DNA Electrophoresis: Methods and Protocols, Hanada Katsuhiro
Автор: Didenko Vladimir V. Название: In Situ Detection of DNA Damage / Methods and Protocols ISBN: 0896039528 ISBN-13(EAN): 9780896039520 Издательство: Springer Рейтинг: Цена: 38730.00 T Наличие на складе: Есть Описание: In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Автор: Zachariou Michael Название: Affinity Chromatography / Methods and Protocols ISBN: 1588296598 ISBN-13(EAN): 9781588296597 Издательство: Springer Рейтинг: Цена: 13330.00 T Наличие на складе: Есть Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.
Автор: Kwok Pui-Yan Название: Single Nucleotide Polymorphisms / Methods and Protocols ISBN: 0896039684 ISBN-13(EAN): 9780896039681 Издательство: Springer Цена: 38730.00 T Наличие на складе: Нет в наличии. Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Автор: Didenko Vladimir V. Название: Fast Detection of DNA Damage: Methods and Protocols ISBN: 149398411X ISBN-13(EAN): 9781493984114 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.
Автор: Aphasizhev Ruslan Название: RNA and DNA Editing: Methods and Protocols ISBN: 1493958216 ISBN-13(EAN): 9781493958214 Издательство: Springer Рейтинг: Цена: 111790.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.
Автор: Ke Yonggang, Wang Pengfei Название: 3D DNA Nanostructure: Methods and Protocols ISBN: 1493982052 ISBN-13(EAN): 9781493982059 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Part I: DNA/RNA Structure Design
1. Designed 3D DNA Crystals
Nadrian C. Seeman, Ruojie Sha, Jens Birktoft, Jianping Zheng, Wenyan Liu, Tong Wang, and Chengde Mao
2. Three-Dimensional DNA Nanostructures Assembled from DNA Star Motifs
Cheng Tian and Chuan Zhang
3. Design of Wireframe DNA Nanostructures-DNA Gridiron
Dongran Han
4. Complex DNA Brick Assembly
Luvena Ong, Yonggang Ke, and Peng Yin
5. Computer-Aided Design of RNA Origami Structures
Steffen L. Sparvath, Cody W. Geary, and Ebbe S. Andersen
6. Assembling RNA Nanoparticles
Shou-Jun Xiao
Part II: Applications of DNA Structures
7. DNA Functionalization of Nanoparticles
Fang Lu and Oleg Gang
8. Purification Techniques for Three-Dimensional DNA Nanostructures
Travis A. Meyer
9. DNA Nanostructure as Smart Carriers for Drug Delivery
Xiangyuan Ouyang, Jie Chao, Shao Su, and Chunhai Fan
10. DNA G-Quadruplex-Based Assay of Enzyme Activity
Zhuoliang Liu, Kaiyu He, Wang Li, Xin Liu, Xiahong Xu, Zhou Nie, and Shouzhuo Yao
11. Assembly of Multi-Enzyme Cascade on a DNA Origami Nanostructure
Jinglin Fu and Tianran Li
12. Lipid Membrane Encapsulation of a 3D DNA NanoOctahedron
Steven D. Perrault and William M. Shih
13. DNA-PAINT Super-Resolution Imaging for Nucleic Acid Nanostructures
Mingjie Dai
14. Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins
John Min, William M. Shih, and Gaлtan Bellot
15. Direct Nanofabrication Using DNA Nanostructure
Feng Zhou and Haitao Liu
16. Confined Growth of Metal Nanoparticles Within 3D DNA Origami Molds
Wei Sun and Jie Shen
17. DNA-Directed Self-Assembly of Highly Ordered and Dense Single Walled Carbon Nanotube Arrays
Hareem Maune and Si-ping Han
18. A Proximity-Based Programmable DNA Nanoscale Assembly Line
Xiaoyan Zhang, Xiaoqiang Ding, Jianzhou Zou, and Hongzhou Gu
19. DNA Walkers as Transport Vehicles of Nanoparticles Along a Carbon Nanotube Track
Jing Pan, Tae-Gon Cha
Автор: Almeida de Sousa Название: DNA Vaccines: Methods and Protocols ISBN: 1071608711 ISBN-13(EAN): 9781071608715 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Автор: Bill Roslyn M. Название: Recombinant Protein Production in Yeast: Methods and Protocols ISBN: 1493958712 ISBN-13(EAN): 9781493958719 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Автор: Blair Camila Название: Molecular Cytogenetics: Methods and Protocols ISBN: 1632417995 ISBN-13(EAN): 9781632417992 Издательство: Неизвестно Рейтинг: Цена: 155570.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Bridging the Information Gap. Imagining the healthcare of the future is an interesting exercise, and although nobody can predict precisely what systems might operate in ten years time, the possibilities which already exist can give us a clue as to how healthcare may be managed by 2030.This book presents papers from the conference Healthcare of the Future, held in Biel/Bienne, Switzerland, on 5 April 2019. The conference reflects some of the results of a two year multi-stakeholder Swiss research program in medical informatics. The research program, which began in 2016, saw 25 stakeholders cooperating for an integrated cross-sectoral
Автор: Kozlov Sergei V. Название: ATM Kinase: Methods and Protocols ISBN: 1493983504 ISBN-13(EAN): 9781493983506 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
1. Assaying Radiosensitivity of Ataxia-Telangiectasia
Hailiang Hu, Shareef Nahas, and Richard A. Gatti
2. Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Fluorography-Based Methodology
Amanda W. Kijas and Martin F. Lavin
3. ATM Gene Mutation Detection Techniques and Functional Analysis
Guillaume Rieunier, Catherine Dubois D'Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, and Marc-Henri Stern
4. An HTRF(R) Assay for the Protein Kinase ATM
Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, and Andrew Plater
6. Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci
Yi Chieh Lim
7. Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy
Keiji Suzuki
8. Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging
Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla
9. Zn(II)-Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein
Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
10. Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry
Mark E. Graham, Martin F. Lavin, and Sergei V. Kozlov
11. Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS)
Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, and Ryo Otomo
12. Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies
Sun Yingli and Du Fengxia
13. Identification of ATM-Interacting Proteins by Co-Immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays
Amanda L. Bain, Janelle L. Harris, and Kum Kum Khanna
14. ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning-Cytometry
Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, and Zbigniew Darzynkiewicz
15. Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling
Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, and Amanda G. Paulovich
16. Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosph
Автор: Mathй Ewy, Davis Sean Название: Statistical Genomics: Methods and Protocols ISBN: 1493980831 ISBN-13(EAN): 9781493980833 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This volume expands on statistical analysis of genomic data by discussing cross-cutting groundwork material, public data repositories, common applications, and representative tools for operating on genomic data. Statistical Genomics: Methods and Protocols is divided into four sections. The first section discusses overview material and resources that can be applied across topics mentioned throughout the book. The second section covers prominent public repositories for genomic data. The third section presents several different biological applications of statistical genomics, and the fourth section highlights software tools that can be used to facilitate ad-hoc analysis and data integration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step-by-step, readily reproducible analysis protocols, and tips on troubleshooting and avoiding known pitfalls. Through and practical, Statistical Genomics: Methods and Protocols, explores a range of both applications and tools and is ideal for anyone interested in the statistical analysis of genomic data.
Автор: William S. M. Wold; Ann E. Tollefson Название: Adenovirus Methods and Protocols ISBN: 1617376698 ISBN-13(EAN): 9781617376696 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This updated book is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. New chapters address innovative, exciting areas of emphasis in Ad research.
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