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Handbook of Analysis of Oligonucleotides and Related Products, 


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Название:  Handbook of Analysis of Oligonucleotides and Related Products
Перевод названия: Справочник по анализу олигонуклеотидов и родственных продуктов
ISBN: 9781138198456
Издательство: Taylor&Francis
Классификация:




ISBN-10: 1138198455
Обложка/Формат: Paperback
Страницы: 511
Вес: 0.38 кг.
Дата издания: 19.10.2016
Язык: English
Иллюстрации: 76 tables, black and white; 266 illustrations, black and white
Размер: 176 x 246 x 38
Читательская аудитория: Tertiary education (us: college)
Ключевые слова: Molecular biology, MEDICAL / Biotechnology,MEDICAL / Pharmacy,SCIENCE / Chemistry / Analytic
Основная тема: Molecular Biology
Ссылка на Издательство: Link
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Поставляется из: Европейский союз
Описание: Oligonucleotides represent one of the most significant pharmaceutical breakthroughs in recent years, showing great promise as diagnostic and therapeutic agents for malignant tumors, cardiovascular disease, diabetes, viral infections, and many other degenerative disorders. The Handbook of Analysis of Oligonucleotides and Related Products is an essential reference manual on the practical application of modern and emerging analytical techniques for the analysis of this unique class of compounds. A strong collaboration among thirty leading analytical scientists from around the world, the book provides readers with a comprehensive overview of the most commonly used analytical techniques and their advantages and limitations in assuring the identity, purity, quality, and strength of an oligonucleotide intended for therapeutic use. Topics discussed include: Strategies for enzymatic or chemical degradation of chemically modified oligonucleotides toward mass spectrometric sequencing Purity analysis by chromatographic or electrophoretic methods, including RP-HPLC, AX-HPLC, HILIC, SEC, and CGE Characterization of sequence-related impurities in oligonucleotides by mass spectrometry and chromatography Structure elucidation by spectroscopic methods (IR, NMR, MS) as well as base composition and thermal melt analysis (Tm) Approaches for the accurate determination of molar extinction coefficient of oligonucleotides Accurate determination of assay values Assessment of the overall quality of oligonucleotides, including microbial analysis and determination of residual solvents and heavy metals Strategies for determining the chemical stability of oligonucleotides The use of hybridization techniques for supporting pharmacokinetics and drug metabolism studies in preclinical and clinical development Guidance for the presentation of relevant analytical information towards meeting current regulatory expectations for oligonucleotide therapeutics This resource provides a practical guide for applying state-of-the-art analytical techniques in research, development, and manufacturing settings.

Protocols for Oligonucleotides and Analogs

Автор: Sudhir Agrawal
Название: Protocols for Oligonucleotides and Analogs
ISBN: 0896032817 ISBN-13(EAN): 9780896032811
Издательство: Springer
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Цена: 163040.00 T
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Описание: When first conceived, not only was the aim of Protocols for Oligo- nucleotides and Analogs to provide wide coverage of the ohgonuc- otide chemistry field for readers who are well versed within the field, but also to give investigators just entering into the field a new perspective.

Oligonucleotide-Based Therapies

Автор: Olof Gissberg; Rula Zain; Karin E. Lundin
Название: Oligonucleotide-Based Therapies
ISBN: 149399669X ISBN-13(EAN): 9781493996698
Издательство: Springer
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Цена: 158380.00 T
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Описание: This book provides a collection of comprehensive, up-to-date, and broadly applicable guides to the research and development fields of oligonucleotide (ON) therapeutics. Covering topics from the study of antisense and anti-gene effects to oligonucleotides in the context of drug discovery and development, the volume explores a wide-ranging and useful spectrum of methods and protocols needed to take full advantage of therapeutic applications involving ONs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Oligonucleotide-Based Therapies: Methods and Protocols aims to be a great aid in the laboratory as well as an ideal reference guide when designing antisense and anti-gene oligonucleotides for therapeutic applications.


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