RT-PCR Protocols, Nicola King

Автор: Zachariou Michael
Название: Affinity Chromatography / Methods and Protocols
ISBN: 1588296598 ISBN-13(EAN): 9781588296597
Издательство: Springer
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Цена: 13330.00 T
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Описание: Thirty-eight years after its introduction, affinity chromatography remains a key tool in th armory of separation techniques available to separation and interaction scientists.  Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

Автор: Kwok Pui-Yan
Название: Single Nucleotide Polymorphisms / Methods and Protocols
ISBN: 0896039684 ISBN-13(EAN): 9780896039681
Издательство: Springer
Цена: 38730.00 T
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Описание: Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

Автор: Dmitry M. Kolpashchikov (Editor), Yulia V. Gerasim
Название: Nucleic Acid Detection: Methods and Protocols (Methods in Molecular Biology)
ISBN: 1627035346 ISBN-13(EAN): 9781627035347
Издательство: Springer
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Цена: 111790.00 T
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Описание: This Methods in Molecular Biology book details approaches for the detection of DNA and RNA including recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, step-by-step, reproducible protocols, materials lists, tips and more.

Автор: LeDoux, Joseph (Ed.)
Название: Gene therapy protocols: Vol 2 design and characterization of gene transfer vectors
ISBN: 160327247X ISBN-13(EAN): 9781603272476
Издательство: Springer
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Цена: 158380.00 T
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Описание: The field of gene therapy has undergone remarkable advances, promising to impact human healthcare in the 21st century. This book provides methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression.

Автор: Mark van der Giezen
Название: Protein Targeting Protocols
ISBN: 1617377279 ISBN-13(EAN): 9781617377273
Издательство: Springer
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Цена: 144410.00 T
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Описание: In this updated volume, experts from around the world provide the latest protocols for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Emphasis is placed on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems.

Автор: William S. M. Wold; Ann E. Tollefson
Название: Adenovirus Methods and Protocols
ISBN: 1617376698 ISBN-13(EAN): 9781617376696
Издательство: Springer
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Цена: 130430.00 T
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Описание: This updated book is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. New chapters address innovative, exciting areas of emphasis in Ad research.

Автор: Jeff Braman
Название: In Vitro Mutagenesis Protocols
ISBN: 1493956663 ISBN-13(EAN): 9781493956661
Издательство: Springer
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Цена: 130430.00 T
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Описание: In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.

Автор: Nicola King
Название: RT-PCR Protocols
ISBN: 1493956825 ISBN-13(EAN): 9781493956821
Издательство: Springer
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Цена: 139750.00 T
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Описание: Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.

Автор: Roberto Biassoni; Alessandro Raso
Название: Quantitative Real-Time PCR
ISBN: 149394665X ISBN-13(EAN): 9781493946655
Издательство: Springer
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Цена: 93160.00 T
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Описание: Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ? Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation. --

Автор: LeDoux, Joseph (Ed.)
Название: Gene therapy protocols: Volume 1 preliminary entry 2071 production and in vivo applications of gene transfer vectors
ISBN: 1588299031 ISBN-13(EAN): 9781588299031
Издательство: Springer
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Цена: 135090.00 T
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Описание: Describes methods for the production of viral and non-viral gene transfer vectors, as well as protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering.

Автор: Shulin Li; Jeffry Cutrera; Richard Heller; Justin
Название: Electroporation Protocols
ISBN: 149395492X ISBN-13(EAN): 9781493954926
Издательство: Springer
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Цена: 111790.00 T
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Описание: Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA.

Автор: Allan V. Kalueff; Adam Michael Stewart
Название: Zebrafish Protocols for Neurobehavioral Research
ISBN: 1493960024 ISBN-13(EAN): 9781493960026
Издательство: Springer
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Цена: 135090.00 T
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Описание: Zebrafish play an integral role in biomedical research, enabling scientists to examine physiological mechanisms and pathways relevant to human pathogenesis and its therapy. Written by experts, this volume sets out protocols for both larval and adult models.