Lipidomics of Stem Cells, Alice P?bay; Raymond C.B. Wong

Автор: Xianlin Han
Название: Lipidomics: Comprehensive Mass Spectrometry of Lipids
ISBN: 1118893123 ISBN-13(EAN): 9781118893128
Издательство: Wiley
Рейтинг:
Цена: 131950.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: Covers the area of lipidomics from fundamentals and theory to applications

• Presents a balanced discussion of the fundamentals, theory, experimental methods and applications of lipidomics
• Covers different characterizations of lipids including Glycerophospholipids; Sphingolipids; Glycerolipids and Glycolipids; and Fatty Acids and Modified Fatty Acids
• Includes a section on quantification of Lipids in Lipidomics such as sample preparation; factors affecting accurate quantification; and data processing and interpretation
• Details applications of Lipidomics Tools including for Health and Disease; Plant Lipidomics; and Lipidomics on Cellular Membranes

Автор: Paul Wood
Название: Lipidomics
ISBN: 1493969447 ISBN-13(EAN): 9781493969449
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells such as lymphocytes and oocytes.

Автор: Sanjoy K. Bhattacharya
Название: Lipidomics
ISBN: 1493969951 ISBN-13(EAN): 9781493969951
Издательство: Springer
Рейтинг:
Цена: 130430.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание:

Preface...
Table of Contents...
Contributing Authors...

1. Lipid Sample Preparation for Biomedical Research
Ravin Sajnani and Katyayini Aribindi

2. Lipid Extraction Techniques for Stable Isotope Analysis and Ecological Assays
Kyle H. Elliot, James D. Roth, and Kevin Crook

3. Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells
Vineet K. Gupta and Sulagna Banerjee

4. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation
Blake E. Hopiavuori, Dustin E. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman

5. Sample Preparation and Analysis for Imaging Mass Spectrometry
Genea Edwards, Annia Mesa, Robert I. Vazquez-Padron, Paul Kowalski, and Sanjoy K. Bhattacharya

6. Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLC and Enzymatic Assay Based Method
Christopher E. Pedigo, Sandra M. Merscher, and Alessia Fornoni

7. High-Performance Chromatographic Separation of Cerebrosides
Renaud Sicard and Ralf Landgraf

8. Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid Chromatography
Gabriel B. Gugiu

9. Utility of Moderate and High Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification
Maria del Carmen Piqueras

10. A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue using Liquid-Chromatography High-Resolution Tandem Mass Spectrometry
Candice Z. Ulmer, Rainey Patterson, Jeremy Koelmel, Timothy J. Garrett, and Richard A. Yost

11. Combined Use of MALDI-TOF Mass Spectrometry and ³¹PNMR Spectrometry for Analysis of Phospholipids
Jenny Schrцter, Yulia Popkova, Rosmarie Sь , and Jьrgen Schiller

12. Global Monitoring of Mammalian Lipidome by Quantitative Shotgun Lipodomics
Inger dum Nielsen, Kenji Maeda, and Mesut Bilgin

13. Bioinformatics Pertinent to Lipid Analysis in Biological Samples
Justin Ma, Ulises Arbelo, Yenifer Guerra, Katyayini Aribindi, Sanjoy K. Bhattacharya, and Daniel Pelaez

14. LC-MS-Based Lipidomics and Automated Identification of Lipids using the LipidBlast In-Silico MS/MS Library
Tomas Cajka and Oliver Fiehn

15. Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa
Pallavi Lahiri and Dipankar Ghosh

16. Analysis of Fatty Acid and Cholesterol Content from Detergent-Resistant and Detergent-Free Membrane Microdomains
Mark E. McClellan and Michael H. Elliott

17. Computational Functional Analysis of Lipid Metabolic Enzymes
Carolina Bagnato, Arjen Ten Have, Marнa B. Prados, and Marнa B. Beligni

18. Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells
Evan B. Stubbs, Jr.

19. Purification and Validation of Lipid Transfer Proteins
Matti A. Kjellberg, Anders P.E. Backman, Anna Mцuts, and Peter Mattjus

20. Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells
Rania Leventis and John R. Silvius

21. Sonication-Based Basic Protocol for Liposome Synthesis
Roberto Mendez and Santanu Banerjee

22. On Electrochemical Methods for Determination of Protein-Lipid Interaction
Zhiping Hu and Yanli Mao

23. Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate
Joseph L. Wilkerson and Nawajes A. Mandal

Автор: Datta
Название: Statistical Analysis of Proteomics, Metabolomics, and Lipidomics Data Using Mass Spectrometry
ISBN: 3319458078 ISBN-13(EAN): 9783319458076
Издательство: Springer
Рейтинг:
Цена: 149060.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This book presents an overview of computational and statistical design and analysis of mass spectrometry-based proteomics, metabolomics, and lipidomics data. This contributed volume provides an introduction to the special aspects of statistical design and analysis with mass spectrometry data for the new omic sciences. The text discusses common aspects of design and analysis between and across all (or most) forms of mass spectrometry, while also providing special examples of application with the most common forms of mass spectrometry. Also covered are applications of computational mass spectrometry not only in clinical study but also in the interpretation of omics data in plant biology studies.Omics research fields are expected to revolutionize biomolecular research by the ability to simultaneously profile many compounds within either patient blood, urine, tissue, or other biological samples. Mass spectrometry is one of the key analytical techniques used in these new omic sciences. Liquid chromatography mass spectrometry, time-of-flight data, and Fourier transform mass spectrometry are but a selection of the measurement platforms available to the modern analyst. Thus in practical proteomics or metabolomics, researchers will not only be confronted with new high dimensional data types—as opposed to the familiar data structures in more classical genomics—but also with great variation between distinct types of mass spectral measurements derived from different platforms, which may complicate analyses, comparison, and interpretation of results.

Автор: Christie William, Han Xianlin, Christie W. W.
Название: Lipid Analysis: Isolation, Separation, Identification and Lipidomic Analysis (Revised)
ISBN: 0081014651 ISBN-13(EAN): 9780081014653
Издательство: Elsevier Science
Рейтинг:
Цена: 190890.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf.Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics.The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.