Histology Protocols, Hewitson Tim D., Darby Ian A.
Автор: Steven H. Gillespie, Timothy D. McHugh Название: Antibiotic Resistance Protocols ISBN: 160327278X ISBN-13(EAN): 9781603272780 Издательство: Springer Рейтинг: Цена: 91300.00 T Наличие на складе: Есть Описание: This book fully updates and builds upon its first edition. Beginning with chapters on epidemiology and population genetics, it continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance.
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 38730.00 T Наличие на складе: Есть Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 13330.00 T Наличие на складе: Есть Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Gartner, Leslie P., Ph.d. Название: BRS cell biology and histology. 8 ed ISBN: 1496396359 ISBN-13(EAN): 9781496396358 Издательство: Lippincott Williams & Wilkins Рейтинг: Цена: 56630.00 T Наличие на складе: Невозможна поставка. Описание: Be prepared for your courses and board exams with BRS Cell Biology and Histology Acclaimed for its easy-to-read, easy-to-scan outline format, this edition saves time and boosts test-taking confidence. This concise volume distills cell biology and histology facts and concepts commonly covered on course and board exams in a portable, quick-reference format ideal for in-class learning or on-the-go review. Dynamic micrographs, illustrations, full-color tables, and more than 320 board-style multiple-choice review questions with answers reinforce understanding and ensure readiness for exams and the challenges of today's fast-paced clinical practice.
Updated content reflects the latest clinical perspectives and practices related to cell biology and histology
The proven Board Review Series quick-scan outline format helps students make the most of their study time
Updated high-yield Clinical Considerations boxes demonstrate the practical applications of cell biology and histology concepts
Updated board-style review questions with answers ensure confidence on exams
Full-color tables and high-quality illustrations--including 20 new photomicrographs--clarify concepts and clinical details
Accompanying interactive question bank of questions from the chapter and comprehensive exams ready students for the testing experience
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Автор: Turksen Название: Human Embryonic Stem Cell Protocols ISBN: 1607613689 ISBN-13(EAN): 9781607613688 Издательство: Springer Рейтинг: Цена: 97820.00 T Наличие на складе: Есть Описание: Controversy surrounds the study of human embryonic stem cells. In this book, respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells.
Автор: Ian M. Clark Название: Matrix Metalloproteinase Protocols ISBN: 1603272984 ISBN-13(EAN): 9781603272988 Издательство: Springer Рейтинг: Цена: 97820.00 T Наличие на складе: Есть Описание: Detailing proven laboratory techniques for the study of MMPs, this book covers the methods including those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a variety of circumstances.
Автор: Jimenez Gerardo Название: Erk Signaling: Methods and Protocols ISBN: 1493981951 ISBN-13(EAN): 9781493981953 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. How Genetics has Helped Piece Together the MAPK Signaling Pathway Dariel Ashton-Beaucage and Marc Therrien 2. In Vitro Enzyme Kinetics Analysis of EGFR Zhihong Wang and Christine Candelora
3. High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells Nobuo Yoshimoto and Shun'ichi Kuroda 4. Structural Studies of ERK2 Protein Complexes Johannes F. Weijman, Stefan J. Riedl, and Peter D. Mace 5. Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk Tal Goshen-Lago, Dganit Melamed, Arie Admon, and David Engelberg 6. Assaying Activation and Subcellular Localization of ERK in Cells and Tissues Carme Caelles, Carles Bayod, and Melisa Morcillo 7. Detection and Functional Analysis of SUMO-Modified MEK Yuji Kubota and Mutsuhiro Takekawa8. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag Liu Yang and Alexey Veraksa 9. High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates Rona Grossmanand Ze'ev Paroush 10. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE Hidetaka Kosako and Kou Motani 11. Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation Lorena Agudo-Ibaсez, Piero Crespo, and Berta Casar 12. Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions Raffaele Strippoli, Asier Echarri, Miguel Angel del Pozo 13. The Nuclear Translocation of ERK Denise A. Berti and Rony Seger 14. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA) Zijian Tang and Chengkai Dai 15. Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors Yannick Blum, Rafael D. Fritz, Hyunryul Ryu, and Olivier Pertz 16. Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers Hiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, and Masahiro Sokabe 17. Co-Culture Activation of MAP Kinase in Drosophila S2 Cells Josefa Steinhauer 18. Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling Marc K. Saba-El-Leil, Christophe Frйmin, and Sylvain Meloche 19. 3D Organotypic Culture Model to Study Components of ERK Signaling Athina-Myrto Chioni, Rabia Tayba Bajwa, and Richard Grose 20. Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway Carmen G. Lechuga, Lucнa Simуn-Carrasco, Harrys K.C. Jacob, and Matthias Drosten 21. Genome-Wide Analysis of RAS/ERK Signaling Targets Joshua P. Plotnik and Peter C. Hollenhorst 22. Probing Chromatin Modifications in Response to ERK Signaling Ozgur Oksuz and Wee-Wei Tee 23. Analyzing pERK Activation during Planarian Regeneration Susanna Fraguas, Yoshihiko Umesono, Kiyokazu Agata, and FrancescCebriа 24. Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development Jessica Jie Chen and Swathi Arur 25. Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images Bomyi Lim, Carmeline J. Dsilva, Ioannis G. Kevrekidis, and Stanislav Y. Shvartsman
Автор: Narine Sarvazyan Название: Tissue Engineering: Principles, Protocols, and Practical Exercises ISBN: 3030396975 ISBN-13(EAN): 9783030396978 Издательство: Springer Рейтинг: Цена: 69870.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Tissue engineering and regenerative medicine is a new, interdisciplinary branch of science that combines knowledge from numerous scientific fields including biology, biochemistry, physics, chemistry, applied engineering, and medicine. It aims to restore damaged parts of the human body by rebuilding them in vitro using individual building blocks of biological tissues such as cells and the extracellular matrix that surrounds them. The authors hope to spark students’ interest in this exciting new field of science as well as give them a basic knowledge of its terminology. This book is based on a hands-on practical course in tissue engineering conducted by the Fulbright US Scholar recipient, Dr. Narine Sarvazyan (George Washington University, Washington USA). It provides an overview of the core topics of the tissue engineering field, including stem cell differentiation, the role of extracellular matrix and attachment proteins, scaffolds, and culturing of engineered tissues. Each chapter is accompanied by hands-on demonstrations and self-check questions. The text is easily readable for students of all backgrounds and the described protocols can be conducted using common lab equipment. This textbook is also useful for developing undergraduate and graduate courses that teach basic methods and approaches in this promising and rapidly developing field.
