Cd95: Methods and Protocols, Legembre Patrick

Автор: Patrick Legembre
Название: CD95
ISBN: 1493967789 ISBN-13(EAN): 9781493967780
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

Production of the Non-apoptotic Metalloprotease-cleaved CD95L and its Cytotoxic Recombinant Counterpart Designed Ig-CD95L.- CD95 Stimulation with CD95L and DISC Analysis.- Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8.- In vitro Evaluation of the Apoptosis Function in Human Activated T cells.- Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition.- Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization.- Generation and Application of Bioluminescent CD95 Ligand Fusion Proteins.- CD95-mediated Calcium Signaling.- CD95-mediated Proton Regulation.- Study of the CD95-mediated Non-apoptotic Signaling Pathway - PI3K.- Organelle Separation and Cell Signaling.- Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L.- Isolation of Lipid Rafts through Discontinuous Sucrose Gradient Centrifugation and Fas/CD95 Death Receptor Localization in Raft Fractions.- Quantifying CD95/cl-CD95L Implications in Cell Mechanics And Membrane Tension by Atomic Force Microscopy Based Force Measurements.- Sketching of CD95 Oligomers by In Silico Investigations.- Site-specific Detection of Tyrosine Phosphorylated CD95 Following Protein Separation by Conventional and Phospho-Protein Affinity SDS-PAGE.- Detection of S-acylated CD95 by Acyl-biotin Exchange.- Exploration of Fas S-nitrosylation by the Biotin Switch Assay.- Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L.- Liquid Chromatography-High Resolution Mass Spectrometry Method to Study Sphingolipid Metabolism Changes in Response to CD95L.- CD95 and the MRL-lpr Mouse Model.

Автор: Dissmeyer Nico, Schnittger Arp
Название: Plant Kinases: Methods and Protocols
ISBN: 149395721X ISBN-13(EAN): 9781493957217
Издательство: Springer
Рейтинг:
Цена: 111790.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.

Автор: Lanzuolo Chiara, Bodega Beatrice
Название: Polycomb Group Proteins: Methods and Protocols
ISBN: 1493981811 ISBN-13(EAN): 9781493981816
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

Mapping the Function of Polycomb Proteins.- Chromatin Immunoprecipitation.- Chromatin preparation and Chromatin Immuno-Precipitation from Drosophila embryos.- ChIP-seq data processing for PcG proteins and associated histone modifications.- Analysis of single locus replication timing in asynchronous cycling cells.- Non coding RNA interplay with the genome.- RIP: RNA Immunoprecipitation.- Capture Hybridization Analysis of DNA Targets.- Identification of RNA-protein interactions through in-vitro RNA pull-down assays.- Understanding RNA-chromatin interactions using Chromatin Isolation by RNA Purification (ChIRP).- Analysis RNA-seq and non coding RNA.- The dynamics of Polycomb complexes.- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics.- Analysis of endogenous protein interactions of Polycomb-group of proteins in mouse embryonic stem cells.- Determination of Polycomb group of proteins compartmentalization through chromatin fractionation procedure.- An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteins in High-Throughput Microscopy Images.- Polymer physics of the large-scale structure of chromatin.- Chromosome Conformation Capture in Drosophila.- Chromosome Conformation Capture in primary human cells.- Determination of high-resolution 3D chromatin organization using Circular Chromosome Conformation Capture (4C-seq).- Chromosome Conformation Capture on Chip (4C): Data Processing.- In vivo models to address the function of Polycomb Group Proteins.- A rapid TALEN assembly protocol.- Following the motion of Polycomb bodies in living Drosophila embryos.- Reprogramming of Somatic Cells towards Pluripotency by Cell Fusion.- Imaginal discs transplantation in Drosophila.- Isolation and culture of muscle stem cells.

Автор: Comai Lucio, Katz Jonathan E., Mallick Parag
Название: Proteomics: Methods and Protocols
ISBN: 1493982907 ISBN-13(EAN): 9781493982905
Издательство: Springer
Рейтинг:
Цена: 139750.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome.

Автор: Rйdini Franзoise
Название: Proteoglycans: Methods and Protocols
ISBN: 1493958798 ISBN-13(EAN): 9781493958795
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Now recognized as a reservoir for growth factors and cytokines modulating cell activation status and turnover, proteoglycans have stimulated great amount of interest and research. In Proteoglycans: Methods and Protocols, experts in the field examine issues of basic concepts and up-to-date analysis methods for proteoglycan and glycosaminoglycan (GAG) at the protein and saccharide levels. The volume continues with the multifunctional aspect of proteoglycans, highlighted through three relevant examples of proteoglycans with highly different strucutures: serglycin, aggrecan, and heparin sulphate proteoglycans. The final chapter describes proteoglycan involvement in the pathogenesis of various disorders and their potential therapeutic value in osteo-articular diseases. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Proteoglycans: Methods and Protocols is an ideal guide for scientists attempting to pursue further research in this vital and complex area of study.

Автор: Krдmer Oliver H.
Название: Hdac/Hat Function Assessment and Inhibitor Development: Methods and Protocols
ISBN: 1493982265 ISBN-13(EAN): 9781493982264
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro.

Автор: Pulido Rafael
Название: Protein Tyrosine Phosphatases: Methods and Protocols
ISBN: 1493981277 ISBN-13(EAN): 9781493981274
Издательство: Springer
Рейтинг:
Цена: 102480.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.

Автор: Ray H. Gavin
Название: Cytoskeleton Methods and Protocols
ISBN: 1617796492 ISBN-13(EAN): 9781617796494
Издательство: Springer
Рейтинг:
Цена: 135090.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: In this cutting-edge book, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research. There is emphasis on animal, plant, protist, and fungal model systems.

Автор: Pompa Andrea, De Marchis Francesca
Название: Unconventional Protein Secretion: Methods and Protocols
ISBN: 1493981447 ISBN-13(EAN): 9781493981441
Издательство: Springer
Рейтинг:
Цена: 46570.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

Part I: Background

Автор: Takigawa Masaharu
Название: Ccn Proteins: Methods and Protocols
ISBN: 1493981978 ISBN-13(EAN): 9781493981977
Издательство: Springer
Рейтинг:
Цена: 116450.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques.

Автор: Kister Alexander E.
Название: Protein Supersecondary Structures: Methods and Protocols
ISBN: 1493991604 ISBN-13(EAN): 9781493991600
Издательство: Springer
Рейтинг:
Цена: 139750.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

Автор: Abbott D. Wade, Lammerts Van Bueren Alicia
Название: Protein-Carbohydrate Interactions: Methods and Protocols
ISBN: 1493983326 ISBN-13(EAN): 9781493983322
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques.