov and Igor Kovalchuk
9. Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP)
Marнa Бngeles Guevara, Nuria De Marнa, Enrique Sбez-Laguna, Marнa Dolores Vйlez, Marнa Teresa Cervera, and Josй Antonio Cabezas
10. Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized Genomes
Pavlina Sasheva Ueli Grossniklaus
11. Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays
Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe, and Vincent Colot
12. Northern Blotting Techniques for Small RNAs
Todd Blevins
13. Stem-Loop QRT-PCR for the Detection of Plant Micrornas
Erika Varkonyi-Gasic
14. Profiling New Small RNA Sequences
Masayuki Tsuzuki and Yuichiro Watanabe
15. Small RNA Library Preparation and Illumina Sequencing in Plants
Andriy Bilichak, Andrey Golubov, and Igor Kovalchuk
16. Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow
Slava Ilnytskyy and Andriy Bilichak
17. Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression using Virus-Induced Gene Silencing
Andriy Bilichak and Igor Kovalchuk
18. The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks
Andriy Bilichak and Igor Kovalchuk
19. Profiling Transposable Elements and their Epigenetic Effects in Non-Model Species
Christian Parisod
Автор: Busch Wolfgang Название: Plant Genomics: Methods and Protocols ISBN: 1493983644 ISBN-13(EAN): 9781493983643 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Part I: Genotypes
1. CRISPR/Cas-mediated in planta Gene Targeting
Simon Schiml, Friedrich Fauser, and Holger Puchta
2. User Guide for the LORE1 Insertion Mutant Resource
Terry Mun, Anna Malolepszy, Niels Sandal, Jens Stougaard, and Stig U. Andersen
3. Enabling Reverse Genetics in Medicago truncatula Using High Throughput Sequencing for Tnt1 Flanking Sequence Recovery
Xiaofei Cheng, Nick Krom, Shulan Zhang, Kirankumar S. Mysore, Michael Udvardi, and Jiangqi Wen
4. Generation of Doubled Haploid Lines for QTL Mapping
Daniele L. Filiault, Danelle K. Seymour, Ravi Maruthachalam, and Julin N. Maloof
Part II: Phenotypes
5. Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing
Jцrg Hagmann and Claude Becker
6.Circular Chromosome Conformation Capture in Plants
Stefan Grob
7. Genome Wide Profiling of Histone Modifications and Histone Variants in Arabidopsis thaliana and Marchantia polymorpha
8. Tissue-Specific Transcriptome Profiling in Arabidopsis Roots
Erin E. Sparks and Philip N. Benfey
9. Sample Preparation Protocols for Protein Abundance, Acetylome and Phosphoproteome Profiling of Plant Tissues
Gaoyuan Song, Maxwell R McReynolds, and Justin W Walley
10. Automated High-throughput Root Phenotyping of Arabidopsis thaliana under Nutrient Deficiency Conditions
Santosh B. Satbhai, Christian Göschl, and Wolfgang Busch
11. Large-scale Phenotyping of Root Traits in the Model Legume Lotus japonicus
Marco Giovannetti, Anna Malolepszy, Christian Goeschl, and Wolfgang Busch
12. Long-term Confocal Imaging of Arabidopsis thaliana Roots for Simultaneous Quantification of Root Growth and Fluorescent Signals
Delyana Stoeva, Christian Gцschl, and Wolfgang Busch
Part III: Molecular Bases of Phenotypes
13. Identification of Protein-DNA Interactions Using Enhanced Yeast One-Hybrid Assays and a Semi-Automated Approach
Allison Gaudinier, Michelle Tang, Anne-Maarit Bеgman, and Siobhan M. Brady
14. Mapping Protein- protein Interaction using High-throughput Yeast-2-Hybrid
Jessica Lopez and M. Shahid Mukhtar
15.Mapping Protein-protein Interactions using Affinity Purification and Mass Spectrometry
Chin-Mei Lee, Christopher Adamchek, Ann Feke, Dmitri A. Nusinow, and Joshua M. Gendron
16. Measuring Protein Movement, Oligomerization State, and Protein-Protein Interaction in Arabidopsis Roots Using Scanning Fluorescence Correlation Spectroscopy (scanning FCS) Natalie M. Clark and Rosangela Sozzani
17.Studying Protein-Protein Interactions in planta using Advanced Fluorescence Microscopy
Marc Somssich and Rьdiger Simon
18. Chemiluminescence Based Detection of Peptide Activity and Peptide-Receptor Binding in Plants
Mari Wildhagen, Markus Albert, and Melinka A. Butenko
19. Application of Chemical Genomics to Plant-Bacteria Communication: A High-Throughput System to Identify Novel Molecules Modulating the Induction of Bacterial Virulence Genes by Plant Signals
Elodie Vandelle, Maria Rita Puttilli, Andrea Chini, Giulia Devescovi, Vittorio Venturi, and Annalisa Polverari
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