Signal Transduction Immunohistochemistry, Alexander E. Kalyuzhny
Автор: Buchwalow, Igor B. Bocker, Werner Название: Immunohistochemistry ISBN: 3642046088 ISBN-13(EAN): 9783642046087 Издательство: Springer Рейтинг: Цена: 143480.00 T Наличие на складе: Есть Описание: This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Автор: Krauss Название: Biochemistry of Signal Transduction and Regulation5e ISBN: 3527333665 ISBN-13(EAN): 9783527333660 Издательство: Wiley Рейтинг: Цена: 106650.00 T Наличие на складе: Невозможна поставка. Описание: Originally based on a graduate course taught by the author, this true classic has once again been extensively updated to incorporate key new findings in biological signaling. With over half of the content re-written, plus 70 brand new and 50 revised figures, this is the most up-to-date textbook on signaling available anywhere.
Автор: Kalyuzhny Название: Signal Transduction Immunohistochemistry ISBN: 1617790230 ISBN-13(EAN): 9781617790232 Издательство: Springer Рейтинг: Цена: 135090.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.
Название: Technical aspects of toxicological immunohistochemistry ISBN: 1493915150 ISBN-13(EAN): 9781493915156 Издательство: Springer Рейтинг: Цена: 139750.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This authoritative volume examines immunohistochemical methods aimed at investigating the toxicologic pathology of rodent, non-human primate and aquatic animal tissues.
Автор: Muin S.A. Tuffaha; Hans Guski; Glen Kristiansen Название: Immunohistochemistry in Tumor Diagnostics ISBN: 3319535765 ISBN-13(EAN): 9783319535760 Издательство: Springer Рейтинг: Цена: 110100.00 T Наличие на складе: Невозможна поставка. Описание: This book offers a comprehensive yet concise overview of immunoprofile of tumors and antibodies used in contemporary surgical pathology, and provides diagnostic algorithms for approaching tumor diagnostics. Immunohistochemistry has become the most important ancillary technique in diagnostic pathology in the last 20 years, and unlike most books on tumor diagnostics, this volume discusses in details immunohistochemical biomarkers, diagnostic approaches and their pitfalls, as well as the immunoprofile of common tumors throughout all systems of human body. With numerous color figures and detailed flowcharts, it appeals to all pathologists be they young residents in training who want a brief introduction to this technique, or specialists in need of a reliable and comprehensive reference resource in tumors diagnostics.
Автор: Wang Rui Название: Signal Transduction and the Gasotransmitters / NO, CO, and H2S in Biology and Medicine ISBN: 1588293491 ISBN-13(EAN): 9781588293497 Издательство: Springer Рейтинг: Цена: 158380.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Distinguished researchers and clinicians review the biological and biomedical aspects of gasotransmitters, emphasizing signaling transduction mechanisms in general, and ion channel regulation in particular. The authors discuss the endogeneous metabolism and regulation of gasotransmitters, their toxicological profiles and biological actions, and their interactions in terms of their production and effects. The physiological roles of NO, CO, and H2S in the regulation of the cardiovascular, neuronal, and gastrointestinal systems, as well as of cell metabolism, are also reviewed, along with the interaction of the gastrotransmitters with KATP,KCa voltage-gated Ca2+, voltage-gated Na+, and cyclic nucleotide-gated ion channels.
Автор: Alexander E. Kalyuzhny Название: Signal Transduction Immunohistochemistry ISBN: 1493967576 ISBN-13(EAN): 9781493967575 Издательство: Springer Рейтинг: Цена: 130430.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание:
Preface... Table of Contents... Contributing Authors...
1. Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications Kathy Brumbaugh, Wen-Chie Liao, J. P. Houchins, Jeff Cooper, and Steve Stoesz
2. Antibodies That Work Again and Again and Again Kameron Simpson and Michael Browning 3. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods Biji T. Kurien and R. Hal Scofield 4. Acquisition and Post-Processing of Immunohistochemical Images Jerry Sedgewick 5. Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction Jessica Kalra and Jennifer Baker 6. EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis Christianne Bandeira-Melo, Ligia Almeida Paiva, Natбlia R. T. Amorim, Peter F. Weller, and Patricia T. Bozza 7. Preservation of Specific Protein Signaling States using Heat Based Stabilizor System Mats Borйn 8. Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX using Primary Antibodies Raised in the Same Host Species Jodi Hagen, David Schwartz, and Alexander E. Kalyuzhny 9. Culture, Transfection and Immunocytochemical Analysis of Primary Macrophages Charles A. Warwick and Yuriy M. Usachev 10. Phenotyping CD4+ hTh2 Cells by Flow Cytometry: Simultaneous Detection of Transcription Factors, Secreted Cytokines and Surface Markers Chris Goetz, Li-Jen Peng, Birte Aggeler, and Jody Bonnevier 11. A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy Aishwarya Sathyanarayan 12. Apoptotic Bodies: Selective Detection in Extracellular Vesicles Paul Hauser, Sha Wang, and Vladimir V. Didenko 13. Activation and Differentiation of Mesenchymal Stem Cells Pravin J. Mishra and Debabrata Banerjee 14. High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues using Secondary Antibody Conjugated to Polymer-HRP Michael Grahek, Ana Ptak, and Alexander E. Kalyuzhny 15. Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry David Schwartz, Michael Grahek, Yingwei He, Wei Wang, Jennifer Nguyen, and Alexander E. Kalyuzhny 16. Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D Candace C. Minchew and Vladimir V. Didenko 17. Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment Jessica Kalra and Jennifer Baker 18. Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors Jacob Ricca, Mesruh Turkekul, Afsar Barlas, Dmitry Yarilin, Sho Fujisawa, Ning Fan, Matthew Brendel, Dmitriy Zamarin, Jedd D. Wolchok, and Katia Manova-Todorova 19. Automated Double In Situ Detection of Mouse LGR5 mRNA and Lysozyme Protein in Examining the Neighboring Cell Types of the Mouse Intestinal Crypt Mesruh Turkekul, Afsar Barlas, Dmitry Yarilin, Sho Fujisawa, Ning Fan, Matthew Brendel, and Katia Manova-Todorova 20. Automated High Content Screening of g--H2AX Expression in HeLa Cells Nate Hopp, Jodi Hagen, Birte Aggeler, and Alexander E. Kalyuzhny
Автор: Kalyuzhny, Alexander E. Название: Immunohistochemistry Essential Elements and Beyond ISBN: 3319308912 ISBN-13(EAN): 9783319308913 Издательство: Springer Рейтинг: Цена: 102480.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: Short Historic Overview.- Primary Antibodies.- Choosing Commercial Suppliers of Primary Antibodies.- Detection and Visualization of Antigens.- Ancillary Reagents for IHC.- Preparing Tissues for IHC Staining.- Staining Protocols.- Microscopy Examination and Collection of Digital Images and Their Presentation.- Concluding Remarks.
Автор: Igor B. Buchwalow; Werner B?cker Название: Immunohistochemistry: Basics and Methods ISBN: 364242502X ISBN-13(EAN): 9783642425028 Издательство: Springer Рейтинг: Цена: 121110.00 T Наличие на складе: Есть у поставщика Поставка под заказ. Описание: This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Cellular Signal Processing offers a unifying view of cell signaling based on the concept that protein interactions act as sophisticated data processing networks that govern intracellular and extracellular communication. It is intended for use in signal transduction courses for undergraduate and graduate students working in biology, biochemistry, bioinformatics, and pharmacology, as well as medical students. The text is organized by three key topics central to signal transduction: the protein network, its energy supply, and its evolution. It covers all important aspects of cell signaling, ranging from prokaryotic signal transduction to neuronal signaling, and also highlights the clinical aspects of cell signaling in health and disease. This new edition includes expanded coverage of prokaryotes, as well as content on new developments in systems biology, epigenetics, redox signaling, and small, non-coding RNA signaling.
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