Yeast Protocols, Xiao Wei

Автор: Jeffrey S. Smith; Daniel J. Burke
Название: Yeast Genetics
ISBN: 1493951823 ISBN-13(EAN): 9781493951826
Издательство: Springer
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Цена: 102480.00 T
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Описание: Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse.

Автор: Bin Liu
Название: Yeast Surface Display
ISBN: 1493927477 ISBN-13(EAN): 9781493927470
Издательство: Springer
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Цена: 130430.00 T
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Описание: Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications.

Автор: Stephen G. Oliver; Juan I. Castrillo
Название: Yeast Systems Biology
ISBN: 1493997386 ISBN-13(EAN): 9781493997381
Издательство: Springer
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Цена: 144410.00 T
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Описание: This second edition volume expands on the previous edition with a look at the latest advances in techniques to study yeast and its core set of interactions, modules, architectures, and network dynamics that are common in all eukaryotes. The chapters in this book are organized into Four Parts: Part One provides readers with an update on the development of novel experimental and computational approaches to yeast systems biology; Part Two explores high-throughput methods used to study yeast epigenome, transcriptome, proteome, and metabolome; Part Three talks about computational systems biology, and focuses on data management, dynamic modeling, constraint-based models of metabolic networks, and multi-level ‘omics data; while Part Four looks at experimental platforms that utilize yeast to model systemic human diseases such as Alzheimer’s and Parkinson’s diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Yeast System Biology: Methods and Protocols, Second Edition is a valuable tool for graduate students, post-doctoral researchers, and experts who are interested in learning about the latest developments in the study of yeast.

Автор: Becskei Attila
Название: Yeast Genetic Networks: Methods and Protocols
ISBN: 1493957775 ISBN-13(EAN): 9781493957774
Издательство: Springer
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Цена: 135090.00 T
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Описание: Regulation of gene expression, a major determinant of gene activity, occupies a central place in molecular biology. Yeast Genetic Networks: Methods and Protocols covers approaches to the systems biological analysis of small-scale gene networks in yeast. Divided into four convenient sections, this detailed volume discusses the methods used to analyze gene expression quantitatively, presents a collection of mathematical and computational tools to analyze stochasticity, adaptation, sensitivity in signal transmission, and oscillations in gene expression, provides instructional methods on how to utilize the tools of quantitative genetics to identify genes that regulate stochasticity and oscillations in gene expression, and concludes with a section devoted to the analysis of conserved gene expression systems and networks in different fungal species. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Yeast Genetic Networks: Methods and Protocols serves as an ideal and valuable resource for both novices and experienced researchers.

Автор: Wei Xiao
Название: Yeast Protocols
ISBN: 1493942778 ISBN-13(EAN): 9781493942770
Издательство: Springer
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Цена: 102480.00 T
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Описание:

1. Basic Investigations in Saccharomyces cerevisiae

Brendan Curran and Virginia Bugeja

2. Isolation of Yeast Nucleic Acids

Michael Biss, Michelle Hanna and Wei Xiao

3. Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry

Ting Wei and Heping Dai

4. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

R. Daniel Gietz

5. Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe

Johannes H. Hegemann, Sven Boris Heick, Jennifer Pцhlmann, Markus Langen and Ursula Fleig

6. See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry

Changhui Deng and Andrew N. Krutchinsky

7. Yeast Two Hybrid Liquid Screening

Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz

8. Targeted Mutagenesis of a Specific Gene in Yeast

Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz

9. Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae

Kai Stefan Dimmer

10. Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy

Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie

11. Assays for Autophagy II: Mitochondrial Autophagy

Tomotake Kanki and Koji Okamoto

12. Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction

Danielle Hagstrom and Changle Ma

13. Yeast Survival and Growth Assays

Xin Xu, Amanda Lambrecht, and Wei Xiao

14. Spontaneous Mutagenesis Assay

Susan Blackwell, Michelle Hanna, and Wei Xiao

15. Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene

Bernard A. Kunz

16. piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe

Jun Li and Li-Lin Du

17. Replicative and Chronological Life Span Assays

Spike D. L. Postnikoff and Troy A. A. Harkness

18. Detection of Protein Arginine Methylation in Saccharomyces cerevisiae

Christopher Aa. Jackson and Michael C. Yu

19. Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae

Lindsay G. Ball and Wei Xiao

20. Ligand Engineering Using Yeast Surface Display

Benjamin J. Hackel

21. Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay

Kris Barreto and C. Ronald Geyer

Автор: Wei Xiao
Название: Yeast Protocols
ISBN: 1493907980 ISBN-13(EAN): 9781493907984
Издательство: Springer
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Цена: 139750.00 T
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Автор: J.F.T. Spencer; D.M. Spencer; A.R.W. Smith
Название: Yeast Genetics
ISBN: 1461254930 ISBN-13(EAN): 9781461254935
Издательство: Springer
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Цена: 111790.00 T
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Описание: With contributions by numerous experts

Автор: Watson Dean
Название: Microbiology: Yeast and Fungi
ISBN: 1682861481 ISBN-13(EAN): 9781682861486
Издательство: Неизвестно
Цена: 155550.00 T
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Описание: This book contains some path-breaking studies in the field of microbiology with particular emphasis on yeast and fungi. It traces the progress of this field and highlights some of its key concepts and applications. The chapters included in this book focus on structures and classification of yeast and fungi, their physiology, applications of yeast and fungi in different fields that of utmost significance and bound to provide incredible insights to readers. Some of the diverse topics covered in this book address the varied branches that fall under this category. The book aims to provide a comprehensive overview of the discipline as well as the recent developments and researches in the field of yeast and fungal science.

Автор: Alistair J.P. Brown; Mick F. Tuite; John E.G. McCa
Название: Protein Synthesis and Targeting in Yeast
ISBN: 3642849237 ISBN-13(EAN): 9783642849237
Издательство: Springer
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Цена: 113190.00 T
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Описание: Proceedings of the NATO Advanced Research Workshop on Protein Synthesis and Targeting in Yeast, held at Canterbury, Kent, UK, July 4-8, 1992

Автор: Juan I. Castrillo; Stephen G. Oliver
Название: Yeast Systems Biology
ISBN: 1493961357 ISBN-13(EAN): 9781493961351
Издательство: Springer
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Цена: 153720.00 T
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Описание: This book presents an up-to-date view of yeast as a model eukaryote for systems biology studies. It provides practical, easy to use methodologies and includes key tips and vital implementation advice from the experts.

Автор: Fr?d?ric Devaux
Название: Yeast Functional Genomics
ISBN: 1493930788 ISBN-13(EAN): 9781493930784
Издательство: Springer
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Цена: 139750.00 T
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Описание: This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes.

Автор: Tulasi Satyanarayana; Gotthard Kunze
Название: Yeast Diversity in Human Welfare
ISBN: 9811026203 ISBN-13(EAN): 9789811026201
Издательство: Springer
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Цена: 186330.00 T
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Описание: This book brings together and updates the latest information on the diversity of yeasts, their molecular features and their applications in the welfare of mankind.