Allostery: Methods and Protocols, Di Paola Luisa, Giuliani Alessandro
Автор: Aron W. Fenton Название: Allostery ISBN: 149395864X ISBN-13(EAN): 9781493958641 Издательство: Springer Рейтинг: Цена: 135090.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Allostery is a vital element in the formation of proteins, binding macromolecules appropriately to their function. This book provides the necessary techniques to study the phenomenon, as well as detailed practical advice to ensure optimal laboratory results.
Автор: Doller Dario, Rotella David Название: Allosterism in Drug Discovery ISBN: 1782624597 ISBN-13(EAN): 9781782624592 Издательство: Royal Society of Chemistry Рейтинг: Цена: 252030.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This book, written by the leaders in this young research area, describes the latest developments in allosterism for drug discovery.
Автор: Jian Zhang; Ruth Nussinov Название: Protein Allostery in Drug Discovery ISBN: 9811387184 ISBN-13(EAN): 9789811387180 Издательство: Springer Рейтинг: Цена: 149060.00 T Наличие на складе: Поставка под заказ. Описание: The book focuses on protein allostery in drug discovery. Allosteric regulation, ?the second secret of life?, fine-tunes virtually most biological processes and controls physiological activities. Allostery can both cause human diseases and contribute to development of new therapeutics. Allosteric drugs exhibit unparalleled advantages compared to conventional orthosteric drugs, rendering the development of allosteric modulators as an appealing strategy to improve selectivity and pharmacodynamic properties in drug leads. The Series delineates the immense significance of protein allostery—as demonstrated by recent advances in the repertoires of the concept, its mechanistic mechanisms, and networks, characteristics of allosteric proteins, modulators, and sites, development of computational and experimental methods to predict allosteric sites, small-molecule allosteric modulators of protein kinases and G-protein coupled receptors, engineering allostery, and the underlying role of allostery in precise medicine. Comprehensive understanding of protein allostery is expected to guide the rational design of allosteric drugs for the treatment of human diseases. The book would be useful for scientists and students in the field of protein science and Pharmacology etc.
Автор: Jian Zhang; Ruth Nussinov Название: Protein Allostery in Drug Discovery ISBN: 9811387214 ISBN-13(EAN): 9789811387210 Издательство: Springer Рейтинг: Цена: 149060.00 T Наличие на складе: Поставка под заказ. Описание: The book focuses on protein allostery in drug discovery. Allosteric regulation, ?the second secret of life?, fine-tunes virtually most biological processes and controls physiological activities. Allostery can both cause human diseases and contribute to development of new therapeutics. Allosteric drugs exhibit unparalleled advantages compared to conventional orthosteric drugs, rendering the development of allosteric modulators as an appealing strategy to improve selectivity and pharmacodynamic properties in drug leads. The Series delineates the immense significance of protein allostery—as demonstrated by recent advances in the repertoires of the concept, its mechanistic mechanisms, and networks, characteristics of allosteric proteins, modulators, and sites, development of computational and experimental methods to predict allosteric sites, small-molecule allosteric modulators of protein kinases and G-protein coupled receptors, engineering allostery, and the underlying role of allostery in precise medicine. Comprehensive understanding of protein allostery is expected to guide the rational design of allosteric drugs for the treatment of human diseases. The book would be useful for scientists and students in the field of protein science and Pharmacology etc.
Автор: Hobman Tom C., Duchaine Thomas F. Название: Argonaute Proteins: Methods and Protocols ISBN: 1493958275 ISBN-13(EAN): 9781493958276 Издательство: Springer Рейтинг: Цена: 135090.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.
Автор: Kragelund Birthe B., Skriver Karen Название: Intrinsically Disordered Proteins: Methods and Protocols ISBN: 1071605232 ISBN-13(EAN): 9781071605233 Издательство: Springer Рейтинг: Цена: 260870.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Intrinsically Disordered Proteins: Methods and Protocols aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules. Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com. Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Автор: Spampinato Santi M. Название: Opioid Receptors: Methods and Protocols ISBN: 1071608835 ISBN-13(EAN): 9781071608838 Издательство: Springer Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Chapters detail transcriptional and post-transcriptional analysis, cellular detection of opioid receptors, analysis of signaling events modulated by opioid receptors, model systems to studying opioid receptor-mediated functions, and behavioral effects mediated by opioid receptors.
