Nicholas Catsimpoolas Biological and Biomedical Applications of Isoelectric Focusing 9781461341833

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Автор: Nicholas Catsimpoolas
Название: Biological and Biomedical Applications of Isoelectric Focusing
ISBN: 9781461341833
Издательство: Springer
Классификация:

Биохимия

ISBN-10: 1461341833
Обложка/Формат: Paperback
Страницы: 352
Вес: 0.52 кг.
Дата издания: 31.12.2011
Серия: Biological Separations
Язык: English
Размер: 234 x 156 x 20
Основная тема: Life Sciences
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: It has now been over a decade since isoelectric focusing became estab- lished as a valuable addition to our arsenal of physical methods for separa- tion of proteins and other amphoteric substances. The high resolving power of the technique and its unique ability to separate as well as concentrate have been amply demonstrated in several international symposia. The past few years have led to the wide acceptance of standardized methodologies for analytical and preparative purposes. These procedures afford rapid, reproducible separations that have led to a dramatic increase in our under- standing of many areas of biological and biomedical research. Conse- quently, we considered it desirable to review some of the important appli- cations of the isoelectric focusing technique. To accomplish this, we have solicited the participation of noted authorities to highlight major progress in their fields of expertise. Thus, the material in this book will emphasize recent advances in knowledge rather than methodological aspects, except when special procedures are reviewed. Nicholas Catsimpoolas James W. Drysdale vii Contents Chapter J Isoelectric Focusing of Human Saliva, Cerebrospinal Fluid, and Urine Josie A. Beeley I. Introduction I II. Isoelectric Focusing of Saliva . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 A. Gel Rods and Thin Layer Gels . . . . . . . . . . . . . . . . . . . . . . . . 2 B. Liquid Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 C. a-Amylases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 D. Immunoglobulins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 E. Blood Group Specific Substances . . . . . . . . . . . . . . . . . . . . . 11 F. Vitamin B12 Binding Proteins (Cobalophilins) . . . . . . . . . . . 12 G. Other Glycoproteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 H. Lysozyme . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 I. Specific Salivary Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 J. Dental Plaque . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 K. Other Proteins . . . . . . . . . . . . . . ., . . . . . . . . . . . . . . . . . . . . . . 16 III. Isoelectric Focusing of Cerebrospinal Fluid . . . . . . . . . . . . . . . .

Biological and Biomedical Applications of Isoelectric Focusing, Nicholas Catsimpoolas