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Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods, McGenity Terry J., Timmis Kenneth N., Nogales Balbina


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Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina
Название:  Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods
ISBN: 9783662569825
Издательство: Springer
Классификация:

ISBN-10: 3662569825
Обложка/Формат: Paperback
Страницы: 204
Вес: 0.38 кг.
Дата издания: 22.04.2018
Серия: Springer protocols handbooks
Язык: English
Издание: Softcover reprint of
Иллюстрации: 20 illustrations, color; 20 illustrations, black and white; x, 204 p. 40 illus., 20 illus. in color.
Размер: 25.40 x 17.78 x 1.17 cm
Читательская аудитория: Professional & vocational
Подзаголовок: Single-cell and single-molecule methods
Ссылка на Издательство: Link
Рейтинг:
Поставляется из: Германии
Описание: Introduction: Single cell biotechnology for uncultured microorganisms.- Single-cell cultivation in microfluidic devices.- Single-cell bacterial bioreporter assays to measure hydrocarbons.- Whole genome amplification of single bacterial cells.- Detection of specific mRNA molecules in hydrocarbon-degrading bacteria.- Localisation of proteins within intact bacterial cells using fluorescent protein fusions.- Raman-FISH.- Single-cell metabolomics.- Marrying single molecules to single cells: Protocols for the study of the bacterial proto-ring components essential for division.- Single cell biotechnology for uncultured microorganisms.- Single cell and systems biology tools for biofuel production.- Detection of protein-synthesizing microorganisms in the environment via bioorthogonal non-canonical amino acid tagging (BONCAT).- Flow cytometric determination of microbial abundances and its use to obtain indices of community structure and relative activity.- Protocols for visualizing horizontal gene transfer in Gram-negative bacteria through natural competence.


Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods

Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina
Название: Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods
ISBN: 3662569841 ISBN-13(EAN): 9783662569849
Издательство: Springer
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Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds.

Hydrocarbon and Lipid Microbiology Protocols

Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga
Название: Hydrocarbon and Lipid Microbiology Protocols
ISBN: 366252791X ISBN-13(EAN): 9783662527917
Издательство: Springer
Рейтинг:
Цена: 139750.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function.

Hydrocarbon and Lipid Microbiology Protocols: Petroleum, Hydrocarbon and Lipid Analysis

Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina
Название: Hydrocarbon and Lipid Microbiology Protocols: Petroleum, Hydrocarbon and Lipid Analysis
ISBN: 3662570831 ISBN-13(EAN): 9783662570838
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function.

Hydrocarbon and Lipid Microbiology Protocols

Автор: McGenity
Название: Hydrocarbon and Lipid Microbiology Protocols
ISBN: 3662504332 ISBN-13(EAN): 9783662504338
Издательство: Springer
Рейтинг:
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

This Volume presents a comprehensive series of generic protocols for the genetic and genomic analysis of prokaryotic isolates. Genetic methods for functional analyses employ the latest cloning vectors, gene fusion methods and transposon mutagenesis systems, as well as systems for introducing protease-cleavage sequences into permissive sites in proteins under investigation. Genomic methods described include protocols for transcriptomics, shotgun proteomics, interactomics, metabolic profiling, and lipidomics. Bioinformatic tools for genome annotation, transcriptome display and the integration of transcriptomic data into genome-scale metabolic reconstructions are described. Protocols for 13C-based metabolic flux determinations and analysis of the hierarchical and metabolic regulation of fluxes through pathways are included. The Volume thus enables investigators to functionally analyse an isolate over the entire cellular range spanning the gene, the genome, the transcript repertoire, the proteome, the interactome, the metabolic network with its nodes and their regulatory hierarchies, and the metabolic fluxes and their physiological controls.Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols: Ultrastructure and Imaging

Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina
Название: Hydrocarbon and Lipid Microbiology Protocols: Ultrastructure and Imaging
ISBN: 3662569833 ISBN-13(EAN): 9783662569832
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells.

Hydrocarbon and Lipid Microbiology Protocols: Synthetic and Systems Biology - Applications

Автор: McGenity Terry J., Timmis Kenneth N., Nogales Fernбndez Balbina
Название: Hydrocarbon and Lipid Microbiology Protocols: Synthetic and Systems Biology - Applications
ISBN: 3662569817 ISBN-13(EAN): 9783662569818
Издательство: Springer
Рейтинг:
Цена: 106010.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume presents protocols for systems and synthetic biology applications in the field of hydrocarbon and lipid microbiology.

Hydrocarbon and Lipid Microbiology Protocols: Field Studies

Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina
Название: Hydrocarbon and Lipid Microbiology Protocols: Field Studies
ISBN: 3662571153 ISBN-13(EAN): 9783662571156
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
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Описание: Introduction.- Marine Systems.- Coastline/Intertidal Systems.- Terrestrial Systems.- Petroleum Reservoirs.- Aquifers/Groundwater.- Freshwater Lakes, Rivers.- Investigating the Biodegradation of Volatile Hydrocarbons in Diverse Environments.

Hydrocarbon and Lipid Microbiology Protocols: Statistics, Data Analysis, Bioinformatics and Modelling

Автор: McGenity Terry J., Timmis Kenneth N., Nogales Fernбndez Balbina
Название: Hydrocarbon and Lipid Microbiology Protocols: Statistics, Data Analysis, Bioinformatics and Modelling
ISBN: 3662570009 ISBN-13(EAN): 9783662570005
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Introduction to computer-assisted analysis in lipid and hydrocarbon microbiology.- Application of Ecological Network Theory.- Statistical tools for data analysis.- Statistical tools for study design and replication.- MG-RAST, a metagenomics service for analysis of microbial community structure and function.- Using QIIME to evaluate the microbial communities within hydrocarbon environments.- Biodegradation Prediction Tools.- Predicting protein interactions.- Syntax and Semantics of Coding in Python.- Protocols for calculating reaction kinetics and thermodynamics.- Modelling the environmental fate of hydrocarbons during bioremediation.

Hydrocarbon and Lipid Microbiology Protocols

Автор: McGenity
Название: Hydrocarbon and Lipid Microbiology Protocols
ISBN: 3662504278 ISBN-13(EAN): 9783662504277
Издательство: Springer
Рейтинг:
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

This Volume addresses the pros and cons of oligonucleotide probes, primers and primer combinations, and importantly considers how to design the best tools for the microbial taxa and/or processes being investigated. Individual chapters focus on the design of primers targeting genes that code for enzymes associated with the following functions: degradation of aromatic, aliphatic and chlorinated hydrocarbons under aerobic and anaerobic conditions, methanogenesis, methane oxidation, and the nitrogen cycle.Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga
Название: Hydrocarbon and Lipid Microbiology Protocols
ISBN: 3662531070 ISBN-13(EAN): 9783662531075
Издательство: Springer
Рейтинг:
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: < Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function.

Hydrocarbon and Lipid Microbiology Protocols

Автор: Terry J. McGenity; Kenneth N. Timmis; Balbina Noga
Название: Hydrocarbon and Lipid Microbiology Protocols
ISBN: 3662527766 ISBN-13(EAN): 9783662527764
Издательство: Springer
Рейтинг:
Цена: 121110.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers.Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols: Genetic, Genomic and System Analyses of Communities

Автор: McGenity Terry J., Timmis Kenneth N., Nogales Balbina
Название: Hydrocarbon and Lipid Microbiology Protocols: Genetic, Genomic and System Analyses of Communities
ISBN: 3662570629 ISBN-13(EAN): 9783662570623
Издательство: Springer
Рейтинг:
Цена: 93160.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities.


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