Clark Ian M. Matrix Metalloproteinase Protocols 9781493956227

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Автор: Clark Ian M.
Название: Matrix Metalloproteinase Protocols
ISBN: 9781493956227
Издательство: Springer
Классификация:

Междисциплинарные науки

Биохимия

Энзимология

Учебные материалы: естественные науки: общие вопросы

ISBN-10: 1493956221
Обложка/Формат: Paperback
Страницы: 492
Вес: 0.87 кг.
Дата издания: 23.08.2016
Серия: Methods in molecular biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 15 tables, black and white; xii, 492 p.
Размер: 25.40 x 17.78 x 2.59 cm
Читательская аудитория: Professional & vocational
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.
Дополнительное описание: MMPs and TIMPs: An Overview.- Metalloproteases and the Degradome.- Mouse Models of MMP and TIMP Function.- Expression and Purification of MMPs and TIMPs.- Expression of Recombinant MMP-28 in Mammalian Cells.- Expression of Recombinant Matrix Metalloprotei

Matrix Metalloproteinase Protocols, Clark Ian M.