Автор: Igor V. Sharakhov Название: Protocols for Cytogenetic Mapping of Arthropod Genomes ISBN: 1138374873 ISBN-13(EAN): 9781138374874 Издательство: Taylor&Francis Рейтинг: Цена: 65320.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Arthropods are important to worldwide agriculture, food safety, human health, and energy production. Besides their practical significance, various species represent excellent model systems for biological investigations of evolution, development, physiology, reproduction, and social interaction. For these reasons, arthropod genomics is receiving increasing attention from researchers around the globe. Protocols for Cytogenetic Mapping of Arthropod Genomes is a collection of up-to-date, detailed protocols for physical chromosome mapping and their applications for studying genome organization and evolution in insects and ticks. This book brings together the expertise of cytogeneticists working on diverse groups of arthropods. Each chapter demonstrates approaches to tissue dissection, chromosome preparation, fluorescence in situ hybridization, and imaging. The book is a timely and complementary effort to the i5K initiative, which will obtain whole-genome sequences for 5,000 insect and related arthropod species. This comprehensive resource provides cytogeneticists with the necessary background and protocols to understand and develop chromosome-based genome assemblies from such whole-genome sequence data.
Автор: Feng Xin-Hua, Xu Pinglong, Lin Xia Название: Tgf-β Signaling: Methods and Protocols ISBN: 1493949934 ISBN-13(EAN): 9781493949939 Издательство: Springer Рейтинг: Цена: 107130.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Chapters provide basic introduction of TGF-ss signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-ss signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway;
Автор: Willars Gary B., Challiss R. a. John Название: Receptor Signal Transduction Protocols: Third Edition ISBN: 1493956256 ISBN-13(EAN): 9781493956258 Издательство: Springer Рейтинг: Цена: 135090.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: As our understanding of G protein-coupled receptor (GPCR) signal transduction continues to grow, we cannot help but be struck by the emerging complexity and the ability of this receptor superfamily to continually surprise us as new facets are discovered. In this third edition of Receptor Signal Transduction Protocols, the expert contributors have taken into account the constant evolution of the GPCR field and dealt with methods that allow researchers to address many of the important rising issues. Serving as a companion to the previous editions, this volume contains thorough revisions of some core chapters, the complete rewriting of others to encompass new technological developments since the publication of the prior editions, and the commissioning of brand new, cutting-edge chapters to expand on previous coverage. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Receptor Signal Transduction Protocols, Third Edition serves the scientific community with a broad collection of the current, practical methodologies in this highly promising field.
Автор: George A Parker Название: Atlas of Histology of the Juvenile Rat ISBN: 0128026820 ISBN-13(EAN): 9780128026823 Издательство: Elsevier Science Рейтинг: Цена: 176290.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Atlas of Histology of the Juvenile Rat should be of interest to toxicologic pathologists, toxicologists, and other biological scientists who are interested in the histomorphology of juvenile rats. For several decades the laboratory rat has been used extensively in nonclinical toxicology studies designed to detect potential human toxicity of drugs, agrochemicals, industrial chemicals, and environmental hazards. These studies traditionally have involved young adult rats that are 8-10 weeks of age as studies are started. It is becoming increasingly apparent that children and young animals may have different responses to drug/chemical exposures, therefore, regulatory agencies are emphasizing toxicology studies in juvenile animals.
While the histologic features of organs from young adult and aged laboratory rats are well known, less is known about the histologic features of organs from juvenile rats. Final histologic maturity of many organs is achieved postnatally, thus immature histologic features must be distinguished from chemical- or drug-related effects. While this postnatal organ development is known to exist as a general concept, detailed information regarding postnatal histologic development is not readily available. The Atlas includes organs that are typically sampled in nonclinical toxicology studies and presents the histologic features at weekly intervals, starting at birth and extending through postnatal day 42.
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