ATM Kinase: Methods and Protocols, Kozlov Sergei V.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 38730.00 T Наличие на складе: Есть Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 13330.00 T Наличие на складе: Есть Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Kwok Pui-Yan Название: Single Nucleotide Polymorphisms / Methods and Protocols ISBN: 0896039684 ISBN-13(EAN): 9780896039681 Издательство: Springer Цена: 38730.00 T Наличие на складе: Заказано в издательстве. Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Автор: Didenko Vladimir V. Название: Fast Detection of DNA Damage: Methods and Protocols ISBN: 149398411X ISBN-13(EAN): 9781493984114 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.
Автор: Azorsa David O., Arora Shilpi Название: High-Throughput Rnai Screening: Methods and Protocols ISBN: 1493981684 ISBN-13(EAN): 9781493981687 Издательство: Springer Рейтинг: Цена: 93160.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries. The success of these screening efforts is dependent on robust methodologies to perform these screens. In High-Throughput RNAi Screening: Methods and Protocols, expert researchers in the field share protocols and methods for performing high-throughput RNAi (HT-RNAi) screens. These include the use of various RNAi platforms and delivery methods in mammalian and non-mammalian systems, whole organism and cell models, and various applications, such as drug sensitizer identification. Finally, the book examines the latest advancements in the fields of assay development, library screening, data analysis, and hit selection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, High-Throughput RNAi Screening: Methods and Protocols provides a comprehensive source of protocols and other necessary information to make robust and successful assays possible for all who wish to apply HT-RNAi in their research.
Автор: Magnus Lundgren Название: CRISPR : methods and protocols ISBN: 1493926861 ISBN-13(EAN): 9781493926862 Издательство: Springer Рейтинг: Цена: 158380.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.
Автор: Grutzmann Robert, Pilarsky Christian Название: Cancer Gene Profiling: Methods and Protocols ISBN: 1493979973 ISBN-13(EAN): 9781493979974 Издательство: Springer Рейтинг: Цена: 106650.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Factors Affecting the Use of Human Tissues in Biomedical Research: Implications in the Design and Operation of a Biorepository Daniel S. Atherton, Katherine C. Sexton, Dennis Otali, Walter C. Bell, and William E. Grizzle
2. Tissue Microdissection Anja Rabien and Glen Kristiansen
3. Tissue Microarrays Ana-Maria Dancau, Ronald Simon, Martina Mirlacher, and Guido Sauter
4. Gene Expression Analysis in the Age of Mass Sequencing: An Introduction Christian Pilarsky, Lahiri Kanth Nanduri, and Janine Roy
5. Quantitative DNA Methylation in Cancer Ole Ammerpohl, Andrea Haake, Julia Kolarova, and Reiner Siebert
6. Single Nucleotide Polymorphism to Associate Cancer Risk Victoria Shaw, Katie Bullock, and William Greenhalf
7: Exosomes in Cancer Disease Margot Zцller
8: MicroRNAs (miRNAs) Profiling Lu Gao and Feng Jiang
9. Cancer Gene Profiling for Response Prediction B. Michael Ghadimi and Peter Jo
10. Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment Sanjay Tiwari, Ole Ammerpohl, and Holger Kalthoff
11. Application of Proteomics in Cancer Biomarker Discovery: GeLC-MS/MS Pedro R. Cutillas and Tatjana Crnogorac-Jurcevic
12. Meta-Analysis of Cancer Gene Profiling Data Janine Roy, Christof Winter, and Michael Schroeder
13. Reproducible, Scalable Fusion Gene Detection from RNA-Seq Vladan Arsenijevic and Brandi N. Davis-Dusenbery
14. Transcriptome Sequencing for the Detection of Chimeric Transcripts Hsueh-Ting Chu
Автор: Mathй Ewy, Davis Sean Название: Statistical Genomics: Methods and Protocols ISBN: 1493980831 ISBN-13(EAN): 9781493980833 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume expands on statistical analysis of genomic data by discussing cross-cutting groundwork material, public data repositories, common applications, and representative tools for operating on genomic data. Statistical Genomics: Methods and Protocols is divided into four sections. The first section discusses overview material and resources that can be applied across topics mentioned throughout the book. The second section covers prominent public repositories for genomic data. The third section presents several different biological applications of statistical genomics, and the fourth section highlights software tools that can be used to facilitate ad-hoc analysis and data integration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step-by-step, readily reproducible analysis protocols, and tips on troubleshooting and avoiding known pitfalls. Through and practical, Statistical Genomics: Methods and Protocols, explores a range of both applications and tools and is ideal for anyone interested in the statistical analysis of genomic data.
Автор: William S. M. Wold; Ann E. Tollefson Название: Adenovirus Methods and Protocols ISBN: 1617378356 ISBN-13(EAN): 9781617378355 Издательство: Springer Рейтинг: Цена: 149060.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. Each of the protocols is written by trendsetting researchers.
Автор: Sergei V. Kozlov Название: ATM Kinase ISBN: 1493969536 ISBN-13(EAN): 9781493969531 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Assaying Radiosensitivity of Ataxia-Telangiectasia
Hailiang Hu, Shareef Nahas, and Richard A. Gatti
2. Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Fluorography-Based Methodology
Amanda W. Kijas and Martin F. Lavin
3. ATM Gene Mutation Detection Techniques and Functional Analysis
Guillaume Rieunier, Catherine Dubois D'Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, and Marc-Henri Stern
4. An HTRF(R) Assay for the Protein Kinase ATM
Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, and Andrew Plater
6. Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci
Yi Chieh Lim
7. Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy
Keiji Suzuki
8. Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging
Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla
9. Zn(II)-Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein
Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
10. Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry
Mark E. Graham, Martin F. Lavin, and Sergei V. Kozlov
11. Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS)
Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, and Ryo Otomo
12. Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies
Sun Yingli and Du Fengxia
13. Identification of ATM-Interacting Proteins by Co-Immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays
Amanda L. Bain, Janelle L. Harris, and Kum Kum Khanna
14. ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning-Cytometry
Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, and Zbigniew Darzynkiewicz
15. Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling
Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, and Amanda G. Paulovich
16. Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosph
Автор: Salomons Название: Creatine and Creatine Kinase in Health and Disease ISBN: 9048176522 ISBN-13(EAN): 9789048176526 Издательство: Springer Рейтинг: Цена: 214290.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume places emphasis on the intricate interplay between creatine and creatine kinase function on one hand and proper brain function, neurodegenerative disease and/or neuroprotection on the other. The book, compiled by outstanding experts, provides a key reference summarizing the state-of-the-art in creatine and creatine kinase research.
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