Bernd Sokolowski Auditory and Vestibular Research 9781493980956

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Автор: Bernd Sokolowski
Название: Auditory and Vestibular Research
ISBN: 9781493980956
Издательство: Springer
Классификация:

Анатомия

Медицинская генетика

Молекулярная биология

ISBN-10: 1493980955
Обложка/Формат: Paperback
Страницы: 547
Вес: 0.97 кг.
Дата издания: 2016
Серия: Methods in Molecular Biology
Язык: English
Издание: Softcover reprint of
Иллюстрации: 90 tables, color; 13 tables, black and white; 89 illustrations, color; 31 illustrations, black and white; xvi, 547 p. 120 illus., 89 illus. in color.
Размер: 254 x 178 x 29
Читательская аудитория: General (us: trade)
Основная тема: Biomedicine
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание:

Helios(R) Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates.- Tol2-Mediated Delivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation.- A Rapid, Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation Enables Efficient Gene Transfer to the Developing Mouse Inner Ear.- Generation of Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannoma and Meningioma.- RNA Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning and Expression Profiling with RNA-Seq and Microarrays .- Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory System.- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research.- Protein Quantification of the Developing Cochlea by Mass Spectrometry.- Ultrastructural Identification and Colocalization of Interacting Proteins in the Murine Cochlea by Post-Embedding Immunogold Transmission Electron Microscopy.- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins.- The Single-Molecule Approach to Membrane Protein Stoichiometry.- Visualization of Live Cochlear Stereocilia at a Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy.- Design and Construction of a Cost-Effective Spinning Disk System for Live Imaging of Inner Ear Tissue.- Neuroanatomical Tracing Techniques: History, State of the Art, and Future Developments.- Detection of Excitation and Inhibition in the Auditory System Using Fluorescence Immunohistochemistry and High Resolution Fluorescence Microscopy.- Rapid and Semi-Automated Extraction of Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks.-Organotypic Culture of the Mouse Cochlea from Embryonic Day 12 to the Neonate.- Quantifying Spiral Ganglion Neurite and Schwann Behavior on Micropatterned Polymer Substrates.- The Use of Human Whartons Jelly Cells for Cochlear Tissue Engineering.- Nanotechnology in Auditory Research: Membrane Electromechanics in Hearing.- Overview of Nanoparticle Based Delivery for Treatment of Inner Ear Disorders.- Development of Cell-based High-Throughput Chemical Screens for Protection Against Cisplatin-induced Ototoxicity.-Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques.- Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo.- Method for Dissecting the Auditory Epithelium (Basilar Papilla) in Developing Chick Embryos.- Whole Cell Patch Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti.- Glass Probe Stimulation of Hair Cell Stereocilia.- A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells.- In Vitro Functional Assessment of Adult Spiral Ganglion Neurons (SGNs).- Mapping Auditory Circuits with Photostimulation of Caged Glutamate.

Auditory and Vestibular Research, Bernd Sokolowski