MicroRNA Detection and Target Identification, Tamas Dalmay

Автор: Zhang
Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells
ISBN: 1607617684 ISBN-13(EAN): 9781607617686
Издательство: Springer
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Цена: 149060.00 T
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Описание: This cutting-edge text will be a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and exciting areas of research such as regenerative medicine and cancer.

Автор: Zhiguo Wang; Baofeng Yang
Название: MicroRNA Expression Detection Methods
ISBN: 3642425186 ISBN-13(EAN): 9783642425189
Издательство: Springer
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Цена: 191550.00 T
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Описание: This book provides comprehensive descriptions of the innovative strategies and methodologies for detecting miRNA expression as well as their application in miRNA research and their potential as tools for clinical diagnosis and prognosis.

Автор: Fazlul H. Sarkar
Название: MicroRNA Targeted Cancer Therapy
ISBN: 3319051334 ISBN-13(EAN): 9783319051338
Издательство: Springer
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Цена: 156720.00 T
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Описание: The most attractive feature of miRNAs is that one miRNA can regulate many target genes (mRNAs) and thus miRNA targeted therapy is highly promising because multiple genes could be regulated by targeting a single miRNA, which becomes very important for the killing of highly heterogeneous populations of cancer cells within a tumor mass.

Автор: Zhiguo Wang
Название: MicroRNA Interference Technologies
ISBN: 3642101518 ISBN-13(EAN): 9783642101519
Издательство: Springer
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Цена: 156720.00 T
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Описание: This book presents an overview of up-to-date knowledge of MicroRNAs (miRNAs) biology and their potential as therapeutic targets for human disease. It also gives an introduction of the new concept of miRNA interference (miRNAi).

Автор: Malik Yousef; Jens Allmer
Название: miRNomics: MicroRNA Biology and Computational Analysis
ISBN: 1627037470 ISBN-13(EAN): 9781627037471
Издательство: Springer
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Цена: 102480.00 T
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Описание: In miRNomics: MicroRNA Biology and Computational Analysis, expert researchers in the field present an overview of the current state of the art and aim to put the respective areas of research into a larger perspective.

Автор: Baohong Zhang; Edmund J. Stellwag
Название: RNAi and microRNA-Mediated Gene Regulation in Stem Cells
ISBN: 149395749X ISBN-13(EAN): 9781493957491
Издательство: Springer
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Цена: 130430.00 T
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Описание: Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination. In RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications, expert authors from laboratories across the globe contribute an accessible compendium of up-to-date, proven methods focused on the study of the titular topic. Divided into three sections, the book first gives a brief introduction to RNAi and miRNAs in stem cells, with a focus on the current status of research and future perspectives, then it continues with detailed methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNA-based shRNA, and miRNA expression analysis using qRT-PCR. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications serves as a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and the exciting areas of medicine, including regenerative medicine, aging, cancer, and neurological disorders, that can be advanced through this expanding area of research.

Автор: Xueji Zhang; Haifeng Dong; Yaping Tian
Название: MicroRNA Detection and Pathological Functions
ISBN: 3662472929 ISBN-13(EAN): 9783662472927
Издательство: Springer
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Цена: 51230.00 T
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Описание: This book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions. This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA research. Dr. Xueji Zhang and Dr. Haifeng Dong are Professors at the School of Chemistry & Biological Engineering, University of Science & Technology Beijing (USTB), China. Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School, China.

Автор: Fazlul H. Sarkar
Название: MicroRNA Targeted Cancer Therapy
ISBN: 3319376063 ISBN-13(EAN): 9783319376066
Издательство: Springer
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Цена: 130590.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: The most attractive feature of miRNAs is that one miRNA can regulate many target genes (mRNAs) and thus miRNA targeted therapy is highly promising because multiple genes could be regulated by targeting a single miRNA, which becomes very important for the killing of highly heterogeneous populations of cancer cells within a tumor mass.

Автор: Jian-Bing Fan
Название: Next-Generation MicroRNA Expression Profiling Technology
ISBN: 1493962175 ISBN-13(EAN): 9781493962174
Издательство: Springer
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Цена: 135090.00 T
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Описание: The rapid pace of research in this field continues to drive technical advances that make methods more sensitive and applicable. This volume features many new methodologies described by leading researchers, including reproducible laboratory protocols.

Автор: Wei Wu
Название: MicroRNA and Cancer
ISBN: 1493957740 ISBN-13(EAN): 9781493957743
Издательство: Springer
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Цена: 111790.00 T
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Описание: Authoritative and cutting-edge, this book provides the basic principles of experimental and computational methods for microRNA study in cancer research. It provides a firm grounding for those who wish to further develop their own applications.

Автор: Rani
Название: MicroRNA Profiling
ISBN: 1493965220 ISBN-13(EAN): 9781493965229
Издательство: Springer
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Цена: 121110.00 T
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Описание: This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

Автор: Shao-Yao Ying
Название: MicroRNA Protocols
ISBN: 1493976001 ISBN-13(EAN): 9781493976003
Издательство: Springer
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Цена: 111790.00 T
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Описание:

This volume focuses on the analysis of miRNA and its many components such as targets and expression profiling, regulation of gene expression, miRNA detection in biofluids, and its application in cancer and wound healing. The chapters in this book explore topics that's cover biogenesis, isolation and profiling of miRNA, exosomal miRNAs, and the study of the functional significance of miRNAs. The chapters also cover screening miRNAs in mR-302/367 induced iPSCs, exosomal miRNAs from iPSC-derived cardiomyocytes proliferation, and the recently discovered sugar-like RNA glycylglycerins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, MicroRNA Protocols, Third Edition is a valuable resource that provides researchers with a comprehensive study of miRNAs and its numerous components and uses.