Mootz Split Inteins 9781493964499

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Автор: Mootz
Название: Split Inteins
ISBN: 9781493964499
Издательство: Springer
Классификация:

Биохимия

Белки

ISBN-10: 1493964496
Обложка/Формат: Hardback
Страницы: 270
Вес: 0.73 кг.
Дата издания: 2017
Серия: Methods in Molecular Biology
Язык: English
Издание: 1st ed. 2017
Иллюстрации: 38 tables, color; 36 illustrations, color; 26 illustrations, black and white; xiv, 270 p. 62 illus., 36 illus. in color.
Размер: 254 x 178 x 18
Читательская аудитория: General (us: trade)
Основная тема: Life Sciences
Подзаголовок: Methods and Protocols
Ссылка на Издательство: Link
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Поставляется из: Германии
Описание: This volume focuses on applications of split inteins, and the progress that has been made in the past 5 years on discovery and engineering of fast and more efficient split inteins. The first few chapters in Split Inteins: Methods and Protocols explore new techniques on how to use split inteins for affinity purification of overproduced proteins, and split-intein based technologies to prepare cyclic peptides and proteins. The next few chapters discuss semisynthetic protein trans-splicing using one synthetic intein piece, synthetic intein-extein pieces used to deliver other cargos for chemical modification both of purified proteins and of proteins in living cells, as well as isotopic labeling of proteins for NMR studies, and a discussion on how protein block copolymers can be generated by protein trans-splicing to form protein hydrogels. The last few chapters deal with intein applications in transgenic plants and conditional inteins that can be regulated in artificial ways by small molecules or light, a cassette-based approach to quickly test many intein insertion positions, and a computational approach to predict new intein split sites (the approach also works for other proteins). Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Split Inteins: Methods and Protocols is a valuable resource that will provide guidance toward possibilities of split intein applications, explore proven and detailed protocols adaptable to various research projects, and inspire new method developments.
Дополнительное описание: Affinity Purification of Proteins in Tag-Free Form: Split-Intein-Mediated Ultra-Rapid Purification (SIRP).- Purification of Microbially Expressed Recombinant Proteins via a Dual ELP Split Intein System.- Intracellular Production of Cyclic Libraries with

Split Inteins, Mootz