Автор: Vila-Perellу Miquel Название: Expressed Protein Ligation: Methods and Protocols ISBN: 1071604333 ISBN-13(EAN): 9781071604335 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Поставка под заказ. Описание: This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
Автор: Kihara Daisuke Название: Protein Function Prediction: Methods and Protocols ISBN: 1493983687 ISBN-13(EAN): 9781493983681 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Preface... Table of Contents... Contributing Authors... 1. Using PFP and ESG Protein Function Prediction Web Servers Qing Wei, Joshua McGraw, Ishita Khan, and Daisuke Kihara 2. GHOSTX: A Fast Sequence Homology Search Tool for Functional Annotation of Metagenomic Data Shuji Suzuki, Takashi Ishida, Masahito Ohue, Masanori Kakuta, and Yutaka Akiyama 3. From Gene Annotation to Function Prediction for Metagenomics Fatemeh Sharifi and Yuzhen Ye 4. An Agile Functional Analysis of Metagenomic Data using SUPER-FOCUS Genivaldo Gueiros Z. Silva, Fabyano A. C. Lopes, and Robert A. Edwards 5. MPFit: Computational Tool for Predicting Moonlighting Proteins Ishita Khan, Joshua McGraw, and Daisuke Kihara 6. Predicting Secretory Proteins with SignalP Henrik Nielsen 7. The ProFunc Function Prediction Server Roman A. Laskowski 8. G-LoSA for Prediction of Protein-Ligand Binding Sites and Structures Hui Sun Lee andWonpil Im 9. Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning Michal Brylinski 10. WATsite2.0 with PyMOL Plugin: Hydration Site Prediction and Visualization Ying Yang, Bingjie Hu, and Markus A. Lill 11. Enzyme Annotation and Metabolic Reconstruction Using KEGG Minoru Kanehisa 12. Ortholog Identification and Comparative Analysis of Microbial Genomes using MBGD and RECOG Ikuo Uchiyama 13. Exploring Protein Function Using the Saccharomyces Genome Database Edith D. Wong 14. Network-Based Gene Function Prediction in Mouse and Other Model Vertebrates using MouseNet Server Eiru Kim and Insuk Lee 15. The FANTOM5 Computation Ecosystem: Genomic Information Hub for Promoters and Active Enhancers Imad Abugessaisa, Shuhei Noguchi, Piero Carninci, and Takeya Kasukawa 16. Multi-Algorithm Particle Simulations with Spatiocyte Satya N. V. Arjunan and Koichi Takahashi
Автор: Greening David W., Simpson Richard J. Название: Serum/Plasma Proteomics: Methods and Protocols ISBN: 1493983806 ISBN-13(EAN): 9781493983803 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Part I Updated Blood Processing and Handling Strategies
1. Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation
Viviana Greco, Cristian Piras, Luisa Pieroni, and Andrea Urbani
2. A Protocol for the Preparation of Cryoprecipitate and Cryo-Depleted Plasma
Rosemary L Sparrow, David W Greening, and Richard J Simpson
3. A Proteomic Protocol for the Preparation of Platelet Concentrates
David W Greening, Richard J Simpson, and Rosemary L Sparrow
Part II Updated Fractionation Strategies for In-depth Blood Proteome Analysis
4. Bead-based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture
Elin Birgersson, Jochen M. Schwenk, and Burcu Ayoglu
5. Affinity Proteomics for Fast, Sensitive, Quantitative Analysis of Proteins in Plasma
John P O'Grady, Kevin W Meyer, and Derrick N Poe
6. Characterization of the Low-molecular Weight Human Plasma Peptidome
David W. Greening and Richard J. Simpson
7. In-depth, Reproducible Analysis of Human Plasma using IgY 14 and SuperMix Immunodepletion
Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, and David W. Speicher
8. Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometry
Dong Huey Cheon, Eun Gyeong Yang, Cheolju Lee, and Ji Eun Lee
9. Identification of Post-Translational Modifications from Serum/Plasma by Immunoaffinity Enrichment and LC-MS/MS Analysis without Depletion of Abundant Proteins
Hongbo Gu, Jianmin Ren, Xiaoying Jia, and Matthew P. Stokes
10. Identification of Core-fucosylated Glycoproteome in Human Plasma
Qichen Cao, Qing Zhao, Xiaohong Qian, and Wantao Ying
Part III Updated Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids
11. Proteomic Analysis of Blood Extracellular Vesicles in Cardiovascular Disease by LC-MS/MS Analysis
Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M. Ruilope, and Maria G. Barderas
12. Targeted Approach For Proteomic Analysis of a Hidden Membrane Protein
Tania Martins-Marques, Sandra I. Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, and Henrique Girao
13. Red Blood Cells in Clinical Proteomics
Ana Sofia Carvalho, Manuel S Rodriguez, and Rune Matthiesen
14. High-throughput Quantitative Lipidomics Analysis of Non-esterified Fatty Acids in Plasma by LC-MS
Nicolas Christinat, Delphine Morin-Rivron, and Mojgan Masoodi
15. Simultaneous Enrichment of Plasma Extracellular Vesicles and Glycoproteome for Studying Disease Biomarkers
Sunil S. Adav and Siu Kwan Sze
16. Lipidomics of Human Blood Plasma by High Resolution Shotgun Mass Spectrometry
Susanne Sales, Oskar Knittelfelder, and Andrej Shevchenko
17. Proteomics Analysis of Circulating Serum Exosomes
Antonius Koller, Purvi Patel, Jenny Kim Kim, and Emily I. Chen
Part IV Antibody-based Discovery and Targeted Proteomics
18. High-density Serum/Plasma Reverse Phase Protein Arrays
Cecilia Hellstrцm, Tea Dodig-Crnkovic, Mun-Gwan Hong, Jochen M. Schwenk, Peter Nilsson, and Ronald Sjцberg
19. Antibody Colocalization Microarray for Cross-reactivity free Multiplexed Protein Analysis
Vйronique Laforte, Pik-Shan Lo, Huiyan Li, and David Juncker
20. Surface Profiling of Extracellular Vesicles From Plasma or Ascites Fluid using Dotscan Antibody Microarrays
Larissa Belov, Susannah Hallal, Kieran Matic, Jerry Zhou, Sandra Wissmueller, Nuzhat Ahmed, Sumaiya Tanjil, Stephen P. Mulligan, O. Giles Best, Richard J. Simpson, and Richard I. Christopherson
Автор: Fahie Monifa A. V. Название: Nanopore Technology: Methods and Protocols ISBN: 1071608053 ISBN-13(EAN): 9781071608050 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nanopore Technology: Methods and Protocols, with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are working toward further understanding this key field of research.
Автор: McGuckin Michael A., Thornton David J. Название: Mucins: Methods and Protocols ISBN: 1493958860 ISBN-13(EAN): 9781493958863 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.
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