Ra the Mighty: The Great Tomb Robbery, Greenfield A. B.
Автор: Greenfield Lauren Название: Generation Wealth: Lauren Greenfield ISBN: 0714872121 ISBN-13(EAN): 9780714872124 Издательство: Phaidon Press Ltd. Рейтинг: Цена: 77080.00 T Наличие на складе: Есть Описание: A highly anticipated monograph from the internationally acclaimed documentary photographer and filmmaker
Автор: Greenfield Edward A. Название: Antibodies a Laboratory Manual, Second Edition ISBN: 1936113813 ISBN-13(EAN): 9781936113811 Издательство: Неизвестно Рейтинг: Цена: 160930.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: It has been 25 years since the now-classic Antibodies by Ed Harlow and David Lane appeared. While some things remain the same, many have changed. This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. The remaining chapters contain all new protocols and topics and reflect the progress in how antibodies are studied and used since the appearance of the original edition. These include: � antibody purification and storage � engineering antibodies, including use of degenerate oligonucleotides, 5'-RACE, phage display, and mutagenesis � extensive labeling techniques � new immunoblotting protocols � the latest screening and labeling techniques As Dr. Greenfield notes in his preface to this second edition: "The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. Everything we needed to know to make a monoclonal antibody was all there, neatly packaged in an easy-to-understand book...the second edition is intended to provide the necessary information and protocols to assist investigators with their first monoclonal antibody effort as well as to provide guidance for more experiences antibody makers who are having some difficulties with a particular project." I have the first edition. Why should I buy the second edition? While the core of the first edition appears in updated and recast form in the second edition, more than half of the second edition contains entirely new information and protocols to reflect the changes in the field since the first edition. To whom is this edition addressed? Is this a book for immunologists? As with the first edition, the second edition provides information and protocols for both the neophyte and the experienced investigator. It dissects the mysteries of producing the antibodies and reagents you need for your experiments with the needs of molecular biologists in mind. It is the perfect book for new members of your lab as it has sufficient background information to provide context as well as clear, step-by-step instructions for performing the needed experiments-both the why and the how for these techniques. And if things go wrong, there is extensive troubleshooting to diagnose the problem. Are the protocols in this book better than those available for download on the Web? Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have.
Автор: Greenfield A. B. Название: Ra the Mighty: Cat Detective ISBN: 0823440273 ISBN-13(EAN): 9780823440276 Издательство: Random House (USA) Цена: 15630.00 T Наличие на складе: Невозможна поставка. Описание: An egotistical royal cat and his sidekick track a jewelry thief in this funny new series that reads like Tom and Jerry crossed with The Egypt Game. Witty writing and zany illustrations, for kids who love to laugh. Ra relishes his role as the Pharaoh's beloved--and spoiled--cat. So when an amulet goes missing from the palace, Ra has no interest in solving the mystery. He would rather enjoy his snacks and nap in the warm Egyptian sun. But Ra's friend Khepri, a wise and industrious scarab beetle, insists on investigating in order to save the young servant girl who has been framed for the crime. Once Ra gets going, he decides that being a Great Detective isn't so bad; he certainly doesn't mind being hailed as "Protector of the Weak and Defender of Justice." But can a lazy cat and a dung-obsessed beetle really crack a mystery? The comically mismatched duo is on the case "An ingenious mystery full of entertaining details for fans of ancient Egypt."--Paula Harrison, author of the Rescue Princesses series
Автор: Greenfield Ellen Название: Come from Nowhere ISBN: 0979352762 ISBN-13(EAN): 9780979352768 Издательство: Неизвестно Цена: 14930.00 T Наличие на складе: Есть у поставщика Поставка под заказ.
Автор: Greenfield, Robert Название: Cedar cage ISBN: 1910903051 ISBN-13(EAN): 9781910903056 Издательство: Неизвестно Рейтинг: Цена: 20830.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
No.2 National Bestseller (run-up Christmas 2017) - Jarrold
The boathouse had been built by an Edwardian aristocrat - from a single cedar of Lebanon - as a retreat, maybe even a cage, for his glamorous American bride. But in 2008, just weeks after moving into this dream home, Bertie starts to feel uneasy about living inside someone else's fantasy.
Obsession takes hold when he becomes convinced that a carpenter commissioned to fulfil Lord James Newton-Grey's vision, was murdered in the boathouse. His investigations immerse him in a sinister web of family secrets, as tangled and treacherous as the Norfolk marshlands that lie beyond his windows.
Bertie's own dark past plays tag with the present, driving him to the edge of madness, when he is forced to confront a chilling truth about himself - delivered by an unexpected visitor on Christmas Day.
Readers of The Cedar Cage - like its characters - must make up their own minds: is Bertie's strange, unquenchable imagination running riot... or has something genuinely supernatural been at work?
Author of the bestseller, Samphire Coast
'Robert Greenfield's cleverly crafted thriller is a real page-turner ' - Christopher Warwick, royal biographer and broadcaster.
In the sixteenth century, one of the world's most precious commodities was cochineal, a legendary red dye treasured by the ancient Mexicans and sold in the great Aztec marketplaces, where it attracted the attention of the Spanish conquistadors. Shipped to Europe, the dye created a sensation, producing the brightest, strongest red the world had ever seen. Soon Spain's cochineal monopoly was worth a fortune. As the English, French, Dutch, and other Europeans joined the chase for cochineal -- a chase that lasted for more than three centuries -- a tale of pirates, explorers, alchemists, scientists, and spies unfolds. A Perfect Red evokes with style and verve this history of a grand obsession, of intrigue, empire, and adventure in pursuit of the most desirable color on earth.
--J. H. Elliott, University of Oxford
Автор: Susan Greenfield Название: The Private Life of the Brain ISBN: 0141007206 ISBN-13(EAN): 9780141007205 Издательство: Random House - Penguin Рейтинг: Цена: 9670.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: As our minds develop we create a personalized inner world based on our experiences. But during periods of intense emotion, such as anger, fear or euphoria, we can literally lose our mind, returning to the mental state we experienced as infants. This book intends to answer how our unique sense of self is created.
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